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  4. Recombinant Drosophila melanogaster Lissencephaly-1 homolog (Lis-1)

Recombinant Drosophila melanogaster Lissencephaly-1 homolog (Lis-1)

  • 中文名稱：
    黑腹果蠅Lis-1重組蛋白
  • 貨號(hào)：
    CSB-YP759065DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Lis-1重組蛋白
  • 貨號(hào)：
    CSB-EP759065DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Lis-1重組蛋白
  • 貨號(hào)：
    CSB-EP759065DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Lis-1重組蛋白
  • 貨號(hào)：
    CSB-BP759065DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Lis-1重組蛋白
  • 貨號(hào)：
    CSB-MP759065DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Lis-1
  • Uniprot No.：
  • 別名：
    Lis-1; Lis1; CG8440; Lissencephaly-1 homolog; DLis-1; Dlis1; Lissencephaly1
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-411
  • 氨基酸序列
    MKMVLSQRQR EELNQAIADY LGSNGYADSL ETFRKEADLS TEVEKKFGGL LEKKWTSVIR LQKKVMELEA KLTEAEKEVI EGAPTKNKRT PGEWIPRPPE KFSLTGHRAS ITRVIFHPIF ALMVSASEDA TIRIWDFETG EYERSLKGHT DSVQDVAFDA QGKLLASCSA DLSIKLWDFQ QSYECIKTMH GHDHNVSSVA FVPAGDYVLS ASRDRTIKMW EVATGYCVKT YTGHREWVRM VRVHIEGSIF ATCSNDQTIR VWLTNSKDCK VELRDHEHTV ECIAWAPEAA ASAINEAAGA DNKKGHHQGP FLASGSRDKT IRIWDVSVGL CLLTLSGHDN WVRGLAFHPG GKYLVSASDD KTIRVWDLRN KRCMKTLYAH QHFCTSIDFH KAHPYVISGS VDQTVKVWEC R
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as nuclear migration during cell division, mitotic spindle formation and the removal of mitotic checkpoint proteins from kinetochores at the metaphase to anaphase transition. Required for several aspects of neurogenesis including neuroblast proliferation, neuronal cell differentiation, dendritic growth, branching and maturation and axonal transport. Required for synchronized cell divisions in the germline, fusome integrity and oocyte differentiation. Acts together with BicD, Egl, dynein and microtubules to determine oocyte identity during oogenesis. Also required for nurse cell to oocyte transport during oocyte growth and for the positioning of the nucleus in the oocyte.
  • 基因功能參考文獻(xiàn)：
    1. lowering levels of Lissencephaly-1 (Lis1), a dynein motor co-factor, augments axonal mitochondrial transport in ageing wing neurons. PMID: 26598558
    2. The data reveal a critical role for Lis1 within the mRNA localization machinery and suggest a model in which Lis1 facilitates motor complex association with cargos by promoting the interaction of dynein with dynactin. PMID: 23918939
    3. A general role of Lis-1/NudE, Dynein and microtubules, in cell-on-cell migration, is reported. PMID: 22808215
    4. present a model in which Lis-1 and asun cooperate to regulate dynein localization and centrosome positioning during spermatogenesis PMID: 22764052
    5. Lis1, which is widely expressed in adult mammalian tissues, could regulate adult tissue stem cells through modulating niche signaling and adhesion. PMID: 21041636
    6. We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release. PMID: 16107559
    7. Mutants in the Lis1/dynactin complex strongly decrease maximum and average spindle velocity, consistent with this motor complex mediating spindle/cortex forces. PMID: 18485341

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Note=Localizes to the plus end of microtubules. Also localizes to the mitotic spindle poles from late prophase through to telophase and to kinetochore microtubules in metaphase. Localization to kinetochore microtubules is reduced in anaphase and telophase. In oocytes, it concentrates in the cortex from stage 5 of oogenesis.
  • 蛋白家族：
    WD repeat LIS1/nudF family
  • 組織特異性：
    Expressed primarily in germline cells during oogenesis. Low levels are detected in the germarium in regions 1 and 2 and in both nurse cells and the oocyte in stage 2-4 egg chambers. Enriched in the oocyte during stages 5-7 and in nurse cells in stage 8-10
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG8440

    STRING: 7227.FBpp0086375

    UniGene: Dm.646