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  4. Recombinant Human DNA damage-binding protein 2 (DDB2)

Recombinant Human DNA damage-binding protein 2 (DDB2)

  • 中文名稱：
  • 貨號(hào)：
    CSB-EP846067HU
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    E.coli
  • 分子量：
    53.8 kDa
  • 表達(dá)區(qū)域：
    1-427aa
  • 氨基酸序列
    MAPKKRPETQKTSEIVLRPRNKRSRSPLELEPEAKKLCAKGSGPSRRCDSDCLWVGLAGPQILPPCRSIVRTLHQHKLGRASWPSVQQGLQQSFLHTLDSYRILQKAAPFDRRATSLAWHPTHPSTVAVGSKGGDIMLWNFGIKDKPTFIKGIGAGGSITGLKFNPLNTNQFYASSMEGTTRLQDFKGNILRVFASSDTINIWFCSLDVSASSRMVVTGDNVGNVILLNMDGKELWNLRMHKKKVTHVALNPCCDWFLATASVDQTVKIWDLRQVRGKASFLYSLPHRHPVNAACFSPDGARLLTTDQKSEIRVYSASQWDCPLGLIPHPHRHFQHLTPIKAAWHPRYNLIVVGRYPDPNFKSCTPYELRTIDVFDGNSGKMMCQLYDPESSGISSLNEFNPMGDTLASAMGYHILIWSQEEARTRK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR.; Inhibits UV-damaged DNA repair.; Inhibits UV-damaged DNA repair.
  • 基因功能參考文獻(xiàn)：
    1. Study identified novel mutation DDB2 p. (Lys381Argfs*2) present at a homozygous state in 5 affected cases belonging to the same Tunisian family with Xeroderma pigmentosum. PMID: 29169765
    2. Chronic low-dose of UVB (CLUV) treatment activates p53, which corroborate with the increased level of DDB2 and XPC proteins. DDB2 and XPC recruited at chromatin bound, suggesting a more efficient cyclobutane pyrimidine dimer (CPD) recognition by NER and more efficient repair of CDP. PMID: 29448173
    3. DDB2-PCNA interaction may contribute to a correct DNA damage response for maintaining genome integrity PMID: 29604309
    4. DDB2 is modified by SUMOylation upon ultraviolet irradiation, and this post-translational modification plays an important role in the initial recognition and processing of ultraviolet irradiation-induced DNA damage occurring within the context of chromatin. PMID: 28981631
    5. Results show that DDB2 is critical for chromatin association of XRCC5/6 in the absence of DNA damage and provide evidence that XRCC5/6 are functional partners of DDB2 in its transcriptional stimulatory activity. PMID: 28035050
    6. UVRAG is a regulator of CRL4(DDB2)-mediated nucleotide excision repair and its expression levels may influence melanoma predisposition. PMID: 27203177
    7. High DDB2 expression is associated with increased radioresistance of non-small cell lung cancer. PMID: 27553023
    8. data revealed that DDB2 is involved in early events occurring during metastatic progression of breast cancer cells and will contribute to define this protein as a new marker of metastatic progression in this type of cancer. PMID: 26879405
    9. DDB2 polymorphisms are associated with gastric cancer and atrophic gastritis risks. PMID: 26760766
    10. the release of NER components such as DNA damage binding protein 2 (DDB2) and Xeroderma Pigmentosum complementation group C protein (XPC) following oxidative stress might putatively involve their apoptotic role rather than DNA repair function. PMID: 26263968
    11. PKM2 interacts with DDB2 and reduces cell survival upon UV irradiation. PMID: 26410533
    12. DDB2 can bind to the promoter region of NEDD4L and recruit enhancer of zeste homolog 2 histone methyltransferase to repress NEDD4L transcription by enhancing histone H3 lysine 27 trimethylation at the NEDD4L promoter. PMID: 26130719
    13. data demonstrated that the DDB2 IRES activity was promoted during stress conditions. These results reveal a novel mechanism contributing to DDB2 expression PMID: 26187069
    14. DDB2 is involved in ubiquitination and degradation of PAQR3 in gastric cancer cells. PMID: 26205499
    15. DDB2 rs747650 is involved in androgen metabolism, inflammation processes and scar formation in severe acne. PMID: 24399259
    16. DDB2 plays an instrumental role in DNA damage induced ROS accumulation, ROS induced premature senescence and inhibition of skin tumorigenesis. PMID: 23109835
    17. DDB2 is protected by XPC from ubiquitination and degradation in a stochastic manner; thus XPC allows DDB2 to initiate multiple rounds of repair events, thereby contributing to the persistence of cellular DNA repair capacity PMID: 25628365
    18. DDB2 protein expression is low in ovarian tumor cells. PMID: 24574518
    19. Chromatin retention of DNA damage sensors DDB2 and XPC through loss of p97 segregase causes genotoxicity. PMID: 24770583
    20. DDB2 is a PCNA-binding protein and this association is required for DDB2 proteolytic degradation. PMID: 24200966
    21. study provides new knowledge about the posttranslational regulation of DDB2 and expands the biological functions of protein alpha-N-methylation to DNA repair PMID: 24753253
    22. the data indicate that p53-dependent upregulation of XPC and DDB2 is a key mechanism upon genotoxic stress, whereby melanoma cells acquire resistance towards DNA cross-linking agents. PMID: 23604128
    23. DDB2 and a DDB2-ATM feedback loop influence HCMV replication. PMID: 24335308
    24. Knockdown of DDB2-induced IkappaBalpha gene expression restored NF-kappaB activity. PMID: 23774208
    25. These results suggest that the SUMOylation of DDB2 facilitates CPD repair. PMID: 23860269
    26. DDB2 facilitates ATR and ATM recruitment to the DNA damage site following UV irradiation. PMID: 23422745
    27. Results indicate a transcriptional regulatory pathway of DDB2 that is directly linked to the mechanisms that suppress metastasis of colon cancer. PMID: 23610444
    28. The 3'untranslated region of DDB2 contains a cis-acting element that affects stability, export and translation of the message PMID: 23605047
    29. Data indicate that poly(ADP-ribose) polymerase-1 (PARP-1)collaborates with DNA-binding protein 2 (DDB2) to increase the efficiency of the lesion recognition step of global genomic subpathway of NER (GG-NER). PMID: 23319653
    30. DDB2 can inhibit cell growth rate in AR-expressing cells (LNCaP) but not in AR-null cells (PC3). PMID: 22846800
    31. data support a model in which poly(ADP-ribosyl)ation of DDB2 suppresses DDB2 ubiquitylation and outline a molecular mechanism for PARP1-mediated regulation of nucleotide excision repair through DDB2 stabilization and recruitment of the chromatin remodeler ALC1 PMID: 23045548
    32. study reports a new function of DDB2 in modulating chromatin structure at DNA lesions PMID: 22492724
    33. DDB2 subunit of UV-DDB associates transiently with the DNA-binding domain of XPC to fine-tune its engagement with CPD lesions PMID: 22039351
    34. Nucleotide excision repair proteins rapidly accumulate but fail to persist in human xeroderma pigmentosum XP-E (DDB2 mutant) cells. PMID: 21388382
    35. Multiple skin cancers in adults are associted with mutations in the XP-E (DDB2) DNA repair gene. PMID: 21107348
    36. Overexpression of DDB2 enhances the sensitivity of ovarian cancer cells to cisplatin by augmenting cellular apoptosis. PMID: 20013802
    37. Damaged DNA-binding protein 2 (DDB2) protects against UV irradiation in human cells and Drosophila PMID: 20398405
    38. Data show that XPC and Ku oppositely regulate the ubiquitin ligase activity of DDB2, and that DDB2 complex-mediated ubiquitylation plays a role in recruiting XPA to damaged sites. PMID: 20368362
    39. These results indicate that DDB2 is a modulator of UV-induced apoptosis, and that UV resistance can be overcome by inhibition of DDB2 PMID: 11852074
    40. These results demonstrate direct activation of the human DDB2 gene by p53. The corresponding region in the mouse DDB2 gene shared significant sequence identity with the human gene but was deficient for p53 binding and transcriptional activation. PMID: 11971958
    41. BRCA1 upregulates DDB2, with some evidence that p53 is involved in its regulation. PMID: 12496474
    42. overexpression of DDB2 in V79 cell potentiates DNA repair and protects cells from UV-induced apoptosis and cytotoxicity. PMID: 12553360
    43. DDB2 and CSA are each integrated into nearly identical complexes via interaction with DDB1 PMID: 12732143
    44. DDB2p48 activates the recruitment of XPC to cyclobutane pyrimidine dimers and may be the initial recognition factor in the nucleotide excision repair pathway PMID: 12944386
    45. Data suggest that both before and after UV irradiation, DDB2 directly regulates p53 levels, while DDB2 expression is itself regulated by p53. PMID: 14560002
    46. identification of four DDB2 variants from HeLa cells (D1-D4) that are generated by alternative splicing PMID: 14751237
    47. DDB2 regulates TNF signaling-mediated apoptosis via cFLIP and contributes to acquired cross-resistance. PMID: 15644494
    48. UV-DDB interacts with XPC physically, and both are polyubiquitylated by the UV-DDB-ubiquitin ligase complex. PMID: 15882621
    49. DDB2 has an intrinsic damaged DNA binding activity PMID: 16260596
    50. CUL-4A mediates the proteolytic degradation of DDB2 and this degradation event, initiated at the lesion sites, regulates damage recognition by XPC. PMID: 16527807

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  • 相關(guān)疾?。?/div>
    Xeroderma pigmentosum complementation group E (XP-E)
  • 亞細(xì)胞定位：
    Nucleus. Note=Accumulates at sites of DNA damage following UV irradiation.
  • 蛋白家族：
    WD repeat DDB2/WDR76 family
  • 組織特異性：
    Ubiquitously expressed; with highest levels in corneal endothelium and lowest levels in brain. Isoform D1 is highly expressed in brain and heart. Isoform D2, isoform D3 and isoform D4 are weakly expressed.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2718

    OMIM: 278740

    KEGG: hsa:1643

    STRING: 9606.ENSP00000256996

    UniGene: Hs.700338