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  4. Recombinant Human Cell division control protein 6 homolog (CDC6)

Recombinant Human Cell division control protein 6 homolog (CDC6)

  • 中文名稱：
    Recombinant Human Cell division control protein 6 homolog(CDC6),Yeast
  • 貨號：
    CSB-YP857873HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human Cell division control protein 6 homolog(CDC6),Yeast
  • 貨號：
    CSB-EP857873HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Human Cell division control protein 6 homolog(CDC6),Yeast
  • 貨號：
    CSB-EP857873HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Human Cell division control protein 6 homolog(CDC6),Yeast
  • 貨號：
    CSB-BP857873HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Human Cell division control protein 6 homolog(CDC6),Yeast
  • 貨號：
    CSB-MP857873HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Cdc 18L; Cdc 6; CDC18 (cell division cycle 18; S.pombe; homolog) like; CDC18 (S.pombe); Cdc18 related protein; CDC18(S.pombe); Cdc18-related protein; Cdc18L; cdc6; CDC6 cell division cycle 6 homolog; CDC6 related protein; CDC6-related protein; CDC6_HUMAN; Cdc6p; CELL CYCLE CONTROLLER CDC6; Cell division control protein 6; Cell division control protein 6 homolog; Cell division cycle 6 homolog (S. cerevisiae); Cell division cycle 6 homolog; Cell division cycle 6; S. cerevisiae; homolog of; HsCDC 6; HsCDC18; HsCDC6; p62; p62(cdc 6); p62(cdc6)
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-560
  • 氨基酸序列
    MPQTRSQAQA TISFPKRKLS RALNKAKNSS DAKLEPTNVQ TVTCSPRVKA LPLSPRKRLG DDNLCNTPHL PPCSPPKQGK KENGPPHSHT LKGRRLVFDN QLTIKSPSKR ELAKVHQNKI LSSVRKSQEI TTNSEQRCPL KKESACVRLF KQEGTCYQQA KLVLNTAVPD RLPAREREMD VIRNFLREHI CGKKAGSLYL SGAPGTGKTA CLSRILQDLK KELKGFKTIM LNCMSLRTAQ AVFPAIAQEI CQEEVSRPAG KDMMRKLEKH MTAEKGPMIV LVLDEMDQLD SKGQDVLYTL FEWPWLSNSH LVLIGIANTL DLTDRILPRL QAREKCKPQL LNFPPYTRNQ IVTILQDRLN QVSRDQVLDN AAVQFCARKV SAVSGDVRKA LDVCRRAIEI VESDVKSQTI LKPLSECKSP SEPLIPKRVG LIHISQVISE VDGNRMTLSQ EGAQDSFPLQ QKILVCSLML LIRQLKIKEV TLGKLYEAYS KVCRKQQVAA VDQSECLSLS GLLEARGILG LKRNKETRLT KVFFKIEEKE IEHALKDKAL IGNILATGLP
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
  • 基因功能參考文獻：
    1. DNA replication licensing factor Cdc6 is recruited to the proximal side of the centrioles via cyclin A to negatively regulate centrosome duplication by binding and inhibiting the cartwheel protein Sas-6 from forming a stable complex with another centriole duplication core protein, STIL. PMID: 28447620
    2. Results show that both Cdc6 and Cdt1, when expressed in a high level, alone or in combination, were significantly associated with poorer survival in the breast cancer patient cohort. In line with this finding, the expression of Cdc6 and Cdt1 was upregulated in breast cancer cells compared to normal breast epithelial cells. Expression of Cdc6 and Cdt1 was significantly higher in ER negative breast cancer. PMID: 28428557
    3. Results suggested that Cdc6 controls centrosome duplication in a manner independent of its recruitment of PCM proteins to the centrosome. PMID: 29237113
    4. WHSC1L1 and H3K36me2 are enriched in the gene bodies of the cell cycle-related genes CDC6 and CDK2, implying that WHSC1L1 directly regulates the transcription of these gene PMID: 27285764
    5. Cdc6 expression is up-regulated in bladder cancer tissues and is positively correlated to high tumor grade. PMID: 27246979
    6. PPP2R3B codes for the PR70 protein, a regulatory substrate-recognizing subunit of protein phosphatase 2A. PR70 decreased melanoma growth by negatively interfering with DNA replication and cell cycle progression through its role in stabilizing CDC6-chromatin licensing and CDT1 interaction PMID: 27974665
    7. Data suggest that regulation of microtubule nucleation and recruitment of pericentriolar proteins to centrosome requires Cdc6 ATPase activity, as well as centrosomal localization of Cdc6. PMID: 28827311
    8. this study provides a novel regulatory signaling pathway of CDC6-regulated epithelial ovarian cancer growth PMID: 26920249
    9. The data demonstrate that Cdc6 serves as a regulator of ribosomal DNA transcription initiation, and indicate a mechanism by which initiation of ribosomal DNA transcription and DNA replication can be coordinated in cells. PMID: 26872786
    10. Cdc6 interacts with centrosomes during mitotic cell division. PMID: 25875233
    11. The current study suggests that miR26a, miR26b, and CDC6 and factors regulating their expression represent potential cancer diagnostic and prognostic markers as well as anticancer targets. PMID: 25100863
    12. The cell cycle-dependent centrosomal localization of Cdc6 in S and G2 phases suggest a novel function of Cdc6 in centrosomes. PMID: 25498505
    13. Cdk1 contributes to the nuclear export of Cdc6 at the S-to-G2 transition. PMID: 24434580
    14. The Cdc6 is the AAA+ ATPase that assembles prereplicative complexes on replication origins in eukaryotic chromosomes and two more functions in mammalian cells to promote cell proliferation and survival. PMID: 22908236
    15. These results provided new evidence for the regulatory mechanism of aberrant CDC6 oncogene transcription by FOXM1 and AR, two highly expressed transcription factors in prostate cancer cells PMID: 24583551
    16. Two nuclear export signals of Cdc6 work cooperatively and distinctly for the cytoplasmic translocation of Cdc6 phosphorylated by cyclin A/Cdk2. PMID: 24216307
    17. ORC/Cdc6/MCM2-7 complex is a new regulatory mechanism for the helicase. PMID: 23803736
    18. the Nedd8 E1 inhibitor MLN4924 increased CDC6 protein expression due to a delay in cell cycle progression from G1 to S phase, resulting in accumulation of cells with high CDC6 protein levels. PMID: 23597704
    19. Downregulation of Cdc6 effectively inhibits the proliferation of Tca8113 cells. PMID: 22974333
    20. Norcantharidin can induce the degradation of Cdc6 in cancer cells to produce an anti-cancer effect. PMID: 20813683
    21. the up-regulation of CDK2 by CUL4B is achieved via the repression of miR-372 and miR-373, which target CDK2. PMID: 23479742
    22. The phosphorylation of S74 in Cdc6 could be important for binding to the nuclear export protein for cytoplasmic translocalization. PMID: 23129444
    23. CDC6 and GDF-9 might be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma. PMID: 23011157
    24. Ectopic GATA1 stimulates endogenous CDC6 expression in hematopoietic and non-hematopoietic cells. PMID: 22871742
    25. Data show that purified Epstein-Barr nuclear antigen 1 EBNA1 recruits purified Human Orc1 and Cdc6 onto replication origin oriP. PMID: 22589552
    26. novel role for CDC6 in abetting the oncogenic sabotage carried out by HBx and support the paradigm that pre-replicative complex proteins have a role in oncogenic transformation. PMID: 22523071
    27. Cdc6 protein was distributed around the spindle precursor at the time of germinal vesicle breakdown (GVBD), and localized to the margin of the nascent spindle early in prometaphase. PMID: 22262174
    28. Cdc6 the AAA+ ATPase activated p27-bound Cdk2 in its ATPase and cyclin binding motif-dependent manner but only after the p27 bound to the Cdk2 was phosphorylated at the C terminus. PMID: 22223646
    29. Phosphorylation of Cdc6 by polo-like kinase 1 (Plk1), one of the essential mitotic kinases, regulates mitotic exit. PMID: 21519187
    30. The Cdc6 acts as a molecular switch at the E-cadherin locus, linking transcriptional repression to activation of replication. PMID: 22201124
    31. Cdc6 expression is required for CDK2 chromatin association and kinase activation in stimulated primary human fibroblasts. PMID: 21088490
    32. The CDC6 G1321A polymorphism may contribute to the risk of cervical cancer. PMID: 20606534
    33. The expression of CDC6 was positively correlated with hMSH2 in cervical cancer. PMID: 19950599
    34. Nuclear organization of DNA replication initiation proteins PMID: 11779870
    35. CHROMATIN CELLL CYCLE PMID: 11846807
    36. downregulation in prostate cancer PMID: 12006585
    37. HuCdc6 is cleaved by caspase 3 during apoptosis to prevent a wounded cell from replicating and to facilitate death PMID: 12151338
    38. Results show that geminin, cdt1 and cdc6 are differentially regulated during megakaryocytic differentiation and suggest an active role of cdc6 in endomitosis. PMID: 12429841
    39. Data show that human Cdc6 (HuCdc6) regulates the onset of mitosis, as overexpression of HuCdc6 in G(2) phase cells prevents entry into mitosis. PMID: 12554670
    40. caspase-mediated cleavage of Cdc6 creates a truncated Cdc6 fragment that is retained in the nucleus and induces apoptosis PMID: 14517333
    41. endogenous Cdc6 remains nuclear and chromatin bound throughout the entire S period PMID: 14749377
    42. Cdc6 expression is regulated during megakaryocytic differentiation through transcriptional mechanisms involving a novel E2 box-GATA element. PMID: 15590906
    43. Results further confirm the importance of CDC6 in malignant transformation and in the pathogenesis of cervical dysplasia. PMID: 15696126
    44. Androgen receptor may play important role in onset of DNA synthesis in prostate cancer cells by regulating expression and stability of Cdc6, critically required for assembly of pre-replication complex. PMID: 15887248
    45. initiation of DNA replication is regulated by p53 through Cdc6 protein stability PMID: 16055707
    46. Cdc6 is not only required for G1 origin licensing, but is also crucial for proper S-phase DNA replication that is essential for DNA segregation during mitosis PMID: 16439999
    47. Truncated Cdc6 proteins act as dominant-negative inhibitors of replication initiation and disrupt chromatin structure and/or induce DNA damage, leading to ATM/ATR kinase activation and p53-Bax-mediated apoptosis PMID: 16801388
    48. These findings demonstrate an important and conserved role for Huwe1 in regulating Cdc6 abundance after DNA damage. PMID: 17567951
    49. Cdc6 plays a key role in the sequential molecular events leading to repression of origin licensing and loss of proliferative capacity during execution of the differentiation programme. PMID: 17689530
    50. hCdt1 and hCdc6 expression promote malignant behavior PMID: 18006835

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  • 相關疾?。?/div>
    Meier-Gorlin syndrome 5 (MGORS5)
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    CDC6/cdc18 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1744

    OMIM: 602627

    KEGG: hsa:990

    STRING: 9606.ENSP00000209728

    UniGene: Hs.405958