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  4. Recombinant Enterococcus faecalis tRNA (Ile)-lysidine synthase (tilS)

Recombinant Enterococcus faecalis tRNA (Ile)-lysidine synthase (tilS)

  • 中文名稱：
    糞腸球菌tilS重組蛋白
  • 貨號：
    CSB-YP774377ELW
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    糞腸球菌tilS重組蛋白
  • 貨號：
    CSB-EP774377ELW
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    糞腸球菌tilS重組蛋白
  • 貨號：
    CSB-EP774377ELW-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    糞腸球菌tilS重組蛋白
  • 貨號：
    CSB-BP774377ELW
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    糞腸球菌tilS重組蛋白
  • 貨號：
    CSB-MP774377ELW
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tilS
  • Uniprot No.：
  • 別名：
    tilS; EF_0263; tRNA(Ile)-lysidine synthase; EC 6.3.4.19; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase
  • 種屬：
    Enterococcus faecalis (strain ATCC 700802 / V583)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-462
  • 氨基酸序列
    MFWQFYKRGK EQGFWQPHQT IVVAVSGGVD SMALLTLMEQ VAEKEQLQLV VAHVNHQLRE ASAQEAQYLA TYCQQRELTY YETRWEDPEK QRNLEAKART FRYEFFKEVM EIEGAAVLMT AHHLDDQAET ILMKLIRGTN FSHSAGIKER RPFATGELIR PLLIYPKEEL YQFAQRQAFV YFEDETNQTN EYLRNRLRNQ VLPLLKQENP QFLDQIASFS NEQRFAQEFI QEQIEPQLSE AVEPTKQGWR IPLKRLLKET PAYQHFFLTA FFQKTLVPLG VSLNQRQMTQ ILKVLNDERQ PQGSVMLEQQ WQLAKSYDWL CLEQKQAALR EEVTHLLVPG AGIYLSETEW LGLIATDKPF PLPEEINQWT GQLLAIPLTT ATPLTVRHRQ SGDRITLKPG FTKKLSRVFI DQKVPNEARE SAWVITDEQE EIIWVPKFAN SYLSIPLETD KIHYRLLFKT KE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRNA(Ile)-lysidine synthase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: efa:EF0263

    STRING: 226185.EF0263