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Recombinant Human Developmental pluripotency-associated protein 2 (DPPA2)

  • 中文名稱：
    人DPPA2重組蛋白
  • 貨號(hào)：
    CSB-YP768782HU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    人DPPA2重組蛋白
  • 貨號(hào)：
    CSB-EP768782HU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    人DPPA2重組蛋白
  • 貨號(hào)：
    CSB-EP768782HU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人DPPA2重組蛋白
  • 貨號(hào)：
    CSB-BP768782HU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人DPPA2重組蛋白
  • 貨號(hào)：
    CSB-MP768782HU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DPPA2
  • Uniprot No.：
  • 別名：
    DPPA2; PESCRG1Developmental pluripotency-associated protein 2; Pluripotent embryonic stem cell-related gene 1 protein
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-298
  • 氨基酸序列
    MSDANLDSSK KNFLEGEVDD EESVILTLVP VKDDANMEQM EPSVSSTSDV KLEKPKKYNP GHLLQTNEQF TAPQKARCKI PALPLPTILP PINKVCRDTL RDWCQQLGLS TNGKKIEVYL RLHRHAYPEQ RQDMPEMSQE TRLQRCSRKR KAVTKRARLQ RSYEMNERAE ETNTVEVITS APGAMLASWA RIAARAVQPK ALNSCSIPVS VEAFLMQASG VRWCVVHGRL LSADTKGWVR LQFHAGQAWV PTTHRRMISL FLLPACIFPS PGIEDNMLCP DCAKRNKKMM KRLMTVEK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

問(wèn)答及客戶評(píng)論

■ 常見(jiàn)問(wèn)題解答
Q:

We are interested in the following proteins expressed in mammalian with a concentration of 50ug/ml. Please let me know if this is possible for the following products:
We would also like to know if it’s possible to buy some of the antigen resuspension buffer you use?
We will be using these proteins to build a protein array for serum profiling and are unsure if they need aseptic processing or endotoxin removal?
Can you please advise whether this is necessary? We would like these proteins in eukaryotic host instead with a concentration of 50ug/ml.
CSB-MP009188HU
CSB-MP768782HU
CSB-MP013325HU
CSB-MP887003HU
CSB-MP013348HU
CSB-MP636766XBF
CSB-MP897569HU
CSB-MP009178HU
CSB-MP664879BO

A:
1.Thanks for your inquiry.
Recombinant Human Developmental pluripotency-associated protein 2(DPPA2)
CSB-YP768782HU >> Yeast
CSB-EP768782HU >> E.coli
CSB-BP768782HU >> Baculovirus
CSB-MP768782HU >> Mammalian cell
Expression Region: 1-298aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MSDANLDSSKKNFLEGEVDDEESVILTLVPVKDDANMEQMEPSVSSTSDVKLEKPKKYNPGHLLQTNEQFTAPQKARCKIPALPLPTILPPINKVCRDTLRDWCQQLGLSTNGKKIEVYLRLHRHAYPEQRQDMPEMSQETRLQRCSRKRKAVTKRARLQRSYEMNERAEETNTVEVITSAPGAMLASWARIAARAVQPKALNSCSIPVSVEAFLMQASGVRWCVVHGRLLSADTKGWVRLQFHAGQAWVPTTHRRMISLFLLPACIFPSPGIEDNMLCPDCAKRNKKMMKRLMTVEK


2. As you want these proteins expressed in mammalian with a concentration of 50ug/ml, generally the concentration for MP protein is 0.1-0.5mg/ml (100ug/ml-500ug/ml),
The concentration of 50ug/ml is a little low, the low concentration is not very good for the protein stability, so we recommend the concentration is better higher than 0.1mg/ml.
3. You would also like to know if it’s possible to buy some of the antigen resuspension buffer you use?
How much do you need for the buffer ? If it's not too much, we can provide some for free, such as 5ml or 10ml.
4.You will be using these proteins to build a protein array for serum profiling and are unsure if they need aseptic processing or endotoxin removal?
Can you please advise whether this is necessary? You would like these proteins in eukaryotic host instead with a concentration of 50ug/ml.
According to your application, they don't need aseptic processing or endotoxin removal.
Do you mean that the concentration of 50ug/ml is not necessary, as long as these proteins are expressed in eukaryotic host ?
If yes, we recommend the concentration is better higher than 100ug/ml, and you can choose any eukaryotic expression systems. (YP, BP, MP)

靶點(diǎn)詳情

  • 功能：
    Binds to target gene promoters, including NKX2-5 and SYCE1, but not GATA4, and may be involved in the maintenance of the active epigenetic status of these genes.
  • 基因功能參考文獻(xiàn)：
    1. High DPPA2 Protein Expression is Associated with Gastric Cancer Metastasis PMID: 26745102
    2. results showed that DPPA2 expression was significantly correlated to the depth of tumor invasion and progression of tumor cells through advanced stages in colorectal cancer. PMID: 25497006
    3. A important role of HIWI and DPPA2 in tumorigenesis and also in lymph node metastasis of tumor cells. PMID: 24532429
    4. Differential expression of the embryo/cancer gene ECSA(DPPA2) in human preimplantation development. PMID: 18467432
    5. ECSA/DPPA2 is a promising target for antigen-specific immunotherapy in non-small cell lung cancers PMID: 18519755
    6. In normal tissues, PLAC1 expression was restricted to the placenta while DPPA2 expression was restricted to the placenta and testis. PMID: 19705800

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  • 亞細(xì)胞定位：
    Nucleus.
  • 組織特異性：
    Expressed in embryonic stem cells. No expression is seen in 5 months embryo, mesenchymal stem cells, embryonic fibrocytes and adult tissues.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 19197

    OMIM: 614445

    KEGG: hsa:151871

    STRING: 9606.ENSP00000417710

    UniGene: Hs.351113