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  4. Recombinant Danio rerio E3 ubiquitin-protein ligase mib1 (mib1), partial

Recombinant Danio rerio E3 ubiquitin-protein ligase mib1 (mib1), partial

  • 中文名稱：
    斑馬魚mib1重組蛋白
  • 貨號：
    CSB-YP773868DIL
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    斑馬魚mib1重組蛋白
  • 貨號：
    CSB-EP773868DIL
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    斑馬魚mib1重組蛋白
  • 貨號：
    CSB-EP773868DIL-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    斑馬魚mib1重組蛋白
  • 貨號：
    CSB-BP773868DIL
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    斑馬魚mib1重組蛋白
  • 貨號：
    CSB-MP773868DIL
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    mib1; mib; E3 ubiquitin-protein ligase mib1; EC 2.3.2.27; Protein mind bomb; RING-type E3 ubiquitin transferase mib1
  • 種屬：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. It thereby participates in many processes regulated by the Notch signaling pathway, such as midline cell fate specification prior to germ layer formation, patterning of sensory cell differentiation in the ear, neurogenesis of the hindbrain and commitment to a secretory fate in the intestine. Essential for early embryonic development.
  • 基因功能參考文獻(xiàn)：
    1. Data reported the first genome-wide analysis and transcriptome profiling of the new mib(nn2002) in Zebrafish. These results suggested that the defects in the somitogenesis were related to the down-regulated segmentation genes, such as foxc1a, smyhc1, myod1 and mylpfa. PMID: 29126925
    2. Epb41l5 competes with Delta as a substrate for Mib1 to coordinate specification and differentiation of neurons. PMID: 27510968
    3. indicate that miR-10 regulates the angiogenic behaviour in a Notch-dependent manner by directly targeting mib1 PMID: 26825552
    4. forms of DeltaD that have the MID can activate Notch in trans and suppress early neurogenesis when mRNA encoding it is ectopically expressed in zebrafish embryos. PMID: 26020642
    5. Mib and Mib2 interact with Fih-1 that is involved in the hypoxia signaling pathway. PMID: 24714733
    6. Data indicate a general down-regulation of Notch signaling genes necessary for proper brain development in mind bomb-mutant zebrafish embryos. PMID: 21688347
    7. mind bomb mutants have downregulation of several genes necessary for GABA-mediated signaling. Spontaneous seizures are seen in these animals. PMID: 20943912
    8. Mosaic analysis indicates that the bronchiomotor neurons patterning and fusion defects in the mib hindbrain arise non-cell autonomously. PMID: 14579383
    9. The three functionally distinct modules of Mind bomb work cooperatively to regulate Notch signaling by associating with, ubiquitinating, and internalizing Delta. PMID: 15013799
    10. Zebrafish Mib and Mib2 are colocalized in transfected cells and function redundantly in regulating Notch signaling in embryos. PMID: 17331493
    11. The mib ( ta52b ) mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway. PMID: 19005681
    12. Mib encodes a RING ubiquitin ligase that promotes ubiquitylation and internalization of Delta. PMID: 12530964

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm. Cell membrane. Note=Localizes to the plasma membrane.
  • 數(shù)據(jù)庫鏈接：