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  4. Recombinant Escherichia coli RNA polymerase sigma factor RpoH (rpoH)

Recombinant Escherichia coli RNA polymerase sigma factor RpoH (rpoH)

In Stock
  • 中文名稱：
    大腸桿菌rpoH重組蛋白
  • 貨號：
    CSB-EP313777ENV
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    rpoH
  • Uniprot No.：
  • 別名：
    rpoH; fam; hin; htpR; b3461; JW3426; RNA polymerase sigma factor RpoH; Heat shock regulatory protein F33.4; RNA polymerase sigma-32 factor
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    39.9 kDa
  • 表達區(qū)域：
    1-284aa
  • 氨基酸序列
    MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYHGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer，50% glycerol
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. Intracellular concentration of free RpoH protein increases in response to heat shock, which causes association with RNA polymerase (RNAP) and initiation of transcription of heat shock genes, including numerous global transcriptional regulators and genes involved in maintaining membrane functionality and homeostasis. RpoH is then quickly degraded, leading to a decrease in the rate of synthesis of heat shock proteins and shut-off of the heat shock response.
  • 基因功能參考文獻：
    1. ThiS, an ubiquitin-like protein, is covalently attached to delta(32) and at least 27 other proteins, leading to their subsequent degradation by proteases, in a similar manner to the ubiquitin-proteasome system (UPS) in eukaryotes. PMID: 25721662
    2. co-opts the signal recognition particle to regulate protein homeostasis PMID: 24358019
    3. Authors conclude that oxidative stress triggers the heat shock response in Escherichia coli sigma(32) dependently. PMID: 23585533
    4. N-terminal half of region 2.1 is required for modulating both metabolic stability and the activity of sigma32 PMID: 15516558
    5. First systematic study of the sigma 32 regulon in E. coli including sigma 32 protein level determination gives insight into the complex network regulated by sigma 32 and how it contributes physiological adaptation to changes in the external environment. PMID: 15757896
    6. response protects the cell membrane and responds to its status: Fully 25% of sigma32 regulon members reside in the membrane and alter its functionality; a disproportionate fraction of overexpressed proteins that induce the response are membrane localized PMID: 16818608
    7. Mutants identify a regulatory step downstream of chaperone binding that is required for both inactivation and degradation of sigma32. PMID: 17968012
    8. sigma32-mediated gene expression is a general response to protein insolubility in E. coli PMID: 17992580
    9. it is deduced from these data that there may be a link between sigma(32) regulon activity and cell division events PMID: 19114495
    10. The -10 region in sigma32 is a composite element required for high promoter activity. PMID: 19400791

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sigma-70 factor family, RpoH subfamily
  • 數(shù)據(jù)庫鏈接：