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  4. Recombinant Human BRCA1-A complex subunit RAP80 (UIMC1)

Recombinant Human BRCA1-A complex subunit RAP80 (UIMC1)

  • 中文名稱：
    人UIMC1重組蛋白
  • 貨號：
    CSB-YP857019HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人UIMC1重組蛋白
  • 貨號：
    CSB-EP857019HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人UIMC1重組蛋白
  • 貨號：
    CSB-EP857019HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人UIMC1重組蛋白
  • 貨號：
    CSB-BP857019HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人UIMC1重組蛋白
  • 貨號：
    CSB-MP857019HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    UIMC1
  • Uniprot No.：
  • 別名：
    BRCA1-A complex subunit RAP80; Nuclear zinc finger protein RAP80; OTTHUMP00000161441; OTTHUMP00000223372; OTTHUMP00000223374; RAP80; Receptor associated protein 80; Receptor-associated protein 80; Retinoid X receptor interacting protein 110; Retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RIP110; Rxrip110; Ubiquitin interaction motif containing 1; Ubiquitin interaction motif containing protein 1; Ubiquitin interaction motif-containing protein 1; UIMC1; UIMC1_HUMAN; X2HRIP110
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-719
  • 氨基酸序列
    MPRRKKKVKE VSESRNLEKK DVETTSSVSV KRKRRLEDAF IVISDSDGEE PKEENGLQKT KTKQSNRAKC LAKRKIAQMT EEEQFALALK MSEQEAREVN SQEEEEEELL RKAIAESLNS CRPSDASATR SRPLATGPSS QSHQEKTTDS GLTEGIWQLV PPSLFKGSHI SQGNEAEERE EPWDHTEKTE EEPVSGSSGS WDQSSQPVFE NVNVKSFDRC TGHSAEHTQC GKPQESTGRG SAFLKAVQGS GDTSRHCLPT LADAKGLQDT GGTVNYFWGI PFCPDGVDPN QYTKVILCQL EVYQKSLKMA QRQLLNKKGF GEPVLPRPPS LIQNECGQGE QASEKNECIS EDMGDEDKEE RQESRASDWH SKTKDFQESS IKSLKEKLLL EEEPTTSHGQ SSQGIVEETS EEGNSVPASQ SVAALTSKRS LVLMPESSAE EITVCPETQL SSSETFDLER EVSPGSRDIL DGVRIIMADK EVGNKEDAEK EVAISTFSSS NQVSCPLCDQ CFPPTKIERH AMYCNGLMEE DTVLTRRQKE AKTKSDSGTA AQTSLDIDKN EKCYLCKSLV PFREYQCHVD SCLQLAKADQ GDGPEGSGRA CSTVEGKWQQ RLKNPKEKGH SEGRLLSFLE QSEHKTSDAD IKSSETGAFR VPSPGMEEAG CSREMQSSFT RRDLNESPVK SFVSISEATD CLVDFKKQVT VQPGSRTRTK AGRGRRRKF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
  • 基因功能參考文獻：
    1. RAP80-BRCA1 complex foci formation is regulated by USP13.USP13 interacts with and deubiquitinates RAP80.RAP80 role in the DNA damage response. PMID: 28569838
    2. The RAP80 deficiency reduces the protein level of p32 and p32 dependent mitochondrial translating proteins such as Rieske and COX1. PMID: 28842250
    3. Low RAP80 mRNA expression correlates with sporadic high-grade serous ovarian carcinoma. PMID: 27443420
    4. RAP80 is a critical gatekeeper in impeding epithelial-mesenchymal transition-induced metastasis and malignant phenotypes of cancer as well as preserving DNA integrity. PMID: 26748910
    5. Data suggest that RAP80 SIM (SUMO interacting motif) binds SUMO-2; both specificity and affinity are enhanced through phosphorylation of canonical CK2 (casein kinase 2) site within the SIM. PMID: 26719330
    6. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. PMID: 26446986
    7. TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.( PMID: 26781088
    8. A new role of FANCG in Homologous recombination repair of interstrand crosslinks through K63Ub-mediated interaction with the Rap80-BRCA1 complex. PMID: 25132264
    9. patients with low RAP80 expression received gemcitabine/cisplatin, those with intermediate/high RAP80 expression and low/intermediate BRCA1 expression received docetaxel/cisplatin PMID: 25164908
    10. Data indicate that a single point deletion (DeltaE81) in RAP80 abrogates multivalent interactions with polyubiquitin. PMID: 24627472
    11. connect ubiquitin- and SUMO-dependent DSB recognition, revealing that RNF4-synthesized hybrid SUMO-ubiquitin chains are recognized by RAP80 to promote BRCA1 recruitment and DNA repair. PMID: 23211528
    12. post-translational phosphorylation of RAP80 by the Cdk1-cyclin B(1) complex is important for RAP80 functional sensitivity to IR and G(2)/M checkpoint control. PMID: 23264621
    13. Loss of RAP80 abolishes the recruitment of the BRCA1-A complex to DNA lesions in response to DNA damage. PMID: 22792303
    14. APC/C(Cdc20) or APC/C(Cdh1) complexes regulate RAP80 stability during mitosis to the G(1) phase, and these events are critical for a novel function of RAP80 in mitotic progression. PMID: 22426463
    15. We show that RNF168, its paralog RNF169, RAD18, and the BRCA1-interacting RAP80 protein accumulate at DNA double strand break sites through the use of bipartite modules composed of ubiquitin binding domains. PMID: 22742833
    16. a model in which SUMO and Ub modification is coordinated to recruit Rap80 and BRCA1 to DNA damage sites. PMID: 22689573
    17. MDC1 is required for the recruitment of RAP80 to DNA double-strand breaks. PMID: 21857162
    18. The interaction between MDC1 and RAP80 requires the tandem BRCT domain of MDC1 and the ubiquitin-interacting motifs of RAP80 PMID: 21622030
    19. a model in which the BRCA1-RAP80 complex limits nuclease accessibility to DSBs, thus preventing excessive end resection and potentially deleterious homology-directed DSB repair mechanisms that can impair genome integrity. PMID: 21335604
    20. RAP80/BRCA1 complexes suppress excessive double-strand break end processing, HR-type double-strand break repair, and overt chromosomal instability PMID: 21406551
    21. a novel nuclear protein that interacts with the retinoid-related testis-associated receptor PMID: 12080054
    22. Abraxas and RAP80 were required for DNA damage resistance, G(2)-M checkpoint control, and DNA repair. PMID: 17525340
    23. data support a model wherein ubiquitin chains at DNA damage sites are used as a targeting mechanism by specific BRCA1 complexes; RAP80 may represent a new class of DNA repair proteins that uses tandem UIM domains as part of its recruitment to DSBs PMID: 17525341
    24. identification of receptor-associated protein 80 (RAP80) as a BRCA1-interacting protein in humans PMID: 17525342
    25. RAP80/UIMC1, the protein highly expressed in testis, was identified as a new cancer-associated antigen. PMID: 17562356
    26. the ubiquitin-interacting motif containing protein RAP80 interacts with BRCA1 and functions in DNA damage repair response PMID: 17621610
    27. RAP80 interacts with the SUMO-conjugating enzyme UBC9 and is a novel target for sumoylation PMID: 17698038
    28. the human Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage PMID: 18077395
    29. Mutational analysis in 168 multiple-case breast/ovarian cancer families, negative for mutations in BRCA1 or BRCA2, suggests that RAP80 does not play an important role as a high penetrance breast cancer susceptibility gene. PMID: 18270812
    30. Truncating mutations of the RAP80 gene do not seem to be a cause of familial breast cancer. A novel RAP80 haplotype or rare missense mutations (p.Ala342Thr, p.Met353Thr and p.Tyr575Asp) may be associated with a modest increased risk of breast cancer. PMID: 18306035
    31. UV irradiation induces translocation of RAP80 to DNA damage foci that colocalize with gamma-H2AX PMID: 18519686
    32. Depletion of RAP80 or RNF8 impairs the translocation of BRCA1 to DNA damage sites and results in defective cell cycle checkpoint control and DSB repair PMID: 18550271
    33. it seems unlikely that moderate to highly penetrant alleles of either RAP80 or Abraxas, confer a significantly high relative risk of breast cancer. PMID: 18695986
    34. MERIT40 represents a novel factor that links BRCA1-Rap80 complex integrity, DSB recognition, and ubiquitin chain hydrolytic activities to the DNA damage response. PMID: 19261746
    35. critical constitutional mutations in RAP80 abrogate DNA damage responses (DDR) function and may be involved in genetic predisposition to cancer. PMID: 19305427
    36. findings show how the sequence between the Rap80 ubiquitin interacting motifs positions the domains for efficient avid polyubiquitin binding across a single K63 linkage, thus defining selectivity PMID: 19328070
    37. RAP80 was a significant factor for survival in patients treated according to BRCA1 levels PMID: 19415121
    38. Data show that it can function in an autoregulatory loop consisting of RAP80, HDM2, and the p53 master regulatory network, implpying an important role for this loop in genome stability and oncogenesis. PMID: 19433585
    39. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19448621
    40. Observational study and meta-analysis of gene-disease association. (HuGE Navigator) PMID: 19064572
    41. Observational study of gene-disease association. (HuGE Navigator) PMID: 18270812

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  • 亞細胞定位：
    Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs).
  • 蛋白家族：
    RAP80 family
  • 組織特異性：
    Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 30298

    OMIM: 609433

    KEGG: hsa:51720

    STRING: 9606.ENSP00000366434

    UniGene: Hs.232721