Recombinant Human Dickkopf-related protein 1 (DKK1), partial Escherichia Coli
In Stock-
中文名稱(chēng):人DKK1重組蛋白
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貨號(hào):CSB-EP006920HU
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規(guī)格:¥1344
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圖片:
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:
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Uniprot No.:
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別名:Dickkopf 1; Dickkopf 1 homolog; Dickkopf 1 like; Dickkopf homolog 1; Dickkopf like protein 1; Dickkopf related protein 1; Dickkopf WNT signaling pathway inhibitor 1; Dickkopf-1; Dickkopf-related protein 1; DKK 1; Dkk-1; Dkk1; DKK1_HUMAN; hDkk 1; hDkk-1; SK
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種屬:Homo sapiens (Human)
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蛋白長(zhǎng)度:partial
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來(lái)源:E.coli
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表達(dá)區(qū)域:33-266
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氨基酸序列LNSVLNSN AIKNLPPPLG GAAGHPGSAV SAAPGILYPG GNKYQTIDNY QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH TKGQEGSVCL RSSDCASGLC CARHFWSKIC KPVLKEGQVC TKHRRKGSHG LEIFQRCYCG EGLSCRIQKD HHQASNSSRL HTCQRH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. -
蛋白標(biāo)簽:N-terminal 6xHis-tagged
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產(chǎn)品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. -
儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:3-7 business days
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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產(chǎn)品描述:
The preparation of Recombinant Human DKK1 protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This DKK1 was expressed in E.coli. According to SDS-PAGE, the purity turns out to be 85%+.
DKK1, a secreted protein, was first found in Xenopus and involved in the head formation and limb morphogenesis during vertebrate development. Abnormal expression of DKK1 has now emerged as an important regulator in a variety of human cancers. For example, some reports discovered that DKK1 was overexpressed in hepatocellular carcinoma (HCC) and myeloma, acting as a tumor promoter. By contrast, DKK1 expression was downregulated in renal cell carcinoma and colorectal cancers, indicating that it might function as a tumor suppressor. Certain reports suggested that the downregulation of DKK1 increased proliferation of epithelial cells in the large intestine, which was associated with increased transcriptional activity of β-catenin. While some findings implied that DKK1 overexpression inhibits proliferation and migration in human retinal pigment epithelial cells via the Wnt/β-catenin signaling pathway.
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Datasheet & COA:Please contact us to get it.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity.
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基因功能參考文獻(xiàn):
- Results provide evidence that DKK-1 is involved in the progression of prostate cancer (PC). DKK-1-mediated inhibition of osteoblasts, which contributes to tumor progression and osteolytic metastases, may also play a role in the development of metastases with osteoblastic features. PMID: 30116403
- our findings suggest that glucocorticoid-induced expression of Dkk-1 could be a key factor in modulating the differentiation ability of MSCs used for osteonecrosis of the femur head and other stem cell therapies. PMID: 29084466
- MicroRNA152 inhibits cell proliferation of osteosarcoma through DKK1. PMID: 29845282
- this study shows that DKK1 is increased in vitiligo in lesional and peri-lesional skin of acral and non-acral lesions PMID: 29605863
- Dkk-1 and PTH levels in psoriatic arthritis are lower than healthy female controls, in contrast with rheumatoid arthritis, in which they are increased PMID: 28634697
- this study suggests that higher expression of DKK1 in the dermis induced senescence in melanocytes that may lead to hypopigmentation and play role in vitiligo pathogenesis PMID: 29442138
- TNF-alpha, DKK1, and OPG have roles in pathogenesis of knee osteoarthritis PMID: 28676900
- Serum level of DKK-1 is elevated in patients with rheumatoid arthritis (RA) compared to healthy controls, suggesting an important role of DKK-1 in the pathogenesis and treatment of RA [review]. PMID: 29665496
- the staining intensity of DKK1 was significantly stronger in the placentas of the severe preeclampsia group PMID: 29603045
- Serum OPN and DKK1 levels of hepatocellular carcinoma patients could be considered as novel biomarkers showing prognostic significance after hepatectomy based on long-term survival data PMID: 29753515
- These findings suggested that decreased circulating DKK-1 levels are associated with the development and severity of exudative age-related macular degeneration, and have potential to become a biomarker for exudative age-related macular degeneration. PMID: 28455497
- In patients with rheumatoid arthritis, higher levels of DKK1 were found in patients with lower functional status.This could be explained by more structural damage; DKK1 could be used as a biomarker of joint destruction in RA. PMID: 29282619
- The Dickkopf-1 (DKK1) is a secreted inhibitor of beta-catenin-dependent Wnt signalling and was originally characterized as a tumour suppressor based on the prevailing view that Wnt signalling promotes cancer pathogenesis. PMID: 28574171
- Collectively, our data strongly suggest that stress-related neurohormones, such as GCs, cause DP cells to secrete DKK1, which in turn inhibits hair growth. PMID: 28155238
- the inhibitory effects of silencing miR-217 on stem cell properties and Wnt signaling were reversed by the downregulation of DKK1 in miR-217-downregulated cells. Therefore, our results indicate that miR-217 plays a vital role in the CSC-like phenotypes of Hepatocellular carcinoma (HCC) cells and may be used as a potential therapeutic target against HCC. PMID: 28849121
- In our cohort of pregnant women, sclerostin and DKK1 were not associated with any adverse metabolic profile, and possibly do not play relevant roles in the pathophysiology of gestational diabetes mellitus. PMID: 28277306
- Serum DKK-1 levels in axial spondyloarthritis depend on disease duration. As disease duration increases, DKK-1 serum levels decrease. PMID: 27297260
- inhibition of the mTOR pathway increases sensitivity of melanoma cells towards temozolomide, which is associated with enhanced expression of the Wnt antagonist DKK1 PMID: 28423208
- Inhibition of DKK1 expression effectively decrease plaque stability by attenuating endoplasmic reticulum stress-mediated cellular apoptosis through intiating the JNK pathway and inhibiting Wnt/betacatenin and following by activation of the IRE1alpha and eif2alpha/CHOP pathways. PMID: 28703797
- This study demonstrated that DKK-1 may be a predictor for prognosis in pancreatic ductal adenocarcinoma patients. PMID: 28749252
- synovial fluid Dkk-1 levels are positively related to the severity of joint damage in knee osteoarthritis. PMID: 28451870
- DKK1 exerts its pro-invasion function at least in part through the beta-catenin/ MMP-7 signaling pathway, suggesting DKK1 as a potential therapeutic target for human hilar cholangiocarcinoma (HCCA). PMID: 27608843
- Sclerostin But Not Dickkopf-1 has roles in increasing prevalence of osteoporotic fracture and lower bone mineral density in postmenopausal Korean women PMID: 27289555
- High serum levels of sclerostin and Dkk-1 are associated with acute ischaemic stroke PMID: 27573735
- regulated by Dkk-1 secreted by Multiple Myeloma cells and is a potential mechanism contributing to the osteoblast dysfunction noted in Multiple Myeloma. PMID: 26763740
- DKK1 c.121G>A (p.(A41T)) variant was identified in the family with Chiari malformation type 1. Screening of DKK1 in a cohort of 65 CMI sporadic patients identified another missense variant, the c.359G>T (p.(R120L)), in two unrelated patients. PMID: 28513615
- LRP6 ectodomain becomes highly compact upon complexation with the Wnt antagonist Dkk1, suggesting a potential role for the ectodomain conformational change in the regulation of receptor oligomerization and signaling. PMID: 28052259
- Our results suggest that measurement of DKK-1 combined with DKK-1 autoantibodies is a potentially valuable tool for the early detection of ESCC. PMID: 26988995
- TET1 potently inhibited canonical Wnt/beta-catenin signaling by demethylating and upregulating two upstream antagonists of this pathway, SFRP2 and DKK1, which was associated with inhibition of EMT and cancer cell metastasis. PMID: 28851501
- This is the first study demonstrating a link between B cell depletion and skin Dkk-1 upregulation in patients with systemic sclerosis PMID: 27208972
- We found significantly increased levels of DKK-1 in patients with acute CO poisoning, compared to healthy controls. High levels of serum DKK-1 in patients were significantly correlated with severe neurological symptoms. PMID: 27353797
- DKK1 prevents lung metastasis of breast cancer cells but promotes breast-to-bone metastasis. PMID: 28892080
- Structure of the dual-mode Wnt regulator Kremen1 and insight into ternary complex formation with LRP6 and DKK1 have been presented. PMID: 27524201
- Expression of Cancer stem cells-associated DKK1 mRNA might be an unfavorable prognostic marker for patients with hepatocellular carcinoma. PMID: 28870909
- In chronic kidney disease, serum levels of the Wnt inhibitors DKK1 and sclerostin are unrelated, indicating different sites of origin and/ or different regulatory mechanisms. Sclerostin, as opposed to DKK1, may qualify as a biomarker of chronic kidney disease-mineral and bone and mineral disorder (CKD-MBD), particularly in dialysis patients. PMID: 28493902
- endogenous expression of the WNT antagonists DKK1 and FRZB is necessary for multiple steps during chondrogenesis PMID: 27733096
- The expression level of DKK1 in serum was increased in breast cancer patients, suggesting that serum expression level of DKK1 could be a useful biomarker in breast cancer. PMID: 28248066
- Results show that the expression of DKK1 is elevated in human HBV-related hepatocellular carcinoma tissues. PMID: 28419472
- DKK-1 and PDCD5 can be independent predictors of overall survival in patients suffering from chondrosarcoma. PMID: 27255549
- In patients with hepatocellular carcinoma serum DKK1 level correlated with tumor size, consequently, patients with bigger tumors tend to have higher levels of DKK1. PMID: 27161933
- Our results indicate that DKK1 and SFRP1 may be potentially useful biomarkers for evaluating the beneficial effects of long-term exercise on physical fitness and metabolism as well as the prognosis of patients with cancer. PMID: 28178355
- Dickkopf-1 and sclerostin were never correlated with each other or with bone turnover markers patients with Paget's disease of bone PMID: 28054306
- these findings suggest that DKK1 induces the occurrence of epithelial-mesenchymal transition and promotes migration and invasion in non-small cell lung cancer cells. Mechanically, b-catenin plays a vital role in DKK1-induced non-small cell lung cancer cell migration and invasion, and DKK1 inhibits the phosphorylation of b-catenin, resulting in the increased nuclear localization of b-catenin. PMID: 28677426
- Results suggest that miR-107 is likely to inhibit the occurrence and development of osteosarcoma by down-regulating Dkk-1 via the Wnt/beta-catenin signaling pathway. PMID: 28682874
- Recombinant Dkk1 suppresses beta-catenin target genes in myeloid-derived suppressor cells (MDSCs) from mice and humans and anti-Dkk1 loses its antitumor effects in mice lacking beta-catenin in myeloid cells or after depletion of MDSCs, demonstrating that Dkk1 directly targets MDSCs. PMID: 27045006
- Luciferase-fused 3' untranslated region of human Dickkopf1 activity was highly upregulated in A1CF-overexpressed MCF7 cells, but this upregulation can be inhibited by mutating conserved binding motifs of Dickkopf1 3' untranslated region. A1CF played a crucial role in cell migration and survival through affecting 3' untranslated region of Dickkopf1 to upregulate its expression in MCF7 cells. PMID: 28639893
- Circulating sclerostin and Dickkopf-1 levels do not change across the menstrual cycle and do not demonstrate any relationship with estradiol in premenopausal women. PMID: 27601021
- Results found miR-203 and DKK1 to be expressed at differential levels in cisplatin-sensitive and -insensitive lung adenocarcinoma tissues; there was also a negative correlation between them. PMID: 28350100
- Serum levels of OPG and DKK-1 were measured in 97 rheumatoid arthritis patients who were treated according to a treat-to-target strategy (T2T) aimed at remission (DAS28<2.6). PMID: 27911913
- DKK1 can promote vasculogenic mimicry formation via induction of the expression of epithelial-mesenchymal transition and cancer stem-like cell-related proteins. PMID: 27240974
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亞細(xì)胞定位:Secreted.
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蛋白家族:Dickkopf family
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組織特異性:Placenta.
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