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  4. Recombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A)

Recombinant Human S-adenosylmethionine synthase isoform type-2 (MAT2A)

In Stock
  • 中文名稱：
    人MAT2A重組蛋白
  • 貨號(hào)：
    CSB-EP013517HUc7
  • 規(guī)格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    (AdoMet synthase 2)(Methionine adenosyltransferase 2)(MAT 2)(Methionine adenosyltransferase II)(MAT-II)
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    E.coli
  • 分子量：
    44.8 kDa
  • 表達(dá)區(qū)域：
    1-395aa
  • 氨基酸序列
    MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
  • 基因功能參考文獻(xiàn)：
    1. MAT2A expression is upregulated through mRNA stabilization upon SAM depletion. PMID: 29262316
    2. Increased MAT2A expression is associated with recurrence in hepatocellular carcinoma. PMID: 29448301
    3. High MAT2A expression is associated with tamoxifen-resistant breast cancer. PMID: 26418898
    4. METTL16 that controls MAT2A intron retention in response to intracellular SAM levels; splicing of the MAT2A retained intron is rapidly induced upon Met depletion, and this effect requires a conserved hairpin which is a METTL16 m6A substrate. PMID: 28525753
    5. methionine adenosyltransferase II alpha (MAT2A), and the arginine methyltransferase, PRMT5, as vulnerable enzymes in cells with MTAP deletion. PMID: 27068473
    6. crystal structures of human MATalpha2 containing various bound ligands. PMID: 26858410
    7. acetylation decreased in hepatocellular cancer PMID: 25925782
    8. Mutagenesis of MATalpha2 and MATbeta phospho-sites destabilized them and prevented hepatic stellate cell trans-differentiation. PMID: 25294683
    9. The data presented here support the conclusion that rare genetic variants in MAT2A predispose individuals to thoracic aortic disease. PMID: 25557781
    10. Results suggest that MAT2A is downregulated in cancer tissues of renal cell carcinomas patients and has function of tumor suppressor though repressing the expression of heme oxygenase-1. PMID: 24636201
    11. Differential gene expression of BACE1 and PSEN1 in the knockdown cells, which is possibly a consequence of MAT2A deregulation and may indicate a self regulatory mechanism. PMID: 22879628
    12. hypoxia-induced MAT2A expression is HIF-1alpha dependent PMID: 21460102
    13. Insulin-like growth factor 1 (IGF1) activates methionine adenosyltransferase 2A MAT2A) transcription by both known and novel pathways in colon cancer cells. PMID: 21406062
    14. X protein of hepatitis B virus inhibits apoptosis in hepatoma cells through enhancing the methionine adenosyltransferase 2A gene expression and reducing S-adenosylmethionine production PMID: 21247894
    15. regulation of expression in hepatocarcinoma cells by L-methionine availability PMID: 12660248
    16. beta subunit associates with cirrhosis and cancer providing a proliferative advantage in hepatoma cells through its interaction with methionine adenosyltransferase II alpha2 and down-regulation of S-adenosylmethionine levels. PMID: 12671891
    17. up-regulation of MAT2A also provides a growth advantage and s-adenosylmethionine and methylthioadenosine can block mitogenic signaling in colon cancer cells PMID: 17631143
    18. Lower expression of both MAT2A and MAT2beta and interfere with leptin signaling in liver cancer cells. PMID: 18041713
    19. This work was carried out to examine the role of MAT II activity and S-adenosylmethionine biosynthesis in the survival of leukemic T cells. PMID: 19048023

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  • 蛋白家族：
    AdoMet synthase family
  • 組織特異性：
    Detected in kidney.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 6904

    OMIM: 601468

    KEGG: hsa:4144

    STRING: 9606.ENSP00000303147

    UniGene: Hs.516157