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  4. Recombinant Human Docking protein 1 (DOK1)

Recombinant Human Docking protein 1 (DOK1)

  • 中文名稱(chēng)：
    人DOK1重組蛋白
  • 貨號(hào)：
    CSB-EP858723HU
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    DOK1
  • Uniprot No.：
  • 別名：
    Docking protein 1 62kD ; Docking protein 1; DOK 1; DOK1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    E.coli
  • 分子量：
    68.4kDa
  • 表達(dá)區(qū)域：
    1-481aa
  • 氨基酸序列
    MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • 基因功能參考文獻(xiàn)：
    1. Taken together, these results indicate that ATRA-enhanced expression of DOK1 activates PPARgamma leading to inhibition of cell proliferation and enhancement of cell apoptosis in MCF-7 cell. PMID: 28396148
    2. DOK1 was identified as a prognostic factor for non-metastatic CRC, and, via its drugability by PPARgamma-agonist, may constitute a potential target for future cancer treatments. PMID: 27428427
    3. DOK3 expression was not altered much in HTLV-1-infected T cells. PMID: 27265473
    4. Results indicate that hypermethylation of tumor suppressor protein RASSF1A and docking protein 1 (DOK1) contributes to hepatocarcinogenesis and is associated to clinicopathological characteristics. PMID: 27078152
    5. Data show that residues Ser745 and Ser756 in the integrin beta2 tail, which are adjacent to the NxxF motif, are required for docking protein 1, docking protein 1, 62kDa (downstream of tyrosine kinase 1) (Dok1) interaction. PMID: 26108885
    6. results support a model in which Dok1 phosphorylation normally suppresses localized Ras pathway activity in Crk-transformed cells via recruitment and/or activation of RasGAP PMID: 25043303
    7. Data implicate existence of alternate conformational states around the ligand binding pocket of the PTB domain of phosphoprotien Dok1 either in the native or in the near native conditions. PMID: 24587391
    8. Deregulation of DOK1 gene expression by EBV and novel insights into the regulation of the DOK1 tumor suppressor in viral-related carcinogenesis. PMID: 24809689
    9. point mutations in DOK1 and DOK2 genes are detected with low frequency in chronic myelomonocytic leukemia but may have consequences for the function of the DOK2 PTB domain PMID: 25252871
    10. A crucial role for DOK1 in the regulation of PDGF-BB-mediated tumour cell motility through a p130Cas-Rap1 signalling pathway. PMID: 24762811
    11. Taken together, these results reveal that Dok1 and Dok2 proteins are involved in an intrinsic negative feedback loop downstream of natural killer-cell-activating receptors in mouse and human. PMID: 24963146
    12. BRK has a role in targeting Dok1 for ubiquitin-mediated proteasomal degradation and in promoting cell proliferation and migration PMID: 24523872
    13. The unique N-terminal region of SRMS regulates enzymatic activity and phosphorylation of its novel substrate docking protein 1. PMID: 23822091
    14. DNA methylation of the DOK1 core promoter region found in head and neck cancer cell lines hampered the recruitment of E2F1 to the DOK1 promoter and compromised DOK1 expression. PMID: 23028047
    15. Studies demonstrate DOK-1 regulates allergen-induced Th2 immune responses by selective stimulation and inhibition of STAT-4 and STAT-6 signaling pathways, respectively. PMID: 22514638
    16. hypermethylation of DOK1 is a potentially critical event in human carcinogenesis. PMID: 21796618
    17. These findings are suggestive for a possible tumor suppressor role of DOK1 in epithelial ovarian cancer. PMID: 21856257
    18. Cav1 cooperated with the endogenous Ras/MAPK inhibitor docking protein 1 (Dok1) to promote the ligand-dependent transcriptional activity of PPARgamma and to inhibit cell proliferation PMID: 21690289
    19. these data support a model in which proteasome- mediated degradation of Dok-1 is an important contributive step toward tumor development and/or progression driven by OTKs PMID: 21536658
    20. Dok1 negatively regulates Dok2-mediated CD200R signaling through the recruitment of CrkL. PMID: 21078907
    21. Identification of DOK genes as lung tumor suppressors. PMID: 20139980
    22. The novel platelet adapter Dok-1 is tyrosine phosphorylated in an Src kinase-independent manner downstream of alphaIIbbeta3 in human platelets, leading to an interaction with Grb2 and SHIP-1. PMID: 19682241
    23. Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling. PMID: 11825908
    24. Dok-1 acts as an adaptor protein that links the activin receptors with the Smads, suggesting a novel function for Dok-1 in activin signaling leading to B-cell apoptosis. PMID: 11927552
    25. DOK1 and DOK2 interact with the Tec protein tyrosine kinase. PMID: 14647425
    26. Dok-1 plays an important role in SDF-1alpha/CXCL12-induced chemotaxis in T cells. PMID: 15345598
    27. This result indicates that germline mutations in Dok1 are unlikely to cause an inherited predisposition to CLL. PMID: 15541476
    28. DOK1 mediates SHP-2/beta3 association in response to IGF-I thereby mediating the effect of integrin ligand occupancy on IGF-IR-linked signaling in smooth muscle cells. PMID: 15546884
    29. IKKbeta phosphorylates Dok1 S(439)S(443) and S(446)S(450) after TNF-alpha, IL-1, or gamma-radiation and implicate the critical Dok1 serines in Dok1 effects after tyrosine kinase activation PMID: 15574499
    30. Phosphotyrosine-binding mediated oligomerization of Dok-1 and Dok-2 represents an essential step for Dok phosphorylation and function. PMID: 16177091
    31. Data show that Dok1 expression and structure are affected in a subset of Burkitt's lymphoma samples, suggesting its possible role in this type of cancer. PMID: 16338067
    32. These data suggest a mechanistic basis for the inhibitory effect of Dok-1 on growth factor-induced mitogenesis and its role as a tumor suppressor. PMID: 16537894
    33. The data provide evidence that DOK1 protein has a role in regulating cell proliferation and differentiation and is positive regulators of the MAPK signaling pathway in this context. PMID: 16823827
    34. results demonstrate differential modes of regulation of Dok1 and Dok2 in platelets, and raise the possibility that Dok2 plays an important role in integrin outside-in signaling through a physical and functional interaction with integrin alphaIIbbeta3 PMID: 17092301
    35. Upon phosphorylation of Tyr 747 in the beta3 integrin tail, however, Dok1 then binds much more strongly than talin. PMID: 18156175
    36. These results suggest that engagement of different adaptor proteins by Ret results in very different downstream signaling and functions within neurons and that Dok recruitment leads to a rapid receptor relocation and formation of microspikes. PMID: 18353552
    37. Dok-1/Dok-2 pleckstrin homology domains bind in vitro to the rare phosphoinositide species, phosphatidylinositol 5-phosphate PMID: 19299694
    38. CD45 recruits adaptor DOK-1 to the proximal plasma membrane to serve as a downstream effector, resulting in negative regulation of the JAK/STAT signaling pathway. PMID: 19481264
    39. Results identified an N-terminally truncated isoform of human Dok-1 with N-terminal acetylation as seen in the wild-type. PMID: 19481542

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  • 亞細(xì)胞定位：
    [Isoform 1]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm, perinuclear region.
  • 蛋白家族：
    DOK family, Type A subfamily
  • 組織特異性：
    Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 2990

    OMIM: 602919

    KEGG: hsa:1796

    STRING: 9606.ENSP00000233668

    UniGene: Hs.103854