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  4. Recombinant Human Cytosolic phospholipase A2 (PLA2G4A)

Recombinant Human Cytosolic phospholipase A2 (PLA2G4A)

  • 中文名稱：
    人PLA2G4A重組蛋白
  • 貨號：
    CSB-YP018097HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人PLA2G4A重組蛋白
  • 貨號：
    CSB-EP018097HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人PLA2G4A重組蛋白
  • 貨號：
    CSB-EP018097HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人PLA2G4A重組蛋白
  • 貨號：
    CSB-BP018097HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人PLA2G4A重組蛋白
  • 貨號：
    CSB-MP018097HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Calcium dependent phospholipid binding protein; CPLA 2; cPLA2 alpha; cPLA2; Cytosolic phospholipase A2; Cytosolic phospholipase A2 group IVA; Lysophospholipase; MGC126350; PA24A_HUMAN; Phosphatidylcholine 2 acylhydrolase; Phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group 4 A; Phospholipase A2 group IVA (cytosolic calcium dependent); Phospholipase A2 group IVA; PhospholipaseA2; PLA2G4; pla2g4a
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-749
  • 氨基酸序列
    MSFIDPYQHI IVEHQYSHKF TVVVLRATKV TKGAFGDMLD TPDPYVELFI STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE TLGTATFTVS SMKVGEKKEV PFIFNQVTEM VLEMSLEVCS CPDLRFSMAL CDQEKTFRQQ RKEHIRESMK KLLGPKNSEG LHSARDVPVV AILGSGGGFR AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH PDFPEKGPEE INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG ETLIHNRMNT TLSSLKEKVN TAQCPLPLFT CLHVKPDVSE LMFADWVEFS PYEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL FNRVLGVSGS QSRGSTMEEE LENITTKHIV SNDSSDSDDE SHEPKGTENE DAGSDYQSDN QASWIHRMIM ALVSDSALFN TREGRAGKVH NFMLGLNLNT SYPLSPLSDF ATQDSFDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN KLPFPKIDPY VFDREGLKEC YVFKPKNPDM EKDCPTIIHF VLANINFRKY RAPGVPRETE EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMHFN TLNNIDVIKE AMVESIEYRR QNPSRCSVSL SNVEARRFFN KEFLSKPKA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production. Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway. In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific. Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides. Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis.
  • 基因功能參考文獻(xiàn)：
    1. PLA2G4A up-regulated the protein expression of E-cadherin and down-regulated the protein expression of vimentin, which inhibited ESCC cell mobility and invasiveness. PMID: 30071514
    2. The impaired phospholipid level and remarkable increased in cPLA2 concentration asserted their roles in the etiology of autism. PMID: 28724385
    3. cPLA2alpha, which releases fatty acid from the sn-2 position of membrane-associated glycerophospholipids, is critically involved in coronavirus replication, most likely by producing lysophospholipids that are required to form the specialized membrane compartments in which viral RNA synthesis takes place PMID: 29167338
    4. results suggested that IFN-gamma induces autophagy-associated apoptosis in CRC cells via inducing cPLA2-dependent mitochondrial ROS production. PMID: 29551681
    5. These findings indicate the positive roles of cPLA2alpha in breast cancer cell growth, survival, migration and response to chemotherapy. Our work also highlights the therapeutic value of blocking cPLA2alpha to overcome chemoresistance in breast cancer. PMID: 29307829
    6. These results indicate epigenetic regulation of cPLA2 and the potential of such regulation for treatment of chronic inflammation. PMID: 26162318
    7. cPLA2alpha may play a key role in renal repair after injury through a PGE2-independent mechanism PMID: 27554058
    8. This study indicates the potential role of cPLA2alpha in breast cancer metastasis and indicates that this molecule is a promising therapeutic target for breast cancer. PMID: 28383549
    9. these studies suggests that cPLA2alpha expressed by neutrophils, but not epithelial cells, plays a significant role in infection-induced neutrophil transepithelial migration by mediating LTB4 synthesis during migration, which serves to amplify the magnitude of neutrophil recruitment in response to epithelial infection PMID: 28887431
    10. cPLA2alpha activates PI3K/AKT and inhibits Smad2/3 during epithelial-mesenchymal transition of hepatocellular carcinoma cells. PMID: 28649002
    11. Phospholipase activity is linked to energy metabolism, revealing cPLA2 as a central regulator of both lipidomics and energy flux. PMID: 27133131
    12. present study indicates that among all cytosolic phospholipases A2, specific phospholipase A2 group IVA are the main enzymes involved in cigarette smoke induced anomalies in type I and type II lung epithelial cells PMID: 27528014
    13. genetic association study in Quebec City population: Data suggest total plasma n-6 fatty acid phospholipid levels and C-reactive protein are modulated by SNPs in PLA2G4A and PLA2G6 alone or in combination with fish oil dietary supplementation. PMID: 26525102
    14. cPLA2alpha plays an important role in cell cycle re-entry by quiescent prostate cancer cells PMID: 26416244
    15. Letter: report instability of cytosolic phospholipase A2alpha variant resulting in severe impairment in platelet arachidonic acid metabolism. PMID: 25904158
    16. Thymocyte maturation can occur independently of cPLA2-alpha. PMID: 25969996
    17. These studies constitute a definitive account, demonstrating the fundamental role of cPLA2alpha to eicosanoid formation and cellular responses within the human circulation. PMID: 26183771
    18. The study reports the formation of a multiprotein complex at the G2-to-M transition in vitro and in vivo. Group IVA cytosolic phospholipase A2 (cPLA2-alpha) acts as a bridge in this complex to promote binding of SIRT2 to cyclin A-Cdk2. PMID: 26303530
    19. Case Report/Twin Study: syndromic form of deficiency of cPLA2a , characterised by recurrent GI ulcers and bleeding diathesis, associated with mild inherited deficiency of factor XI. PMID: 25102815
    20. EGF stimulates platelet-activating factor production in ovarian cancer cells in a manner that requires cPLA2. PMID: 24721622
    21. We conclude that cPLA2alpha is required for sustaining AKT phosphorylation at Ser473 and cell proliferation in colorectal cancer cells with PI3K mutation PMID: 25365190
    22. alphaSN triggered synapse damage via hyperactivation of cPLA2 PMID: 25761116
    23. Suggest retinal pigment epithelium metabolism of 7-ketocholesterol occurs by esterification to fatty acids via cPLA2alpha and SOAT1 followed by selective efflux to HDL. PMID: 25617738
    24. Data indicate 3-(1-Aryl-1H-indol-5-yl)propanoic acids a potent inhibitor of cytosolic phospholipase A2alpha (cPLA2alpha). PMID: 25102418
    25. cPLA2alpha appears to be an important regulator of central effectors of inflammation and joint destruction, namely MMP3, IL8, COX2, and PGE2. PMID: 24349530
    26. Phospholipase A2 expression in coronary thrombus is associated with recurrence of cardiac events. PMID: 23948114
    27. LMW HA was a potent stimulant of AA release in a time- and dose-dependent manner, induced cPLA2alpha, ERK1/2, p38, and JNK phosphorylation, as well as activated COX2 expression and prostaglandin (PG) E2 production PMID: 24366870
    28. data provide new and important contributions to the understanding of cPLA2alpha regulation at the transcriptional level, with implications for eicosanoid metabolism, cellular signaling, and disease pathogenesis PMID: 23549331
    29. Mutations in PLA2G4A were identified as a cause of cryptogenic multifocal ulcerating stenosing enteritis in two affected siblings. PMID: 23268370
    30. LacCer is a direct activator of cPLA2alpha. PMID: 23801329
    31. Data detected statistically significant but weak impact of PLA2G4A and PTGS2 gene polymorphisms on niacin flush response in schizophrenia patients. PMID: 23219238
    32. PLA2G4A might serve as a promising target for future therapeutic approaches to gastric cancer combined with COX-2 inhibitors. PMID: 23307260
    33. results suggest that C2-di-ethyl-ceramide-1-phosphate appeared to inhibit cPLA(2)alpha, probably by interaction with a site in the catalytic domain of the enzyme. PMID: 23043861
    34. The presence of a terminal sialic acid moiety, a rare modification of mammalian glycosylphosphatidylinositol anchors, was essential in the activation of cytosolic phospholipase A2 and synapse damage induced by cross-linked PrPc. PMID: 22895089
    35. the regulation of HT-29 proliferation is mediated by cPLA(2)alpha-dependent PGE(2) production. PGE(2)via EP induces CREB phosphorylation by the PKA pathway and regulates beta-catenin and cyclin D1 cellular localization by PKB/Akt pathway. PMID: 22728329
    36. study identified the MAPK/ERK regulated, cytosolic, calcium-dependent PLA2G4A as a novel hepatitis C virus dependency factor; inhibition of PLA2G4A activity reduced core protein abundance at lipid droplets, core envelopment and secretion of particles PMID: 22911431
    37. A VE-cadherin-PAR3-alpha-catenin complex regulates the Golgi localization and activity of cytosolic phospholipase A(2)alpha in endothelial cells. PMID: 22398721
    38. The molecular and biological evidence of the involvement of cPLA2s in the pathogenesis of Alzheimer's disease, is reviewed. PMID: 22648535
    39. the lipid modifier cPLA2alpha and EHD1 are involved in the vesiculation of CD59-containing endosomes PMID: 22456504
    40. Immunohistochemical expressions of cPLA(2)alpha in 23 species of human non-small lung cancer and 5 species of human normal lung to assess their clinicopathological relevance. PMID: 22274867
    41. genetic association studies in populations in Costa Rica: An SNP in PLA2G4A (rs12746200) is associated with myocardial infarction; this association is modulated by dietary factors (i.e., omega-6 fatty acid intake). PMID: 22378731
    42. these results illustrated that cPLA(2)alpha-mediated actin filament rearrangements downstream of Akt activation is required for C. sakazakii invasion into brain endothelial cells. PMID: 22138395
    43. findings indicate that cPLA2 in the lens epithelial cells is regulated by calcium mobilization and mitogen-activated protein kinases (MAPK) activation; both PDGF mitogenic action and calcium signaling are cPLA(2)alpha-dependent PMID: 21896865
    44. There is a possible relation between Leptin release and cPLA 2 activity in inflammatory cells induced by LPS. PMID: 21315000
    45. These results provide in vivo evidence for increased expression and activation of cPLA2 in motor neurons, reactive astrocytes, and activated microglia in amyotrophic lateral sclerosis PMID: 20066429
    46. cPLA(2)alpha gene activation by IL-1beta is dependent on an upstream kinase pathway, enzymatic activation and downstream 15-lipoxygenase activity: a positive feedback loop PMID: 21771656
    47. A cPLA2-initiated lipid mediator pathway induces autophagy in a murine macrophage cell line and in human primary monocytes. PMID: 22003202
    48. Mutation of HuR Thr-118 reduced the association between HuR and cPLA(2)alpha mRNA under IL-1beta treatment PMID: 21862584
    49. These findings demonstrate that specific host factors involving cPLAalpha and cysteinyl LTs contribute to type III GBS penetration of the blood-brain barrier and their contribution involves PKCalpha. PMID: 21825068
    50. There were relatively more cPLA2alpha positive tumors, as defined by positive staining in >10% of tumor cells 24 of 76 tumors (32%). PMID: 21198294

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  • 相關(guān)疾病：
    PLA2G4A mutations resulting in phospholipase A2 deficiency have been found in a patient affected by recurrent episodes of multiple complicated ulcers of the small intestine, not due to cyclooxygenase inhibitors use. Disease features also include platelet dysfunction, and globally decreased eicosanoid synthesis (PubMed:18451993).
  • 亞細(xì)胞定位：
    Cytoplasm. Golgi apparatus membrane. Nucleus envelope.
  • 組織特異性：
    Expressed in various cells and tissues such as macrophages, neutrophils, fibroblasts and lung endothelium. Expressed in platelets (at protein level).
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9035

    OMIM: 600522

    KEGG: hsa:5321

    STRING: 9606.ENSP00000356436

    UniGene: Hs.497200