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  4. Recombinant Escherichia coli DNA topoisomerase 1 (topA), partial

Recombinant Escherichia coli DNA topoisomerase 1 (topA), partial

  • 中文名稱：
    大腸桿菌topA重組蛋白
  • 貨號：
    CSB-YP024056ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌topA重組蛋白
  • 貨號：
    CSB-EP024056ENV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌topA重組蛋白
  • 貨號：
    CSB-EP024056ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌topA重組蛋白
  • 貨號：
    CSB-BP024056ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌topA重組蛋白
  • 貨號：
    CSB-MP024056ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    topA
  • Uniprot No.：
  • 別名：
    topA; supX; b1274; JW1266; DNA topoisomerase 1; EC 5.6.2.1; DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
  • 基因功能參考文獻(xiàn)：
    1. the CyaA(N600Y) mutant has a transcriptome indicative of increased CRP activity, pointing to an unknown role for CyaA and / or cAMP in gene expression..We show that the large scale transcriptomic changes in the topoisomerase I (FusA(A608E)-TopA(S180L)) mutant likely result from increased negative supercoiling in the cell. PMID: 29046437
    2. Escherichia coli RecA physically interacts with topoisomerase I to modulate the chromosomal DNA supercoiling. PMID: 27001450
    3. Structural study shows how the C-terminal domains of E. coli topoisomerase I bind single-stranded DNA to recognize the accumulation of negative supercoils in duplex DNA. PMID: 26490962
    4. Antimicrobial susceptibility and SOS-dependent increase in mutation frequency are impacted by Escherichia coli topoisomerase I C-terminal point mutation. PMID: 26248366
    5. Data indicate that transcription-coupled DNA supercoiling (TCDS) in topoisomerase I deficient (topA) is dependent on promoter strength. PMID: 23201416
    6. Topoisomerase I relaxes DNA in slow processive runs but with short pauses before runs. PMID: 22923519
    7. These data show unambiguously that Escherichia coli cells are not viable in the absence of DNA topoisomerase I without the presence of compensatory mutations. PMID: 22373098
    8. essential function is related to transcription elongation, to inhibition of R-loop formation and to allowing the synthesis of full-length mRNAs PMID: 15458416
    9. E. coli strains bearing topA mutations, introduced into the strains by DNA-mediated gene replacement, are viable at 37 or 42 degrees C without any compensatory mutations PMID: 15522872
    10. analysis of topoisomerase I DNA cleavage activity PMID: 16159875
    11. Catalytic roles of the conserved lysine and serine found at the active site of DNA topoisomerase I. PMID: 16582104
    12. in response to oxidative stress, Fis binds the promoter region of topA PMID: 17233826
    13. DNA of topA null mutants became relaxed rather than hypernegatively supercoiled following depletion of RNase HI activity. PMID: 18554330
    14. The Asp-to-Asn substitutions at the TOPRIM domain residues can selectively decrease Mg(2+) binding affinity with minimal disruption of the active-site geometry, leading to trapping of the covalent complex with cleaved DNA and causing bacterial cell death. PMID: 19013470

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  • 蛋白家族：
    Type IA topoisomerase family
  • 數(shù)據(jù)庫鏈接：