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  4. Recombinant Human 1-phosphatidylinositol 3-phosphate 5-kinase (PIKFYVE), partial

Recombinant Human 1-phosphatidylinositol 3-phosphate 5-kinase (PIKFYVE), partial

  • 中文名稱：
    人PIKFYVE重組蛋白
  • 貨號(hào)：
    CSB-YP896875HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人PIKFYVE重組蛋白
  • 貨號(hào)：
    CSB-EP896875HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人PIKFYVE重組蛋白
  • 貨號(hào)：
    CSB-EP896875HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人PIKFYVE重組蛋白
  • 貨號(hào)：
    CSB-BP896875HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人PIKFYVE重組蛋白
  • 貨號(hào)：
    CSB-MP896875HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    1 phosphatidylinositol 3 phosphate 5 kinase; 1 phosphatidylinositol 4 phosphate 5 kinase; 1-phosphatidylinositol 3-phosphate 5-kinase; CFD; epididymis luminal protein 37; FAB1; FAB1; S. cerevisiae; homolog of; FYV1_HUMAN; FYVE finger containing phosphoinositide kinase; FYVE finger-containing phosphoinositide kinase; KIAA0981; MGC40423; p235; Phosphatidylinositol 3 phosphate 5 kinase type III; Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III; Phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase type III; Phosphatidylinositol 4 phosphate 5 kinase type III; Phosphoinositide kinase; FYVE finger containing; PIKfyve; PIP5K; PIP5K3; PIPkin III; PIPkin-III; PtdIns(4)P 5 kinase; Type III PIP kinase; ZFYVE29; Zinc finger; FYVE domain containing 29
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression. The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form (PtdIns(3,5)P2). Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5-phosphate (PtdIns(5)P). Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate. Autophosphorylation inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and downregulates lipid product formation. Involved in key endosome operations such as fission and fusion in the course of endosomal cargo transport. Required for the maturation of early into late endosomes, phagosomes and lysosomes. Regulates vacuole maturation and nutrient recovery following engulfment of macromolecules, initiates the redistribution of accumulated lysosomal contents back into the endosome network. Critical regulator of the morphology, degradative activity, and protein turnover of the endolysosomal system in macrophages and platelets. In neutrophils, critical to perform chemotaxis, generate ROS, and undertake phagosome fusion with lysosomes. Plays a key role in the processing and presentation of antigens by major histocompatibility complex class II (MHC class II) mediated by CTSS. Regulates melanosome biogenesis by controlling the delivery of proteins from the endosomal compartment to the melanosome. Essential for systemic glucose homeostasis, mediates insulin-induced signals for endosome/actin remodeling in the course of GLUT4 translocation/glucose uptake activation. Supports microtubule-based endosome-to-trans-Golgi network cargo transport, through association with SPAG9 and RABEPK. Mediates EGFR trafficking to the nucleus.; (Microbial infection) Required for cell entry of coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus EMC (HCoV-EMC) by endocytosis.
  • 基因功能參考文獻(xiàn)：
    1. this study shows that PIKfyve coordinates the neutrophil immune response through the activation of the Rac GTPase PMID: 28779020
    2. in PC-3 cells inhibition of PIKfyve by apilimod or depletion by siRNA increased the secretion of the exosomal fraction. PMID: 27438886
    3. A cell-permeable tool for analysing APP intracellular domain function and manipulation of PIKfyve activity. PMID: 26934981
    4. A novel heterozygous frameshift mutation (c.3151dupA) and a copy number variations in PIKFYVE gene have been found in two unrelated Fleck corneal dystrophy patients. PMID: 26396486
    5. The PIKfyve complex is required for APP trafficking, suggesting a feedback loop in which APP, by binding to and stimulating phosphatidylinositol-3,5-bisphosphate vesicle formation may control its own trafficking. PMID: 26216398
    6. APP functionally cooperates with PIKfyve in vivo. This regulation is required for maintaining endosomal and neuronal function. PMID: 26125944
    7. data identify a novel role of the ArPIKfyve-Sac3 complex in the mechanisms controlling aggregate formation of Sph1 and suggest that Sac3 protein deficiency or overproduction may facilitate aggregation of aggregation-prone proteins PMID: 26405034
    8. Data suggest PIKFYVE, MTMR3 (myotubularin related protein 3) and their product phosphatidylinositol 5-phosphate are involved in activation of RAC1 (rho family small GTP binding protein); this process regulates migration/invasion of carcinoma/sarcoma. PMID: 24840251
    9. Data indicate that pharmacological or genetic inactivation of PIKfyve rapidly induces expression of the transcription repressor ATF3, which is necessary and sufficient for suppression of type I IFN expression. PMID: 24600036
    10. Data indicate that AKT promotes EGFR recycling by phosphorylating and activating PIKfyve. PMID: 23757022
    11. the present observations show that PKB in conjunction with PIKfyve activates Kir2.1 channels. PMID: 23188060
    12. Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. PMID: 23154468
    13. The results provide the first experimental evidence that the principal pathway for PtdIns5P intracellular production is through PIKfyve. PMID: 22621786
    14. A novel c.3060-3063 delCCTT (p.P968Vfs23) mutation in the PIKFYVE gene has been described in a five generation Greek family, which segregated with the fleck corneal dystrophy. PMID: 22065932
    15. The recent advances in Arf6/PIP5K signaling and its linkage to cellular functions, are reviewed. PMID: 20945365
    16. The first multicellular model for PIKFYVE loss, pointing to a role in lysosome maturation PMID: 16801682
    17. REVIEW : PIKFYVE and other phosphoinositides regulatory proteins are implicated in human genetic diseases PMID: 18429927
    18. A phylogenetic study revealing co-evolution of phosphoinositides kinases and phosphatases ; PIKFYVE is absent from several organisms and co-evolved with VAC14 and FIG4 PMID: 18774718
    19. Coexpression of PIKfyve is followed by a marked increase of glutamate induced currents in EAAT2 expressing oocytes. PMID: 19910676
    20. p40 interaction with PIKfyve p40 os demonstrated; PIKfyve interaction and the subsequent PIKfyve-catalyzed p40 phosphorylation anchor p40 to discrete membranes facilitating late endosome-to-TGN transport. PMID: 14530284
    21. PIKfyve selectively regulates the sorting and traffic of peripheral endosomes containing lysosomaly directed fluid phase cargo through controlling the morphogenesis and function of multivesicular bodies PMID: 14551253
    22. Linkage analysis localized CFD to a 24-cM (18-Mb) interval of chromosome 2q35 flanked by D2S2289 and D2S126 and containing PIP5K3. PMID: 15902656
    23. PIKfyve is distributed in microdomains that are distinct from those occupied by EEA1 and Hrs PMID: 16448788
    24. PIKfyve regulates endosome-to-TGN retrograde transport. PMID: 16954148
    25. results suggest that the local production of PtdIns(3)P implicates the fusion of macropinosomes via EEA1 as well as conventional early endosomes PMID: 17146146
    26. The observations disclose that PIKfyve participates in the SGK1-dependent regulation of SLC5A1. PMID: 17570343
    27. These data suggest for the first time a role of PtdIns5P and PIKfyve in oncogenesis, potentially linking intracellular trafficking to cancer. PMID: 18501703
    28. Kinesin adapter JLP links PIKfyve to microtubule-based endosome-to-trans-Golgi network traffic of furin. PMID: 19056739
    29. PIKfyve is a potent stimulator of ClC-2-activity and contributes to SGK1-dependent regulation of ClC-2. PMID: 19232516
    30. Results suggest that PIKfyve inhibition may render the late endosome/lysosome compartment refractory to fusion with both autophagosomes and with EGFR-containing multivesicular bodies. PMID: 19582903
    31. PIKfyve-dependent channel degradation is essential to prevent Ca2+-induced toxicity in neurons. PMID: 19841139
    32. these results demonstrate that PIKfyve regulates CFTR activity, and suggest a novel mechanism of CFTR regulation. PMID: 19852935

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  • 相關(guān)疾?。?/div>
    Corneal dystrophy, fleck (CFD)
  • 亞細(xì)胞定位：
    Endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 23785

    OMIM: 121850

    KEGG: hsa:200576

    STRING: 9606.ENSP00000264380

    UniGene: Hs.744997