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  4. Recombinant Enterobacteria phage lambda Large terminase protein (A), partial

Recombinant Enterobacteria phage lambda Large terminase protein (A), partial

  • 中文名稱：
    Recombinant Enterobacteria phage lambda Large terminase protein(A) ,partial,Yeast
  • 貨號：
    CSB-YP361130ECW
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Large terminase protein(A) ,partial,Yeast
  • 貨號：
    CSB-EP361130ECW
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Large terminase protein(A) ,partial,Yeast
  • 貨號：
    CSB-EP361130ECW-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Large terminase protein(A) ,partial,Yeast
  • 貨號：
    CSB-BP361130ECW
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Enterobacteria phage lambda Large terminase protein(A) ,partial,Yeast
  • 貨號：
    CSB-MP361130ECW
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    A
  • Uniprot No.：
  • 別名：
    A; lambdap02Terminase; large subunit; DNA-packaging protein A; Large terminase protein; gpA) [Includes: Endonuclease; EC 3.1.21.4); Helicase; EC 3.6.4.12); ATPase; EC 3.6.4.-)]
  • 種屬：
    Enterobacteria phage lambda (Bacteriophage lambda)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome from the concetamer to initiate and to end the packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (binding to packaging sequence cos), and a large terminase subunit possessing endonucleolytic, ATPase and helicase activities (DNA maturation and packaging). The terminase binds cooperatively with the host factor IHFA/IHFB to the cos site at the junction of adjacent viral genomes. The endonuclease activity cleaves the viral DNA generating 5'overhangs of 12 bp in length. The helicase activity separates the cohesive ends generating the single-stranded 'sticky' ends of the mature genome. IHFA/IHFB is also necessary for the strand separation activity of the terminase. The terminase remains bound to the left end of the genome to be packaged, forming a stable DNA-terminase complex. In a reaction facilitated by the viral assembly catalyst gpFI, the DNA-terminase complex binds to the portal of the procapsid thereby activating the translocase activity of the terminase. The terminase packages the viral DNA into the procapsid until the next cos site on the concatemer reaches the complex. The downstream cos site is then cut generating the mature right end of the genome, the heterotrimer undocks from the DNA-filled head and remains bound to the left end of concatemer's next genome.
  • 基因功能參考文獻：
    1. In sum, bp -2 to 5 are critical for cosN function, and bp 6-12 of the cohesive end sequence are not critical for terminase recognition or virus fitness. PMID: 28646648
    2. The authors have been able to show that the terminase motor of phage lambda is able to produce infectious particles with up to 110% of the wild-type lambda-DNA length. PMID: 22108169
  • 亞細胞定位：
    Host cytoplasm.
  • 蛋白家族：
    Lambdalikevirus large terminase family
  • 數(shù)據庫鏈接：

    KEGG: vg:2703524