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  4. Recombinant Hepatitis E virus genotype 1 Capsid protein (ORF2), partial

Recombinant Hepatitis E virus genotype 1 Capsid protein (ORF2), partial

  • 中文名稱：
    Recombinant Hepatitis E virus genotype 1 Capsid protein(ORF2) ,partial,Yeast
  • 貨號：
    CSB-YP800425HDAU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Hepatitis E virus genotype 1 Capsid protein(ORF2) ,partial,Yeast
  • 貨號：
    CSB-EP800425HDAU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Hepatitis E virus genotype 1 Capsid protein(ORF2) ,partial,Yeast
  • 貨號：
    CSB-EP800425HDAU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Hepatitis E virus genotype 1 Capsid protein(ORF2) ,partial,Yeast
  • 貨號：
    CSB-BP800425HDAU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Hepatitis E virus genotype 1 Capsid protein(ORF2) ,partial,Yeast
  • 貨號：
    CSB-MP800425HDAU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ORF2
  • Uniprot No.：
  • 別名：
    ORF2; Secreted protein ORF2; Protein ORF2; pORF2
  • 種屬：
    Hepatitis E virus genotype 1 (isolate Human/China/HeBei/1987) (HEV)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry.; Forms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation. Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes.
  • 基因功能參考文獻：
    1. ORF2(S) does not block HEV cell entry but inhibits antibody-mediated neutralization. These results reveal a previously unrecognized aspect in HEV biology and shed new light on the immune evasion mechanisms and pathogenesis of this virus. PMID: 29669922
    2. findings show that the hepatitis E virus (HEV) ORF2 protein interacts directly with the ectodomain of both ASGR1 and ASGR2; ASGPR is involved in and facilitates HEV infection by binding to ORF2 PMID: 27155063
    3. Fndings indicate that specific substitutions at position 562 on ORF2 have more measurable effect on the activity of the hepatitis E virus neutralizing epitope than dimerization or glycosylation of the structural protein. PMID: 26584510
    4. Both partial ORF2 sequence identity comparisons and phylogenetic analysis based on sequencing of the partial ORF2 gene suggest that some circulating HEV strains on Canadian pork livers are more closely related to human disease producing HEV strains. PMID: 25555901
    5. Complete epitope mapping of ORF2 with the construction of multiple specific antibodies has been reported. PMID: 26085097
    6. V239A substitution within the helicase domain, which is associated with increased virulence of genotype 3 HEV. PMID: 25916609
    7. We describe an additional RNA element that is located in a central region of ORF2 taht plays an important role in one of the early steps of virus replication. PMID: 23515023
    8. Data suggest that the hydrophobic core polypeptide of capsid protein (p239; aa368-606) plays important role in protein particle assembly; p239 particle assembly rate is largely affected by ionic strength and temperature. PMID: 23281113
    9. The C-terminal 52 amino acids of hepatitis E virus capsid is essential for the viral life cycle, promoting accurate encapsidation and stabilizing encapsidated particles. PMID: 23468481
    10. ORF2 protein could directly associate with the F-box protein, beta transducin repeat containing protein (betaTRCP) and ORF2 over expression resulted in reduced association of IkappaBalpha with the SKP1 and CUL1 components of the SCFbetaTRCP complex. PMID: 22590978
    11. The results showed that the F51L mutation partially contributed to virus attenuation, whereas the T59A and S390L mutations resulted in more drastic attenuation of hepatitis E virus in pigs. PMID: 21450834
    12. mutations that eliminate potential glycosylation sites in the capsid protein (ORF2) did not detectably affect genome replication or capsid protein synthesis in cell culture; however, none of the mutants infected rhesus macaques PMID: 18032496
    13. These data suggested Leu477 and Leu613 are critical in forming the neutralization epitope of HEV genotype 4. PMID: 19118511
    14. There is at least one type-common neutralizing antigenic epitope in ORF2 encoded protein of the identified HEV genotype 4. PMID: 19428747

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  • 亞細胞定位：
    [Isoform Secreted protein ORF2]: Secreted.; [Isoform Capsid protein ORF2]: Virion. Host cytoplasm. Host endoplasmic reticulum. Host Golgi apparatus. Host cell surface.
  • 蛋白家族：
    Hepevirus capsid protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:1494410