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Recombinant Human Angiopoietin-like protein 8 (ANGPTL8)

  • 中文名稱：
    Recombinant Human Angiopoietin-like protein 8(ANGPTL8),Yeast
  • 貨號：
    CSB-YP757793HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human Angiopoietin-like protein 8(ANGPTL8),Yeast
  • 貨號：
    CSB-EP757793HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ANGPTL8; C19orf80; RIFL; UNQ599/PRO1185Angiopoietin-like protein 8; Betatrophin; Lipasin; Refeeding-induced fat and liver protein
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    22-198
  • 氨基酸序列
    APMGGPELA QHEELTLLFH GTLQLGQALN GVYRTTEGRL TKARNSLGLY GRTIELLGQE VSRGRDAAQE LRASLLETQM EEDILQLQAE ATAEVLGEVA QAQKVLRDSV QRLEVQLRSA WLGPAYREFE VLKAHADKQS HILWALTGHV QRQRREMVAQ QHRLRQIQER LHTAALPA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

We would like to know the protein concentration and cell strain of CSB-MP757793HU.
https://www.cusabio.com/Recombinant-Protein/Recombinant-Mouse-Hepatocellular-carcinoma-associated-protein-TD26-homologGm648-Mammalian-cell-12547434.html#a01
We are looking forward to hearing from you.

A:
Recombinant Human Angiopoietin-like protein 8(ANGPTL8)
CSB-MP757793HU >> Mammalian cell
Expression Region: 22-198aa; Full length of the mature protein.
Tag information:Tag type will be determined during the manufacturing process.
We can try your desired tag preferentially if you have, but as we can't 100% guarantee, if it's failed, we will provide the protein with other tag.
The success rate of expressing the protein with your desired tag is 95.25%.
Target protein sequence: (The whole complete sequence will include tag sequene, target protein sequence and any linker sequence)

APMGGPELAQHEELTLLFHGTLQLGQALNGVYRTTEGRLTKARNSLGLYGRTIELLGQEVSRGRDAAQELRASLLETQMEEDILQLQAEATAEVLGEVAQAQKVLRDSVQRLEVQLRSAWLGPAYREFEVLKAHADKQSHILWALTGHVQRQRREMVAQQHRLRQIQERLHTAALPA


Generally, we use HEK293 for the Mammalian cell expression system, and the concentration ranges from 0.1mg/ml to 0.5mg/ml.

靶點詳情

  • 功能：
    Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels. May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state.
  • 基因功能參考文獻：
    1. ANGPTL8 levels are lower in Prader-Willi syndrome than obese controls and are inversely associated with the severity of liver steatosis. Further studies should investigate the potential genetic basis for this observation. PMID: 28600576
    2. Inhibition of miR-143-3p amplified ANGPTL8 response to treatments (glucose, insulin, Lipopolysaccharides), suggesting that the miRNA acts to suppress ANGPTL8 expression under metabolically distorted conditions. PMID: 30261196
    3. Study suggests that ANGPTL8 levels in early pregnancy are significantly and independently associated with risk of GDM at 24-28 weeks of gestation. PMID: 29167926
    4. Circulating full-length ANGPTL8 concentrations in patients with dyslipidemia were significantly elevated as compared to controls. PMID: 30021605
    5. ApoCIII may mediate the effects of ANGPTL8 on triglyceride metabolism. PMID: 30021607
    6. Study shows that angiopoietin-like protein 8 (ANGPTL-8) was positively correlated with hepatocellular lipid content independent of obesity and insulin resistance, indicating that ANGPTL-8 might be a new and important important predictor of the severity of non-alcoholic fatty liver disease. PMID: 29266821
    7. We conclude that serum betatrophin concentrations were increased in pregnancies affected by hyperemesis gravidarium. PMID: 29754072
    8. Data suggest the angiopoietin-like 8 (ANGPTL8)/p62-IKKgamma axis as a negative feedback loop that regulates NF-kappaB activation, and extends the role of selective autophagy in fine-tuned inflammatory responses. PMID: 29255244
    9. ANGPTL8 levels are increased in both plasma and adipose tissues of subjects with hypertension. PMID: 29490644
    10. Serum ANGPTL8 concentrations were significantly increased in impaired glucose regulation and type 2 diabetes. PMID: 29082263
    11. the level of circulating betatrophin is positively associated with insulin resistance. PMID: 28931172
    12. we identified ANGPTL8 as the target gene at this HDL-C GWAS locus, determined regulatory drivers of tissue specificity, and combined fine-mapping approaches and regulatory overlap with experimental assays to identify variants that may contribute to the HDL-C GWAS signal at ANGPTL8. PMID: 28754724
    13. serum levels not associated with weight status, glucose tolerance, insulin resistance, or lipid metabolism PMID: 27402552
    14. Elevated betatrophin levels in polycystic ovary syndrome women, in the absence of obesity and glucose intolerance, may reflect a compensatory mechanism in order to counteract metabolic syndrome-related risk factors. PMID: 28319674
    15. betatrophin levels appeared to be related to the pathogenesis of the diabetic stages rather than prediabetic stages. PMID: 28351091
    16. Angiopoietin-like protein 8/betatrophin is related to liver steatosis, while visceral adipose tissue represents an additional site of expression in humans. PMID: 28351093
    17. ANGPTL8 reverses established Adriamycin induced cardiomyopathy by stimulating adult cardiac progenitor cells. PMID: 27823982
    18. Study provided an update to understand its physiological function from literature curated findings through an integrated view of ANGPTL8's regulation and its associated pathways. PMID: 28684091
    19. betatrophin is increased in pancreatic cancer-associated diabetes PMID: 27276680
    20. These results confirm a role for ANGPTL8 in lipoprotein metabolism and provide novel support for functional consequences of the rs2278426 variant. PMID: 27117576
    21. ANGPTL8 has a functional LPL inhibitory motif, but only inhibits LPL and increases plasma TG levels in mice in the presence of ANGPTL3 PMID: 28413163
    22. The inflammation-induced miR-221-3p regulates ANGPTL8 expression in adipocytes. This miRNA impact may become especially prominent under pathologic conditions such as morbid obesity, putatively contributing to the impaired adipose tissue lipid metabolism in metabolic disease. PMID: 28938482
    23. Circulating ANGPTL8 increases after bariatric surgery and predicts type 2 diabetes remission in morbidly obese patients. PMID: 28347650
    24. Data suggest that plasma betatrophin levels are significantly increased in lean (normal-weight) women with PCOS (polycystic ovary syndrome) compared to lean women without PCOS; plasma betatrophin levels are not significantly altered in overweight/obese women with PCOS compared to overweight/obese women without PCOS. This study was conducted in China. PMID: 27960599
    25. Especially the association to eGFR highlights the importance for future studies to address renal function as possible influence on betatrophin regulation and consider eGFR as potential confounder when analyzing the role of betatrophin in humans PMID: 28257453
    26. Serum betatrophin is an independent risk factor for nonalcoholic fatty liver and potential non-invasive marker for its progression. PMID: 28125672
    27. Cord blood betatrophin may function as a potential biomarker of maternal intrauterine hyperglycemia and fetal insulin resistance, which may presage for long-term metabolic impact of gestational diabetes on offspring PMID: 27196053
    28. The high levels of ANGPTL8 found in fetal life together with its relationship with newborn adiposity and brown adipose tissue point to ANGPTL8 as a potential new player in the modulation of the thermogenic machinery during the fetal-neonatal transition. PMID: 27469268
    29. Betatrophin levels were increased in women with PCOS and were associated with insulin resistance, c-reactive protein, and free-testosterone in these patients. PMID: 26832343
    30. Obesity is associated with increased betatrophin suppression after an oral glucose load; this response appears to be driven by hyperglycemia. Data suggest that the impaired betatrophin response in obese subjects is restored after weight loss and is comparable with the betatrophin response in lean individuals. PMID: 27459526
    31. Serum betatrophin levels are increased and associated with insulin resistance in patients with polycystic ovary syndrome. PMID: 28222635
    32. ANGPTL8/betatrophin might play an important role in glucose metabolism in the context of insulin resistance. PMID: 26387753
    33. This study supports that serum betatrophin plays an important role in MetS, involving the regulations of glucose and lipid metabolism and inflammation. PMID: 27238790
    34. our data shows that ANGPTL3, 4 and 8 are increased in obesity and type 2 diabetes (T2D). ANGPTL8 associates with ANGPTL3 in the non-diabetic subjects while it associated more with ANGPTL4 in the obese and T2D subjects. PMID: 27733177
    35. circulating betatrophin is increased in mice and humans with NAFLD and its expression was induced by endoplasmic reticulum stress in hepatocytes PMID: 27045862
    36. High betatrophin expression is associated with diabetic metabolic syndrome. PMID: 27578619
    37. Elevated betatrophin levels were associated with cardiometabolic risk factors a young population, but the association was largely dependent on vitamin D status. PMID: 27716289
    38. betatrophin may be associated with thyroid insufficiency but not thyroid autoimmunity. PMID: 27213151
    39. Betatrophin levels in patients with polycystic ovary syndrome (PCOS) are lower than those without PCOS and inversely related to insulin resistance. PMID: 27188865
    40. data shows for the first time that heterozygote form of ANGPTL8 Rs.2278426 variant was associated with higher FBG level in Arabs highlighting the importance of these variants in controlling the function of betatrophin. PMID: 26864934
    41. Serum betatrophin concentrations are increased in type 2 diabetes patients under antidiabetic treatment and positively associated with diabetic retinopathy. PMID: 26863068
    42. Compared with type 2 diabetes without cardiovascular disease (CVD), patients with CVD had remarkably higher levels of angiopoietin-like protein 8 (ANGPTL8). PMID: 27596060
    43. Circulating betatrophin concentration is negatively correlated with insulin resistance in obese children and adolescents. PMID: 27103367
    44. Meta-analysis results from case control studies on betatrophin and type 2 diabetes (T2DM) revealed increased circulating levels of betatrophin in patients with T2DM. PMID: 27242389
    45. ANGPTL8 levels were associated with baseline and future changes in triglyceride levels in Korean children. PMID: 26739706
    46. Betatrophin is significantly increased in T2DM patients with different stages of albuminuria. Betatrophin may be a novel endocrine regulator involved in DN development. PMID: 26739836
    47. ANGPTL8 is increased in subjects with MetS and it was significantly associated with HsCRP levels in different subgroups. PMID: 26850725
    48. Disruption of ANGPTL8 function in humans does not seem to have a large effect on measures of glucose tolerance. PMID: 26822414
    49. Based on the findings of our meta-analysis, circulating betatrophin level of T2DM patients is higher than that of nondiabetic adults in the nonobese population, but not in the obese population. PMID: 26697500
    50. In patients with diabetes mellitus type 2, betatrophin levels did not correlate with beta-cell function-related variables or insulin resistance-related variables PMID: 26649318

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  • 相關疾病：
    Diabetes mellitus, insulin-dependent (IDDM); Diabetes mellitus, non-insulin-dependent (NIDDM)
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ANGPTL8 family
  • 組織特異性：
    Predominantly expressed in liver. Also expressed in adipose tissues.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 24933

    OMIM: 125853

    KEGG: hsa:55908

    STRING: 9606.ENSP00000252453

    UniGene: Hs.534467