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  4. Recombinant Drosophila melanogaster Protein patched (ptc), partial

Recombinant Drosophila melanogaster Protein patched (ptc), partial

  • 中文名稱：
    黑腹果蠅ptc重組蛋白
  • 貨號：
    CSB-YP322442DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅ptc重組蛋白
  • 貨號：
    CSB-EP322442DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅ptc重組蛋白
  • 貨號：
    CSB-EP322442DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅ptc重組蛋白
  • 貨號：
    CSB-BP322442DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅ptc重組蛋白
  • 貨號：
    CSB-MP322442DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ptc
  • Uniprot No.：
  • 別名：
    ptc; CG2411; Protein patched; Hedgehog receptor
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Segmentation polarity protein. Acts as a receptor for the hedgehog protein (HH). Associates with the smoothened protein (SMO) to transduce the hedgehog signal leading to the activation of wingless, decapentaplegic and patched itself. Participates in cell interactions that establish pattern within the segment and the imaginal disks during development. In the absence of HH, represses the constitutive signaling activity of smo through fused (FU).
  • 基因功能參考文獻：
    1. a mutation of the PTC PY motif, which mediates binding of C2-WW-HECT family members, inhibits its internalization but not its autonomous and non-autonomous signalling activities. the two related UB-C2-WW-HECT ligases NEDD4 and SU(DX) regulate PTC trafficking and finely tune its accumulation through partially redundant but distinct functions PMID: 26446620
    2. Kto-Skd affects the A/P-axial development of the whole wing disc. Kto can interact with Cubitus interruptus (Ci), bind to the Ci-binding region on ptc promoter, which are both regulated by Hh signals to down-regulate ptc expression. PMID: 24962581
    3. in vivo study demonstrates that the PI3K-cIII complex is essential for downregulating Patched via the endosomal-lysosomal pathway to execute axon pruning PMID: 25458013
    4. Self-induced patched receptor down-regulation modulates cell sensitivity to the hedgehog morphogen gradient. PMID: 20736483
    5. hedgehog (Hh) promotes the entry of Sxl into the nucleus in the wing disc; in the anterior compartment, Patched (Ptc) is required for this effect, revealing Ptc as a positive effector of Hh PMID: 14597576
    6. finding suggests that dominant-negative function can be conferred by interruption of different aspects of Patched protein behaviour PMID: 15042702
    7. Patched (Ptc), the Hh receptor, restrains both Hedgehog spreading and Hedgehog signaling. PMID: 15102702
    8. results support a model in which the ratio of bound to unbound Ptc molecules determines the cellular response to Hedgehog PMID: 15300262
    9. the Hh/Ptc complex is internalized in the Rab7-enriched lysosomal compartment in a Ptc-dependent manner without the co-receptor Smoothened (Smo). PMID: 15572139
    10. The role of Patched (Ptc) in regulating the guidance of medial tract, one of the longitudinal tracts in the nerve cord, is investigated. PMID: 15754211
    11. Ptc, as well as truncated Ptc deleted of its C-terminal domain, forms a stable trimer. This observation is consistent with the proposal that Ptc is structurally similar to trimeric transporters. PMID: 16980583
    12. lipophorins internalization does not play a major role in Hedgehog (Hh) signal transduction but does in Hh gradient formation PMID: 18198278

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  • 亞細胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Patched family
  • 數(shù)據(jù)庫鏈接：