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Recombinant Human Aromatase (CYP19A1)

In Stock
  • 中文名稱：
  • 貨號(hào)：
    CSB-EP006394HUa0
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    CYP19A1
  • Uniprot No.：
  • 別名：
    CYPXIX;Cytochrome P-450AROM;Cytochrome P450 19A11 Publication;Estrogen synthase
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    E.coli
  • 分子量：
    62.0 kDa
  • 表達(dá)區(qū)域：
    1-503aa
  • 氨基酸序列
    MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGIGSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPELWKTTRPFFMKALSGPGLVRMVTVCAESLKTHLDRLEEVTNESGYVDVLTLLRRVMLDTSNTLFLRIPLDESAIVVKIQGYFDAWQALLIKPDIFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFATELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDIQKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAKNVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEMIFTPRNSDRCLEH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能參考文獻(xiàn)：
    1. Polymorphism in CYP19A1 is not associated with polycystic ovary syndrome. PMID: 29564739
    2. CYP19A1 was the only gene harboring single nucleotide polymorphisms that were significantly associated with prostate cancer risk PMID: 29228205
    3. CYP19A1 mRNA expression and aromatase activity during syncytialization of primary human villous trophoblast cells at term PMID: 29474975
    4. in postmenopausal women, aromatase activity could be an important determinant of skeletal health. PMID: 29329467
    5. Fungicide ziram may disrupt the placental steroid production. In the present study, we investigated the effects of ziram on steroid formation in human placental cell line JEG-3 cells and on HSD3B1 and aromatase in the human placenta. Ziram did not inhibit progesterone production in JEG-3 cells and HSD3B1 activity at 100muM... ziram is a potent inhibitor of human aromatase. PMID: 28951168
    6. This study demonstrated the existence of an AMH-FOXL2 relationship in hGCs. AMH is capable of increasing both gene and protein expression of FOXL2. Because FOXL2 induces AMH transcription, these ovarian factors could be finely regulated by a positive feedback loop mechanism to preserve the ovarian follicle reserve. PMID: 28660501
    7. The present study reports on the synthesis of pituitary aromatase, its regulation by gonadal steroids, and the physiological roles of aromatase on pituitary endocrine cells. The involvement of aromatase in the pathogenesis of pituitary tumors, mainly prolactinomas, through the auto-paracrine production of estradiol is reviewed. [review] PMID: 29104246
    8. CYP19A1 Val80, a genetic polymorphism related to the conversion of testosterone into estrogen, significantly contributes to individual differences in self-construal and subjective well-being. PMID: 28888137
    9. The findings indicate that the CYP19 alleles rs727479A/C and rs700519C/T might be associated with the pregnancy outcome after assisted reproductive technology in patients with polycystic ovary syndrome. PMID: 29050673
    10. significant prognostic roles of ERalpha, ERbeta and aromatase were discovered in the in prostate cancer specimens of our large multicenter cohort. PMID: 27610593
    11. These studies suggest an association between adiposity, aromatase estrogenic capacity and atrial arrhythmogenicity. PMID: 28822806
    12. Changes in the CYP19A1 expression in adipose tissue stromal vascular fraction cells, induced by free acids, correlated positively with BMI of patients but only in group of obese or overweight women. PMID: 29629743
    13. In conclusion, activated Smad3 proteins can bind to P450arom promoter -705/+87 bp region, responsive to activin A in endometrial stromal cells, which can promote P450arom transcription. PMID: 27920344
    14. polymorphisms not associated with late miscarriage PMID: 28410957
    15. this study illustrated that the brain-specific alternate promoter PI.f derived CYP19 expression can be regulated by DNA methylation. PMID: 28559115
    16. sex steroid hormones and sex hormone binding globulin levels, CYP17 MSP AI (-34T:C) and CYP19 codon 39 (Trp:Arg) variants in children with developmental stuttering PMID: 28992603
    17. RET inhibitors could both impair primary tumor growth and tumor dissemination, thereby providing a potential therapeutic advantage when used in combination with aromatase inhibitors in postmenopausal ER+ breast cancers. PMID: 27602955
    18. We suggest from these studies that overexpression of aromatase in breast tissue and its resultant increase in estradiol levels may contribute to the later development of breast cancer in women with atypical ductal hyperplasia PMID: 28337664
    19. As regards the CYP19A1 variant, genotype 11 (del/del) was more frequent in patients than in controls (OR=1.85; 95% CI 1.06-3.22; P=0.03). PMID: 27145040
    20. C-Jun and CREB are recruited to ESR2 or CYP19A1 promoter by IGF-I stimulation. PMID: 26899323
    21. The results of the present study suggest that AhR stimulates estrogen-dependent progression of breast carcinoma by inducing aromatase expression under some conditions. These results provide new insights on the possible roles of environmental toxins in breast cancer development. PMID: 27900579
    22. In the postmortem study, the amount of PVN aromatase immunoreactivity decreased significantly in the major depressive disorder group compared to the controls PMID: 28024269
    23. Results show that aromatase protein expression is increased in bladder neoplasm tissue and may be associated with advanced tumor stage. PMID: 27324053
    24. Polymorphism of CYP19 is associated with breast cancer. PMID: 28178648
    25. The combination of high carbohydrate intake and the heterozygote (CG) of CYP19A1 rs4441215 showed a higher serum estradiol level in postmenopausal Japanese women. PMID: 28980890
    26. Leptin increased aromatase expression in breast cancer cells, which was correlated with COX-2 upregulation. PMID: 28571770
    27. Immunohistochemical analysis of 221 invasive breast cancer cases indicated that 87.3% (193/221) had at least 5% aromatase positive cells. PMID: 28489882
    28. CYP19A1 amplification caused increased aromatase activity. PMID: 28112739
    29. The reduction of DHT obese homozygotic twins could be linked to its increased degradation by AKR1C2 and HSD11B1, and increased estrogen levels could be linked to increased adiposity-related expression of aromatase in adipose tissue. PMID: 28619249
    30. The authors proposed that NF2 behaves as a protein sensing tissue damage and aromatase-driven local estrogen formation, eventually leading to regulation of stem cells differentiation and tissue repair by liver cancer cells. (Review) PMID: 27289045
    31. The CYP19A1 rs10046 variant T/T favors lower incidence of hot flashes/sweating under exemestane + OFS treatment, suggesting endocrine-mediated effects. PMID: 27825388
    32. R264 polymorphism causes an intrinsic alteration of aromatase activity together with a different consensus for phosphorylation by different kinases, indicating that estrogen production can be different when such mutations are present PMID: 27702664
    33. analysis of how sildenafil inhibits aromatase in human cells by molecular docking simulation PMID: 27616271
    34. 16(beta)-OH-androstenedione generated by CYP21A2 serves as a substrate for CYP19A1 PMID: 28065637
    35. Description of a novel functional FSHR expression, where FSH-stimulated CYP19A1 expression and estrogen production in recto-vaginal endometriotic nodules (RVEN) are demonstrated. This locally FSH-induced estrogen production may contribute to the pathology, development, progression, and severity of RVEN . PMID: 27224263
    36. Estradiol synthesis in the hippocampus is regulated by several mechanisms, which are reviewed in this report. The regulation of the activity of aromatase, the final enzyme of estrogen biosynthesis, by Ca(2+) transients, is of particular interest. [review] PMID: 26472556
    37. CYP19A1 activity may contribute to disordered steroidogenesis in female patients with POR mutations as well as in mothers with POR variants carrying a male child PMID: 27032764
    38. The structural and functional importance of estrogen receptor and aromatase in endocrine-responsive and -resistant breast cancers will be discussed in more detail. [review] PMID: 26277097
    39. Aromatase inhibitor treatment significantly increased expression of let-7f in Ishikawa cells and endometrial stroma cells from patients with endometriosis. PMID: 27320036
    40. results show that the Aha1-Hsp90-PKM2/HIF-1alpha axis mediates the induction of aromatase in Li-Fraumeni Syndrome. PMID: 27467582
    41. Data show that the combination of nobiletin (NOB), hesperetin (HES) and letrozole (LET) had no effects on activity and expression of aromatase (cytochrome P450 CYP19) in MCF-7 breast cancer cells. PMID: 28364779
    42. The expression of P450 aromatase corresponds to specific cell morphology in sex cord-stromal tumors, including recurrent tumors. PMID: 26166720
    43. Association study investigated the ESR1, ESR2, and CYP19A1 gene polymorphisms in Chinese migraine patients, data suggested that ESR1 rs2234693 plays a potential role in migraine susceptibility in a Chinese population, especially for menstrually related migraine PMID: 27778160
    44. A novel 568C insertion mutation in CYP19A1 can lead to severe aromatase deficiency. Homozygosity for this mutation is associated with the development of hypoplastic ovaries PMID: 27086564
    45. Significant reduction of CYP19A1 gene expression in Cumulus cells of endometriosis patients may be the result of epigenetic alterations in its regulatory regions, either by DNA methylation or histone modifications. PMID: 27167072
    46. In the bone marrow, cyclin A1 and aromatase enhanced local bone marrow-releasing factors, including androgen receptor, estrogen and matrix metalloproteinase MMP9 and promoted the metastatic growth of prostate cancer cells PMID: 26921336
    47. No association of functional polymorphisms in the estrogen metabolism genes CYP19A1 with menstrual migraine. PMID: 28089731
    48. The results demonstrated that the rs3751592 A/G polymorphism of the CYP19A1 gene was associated with the incidence of Alzheimer disease in a Chinese Han population, which suggests that CYP19A1 rs3751592 is a predisposing genetic factor for Alzheimer disease. PMID: 27583919
    49. CYP19 Gene Polymorphism is associated with Breast Cancer. PMID: 27165202
    50. The data showed that the effect of Activin A on ESCs was partially abrogated by pretreatment with an inhibitor of ALK4 (the type I receptor, ActRIB) and Smad4-siRNA. Cumulatively, these data suggest that Activin A promotes the secretion of estradiol from ESCs by increasing the expression of P450arom via the ALK4-Smad pathway. These findings indicate the ALK4-Smad pathway may promote ectopic lesion survival and developmen PMID: 27833918

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  • 相關(guān)疾?。?/div>
    Aromatase excess syndrome (AEXS); Aromatase deficiency (AROD)
  • 亞細(xì)胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 組織特異性：
    Widely expressed, including in adult and fetal brain, placenta, skin fibroblasts, adipose tissue and gonads.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2594

    OMIM: 107910

    KEGG: hsa:1588

    STRING: 9606.ENSP00000260433

    UniGene: Hs.260074