Recombinant Human Elongation factor 2 (EEF2)
-
中文名稱:人EEF2重組蛋白
-
貨號(hào):CSB-BP007434HU
-
規(guī)格:¥2832
-
圖片:
-
其他:
產(chǎn)品詳情
-
純度:Greater than 90% as determined by SDS-PAGE.
-
生物活性:Not Test
-
基因名:
-
Uniprot No.:
-
別名:EF-2
-
種屬:Homo sapiens (Human)
-
蛋白長(zhǎng)度:Full Length of Mature Protein
-
來源:Baculovirus
-
分子量:96.2 kDa
-
表達(dá)區(qū)域:2-858aa
-
氨基酸序列VNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. -
蛋白標(biāo)簽:N-terminal 6xHis-tagged
-
產(chǎn)品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
-
復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
-
儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
-
保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
-
Datasheet & COA:Please contact us to get it.
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能:Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
-
基因功能參考文獻(xiàn):
- our study indicates that expression of eEF2 protein is a potential biomarker for evaluating prostate cancer PMID: 28913610
- eEF2 may be activated in a positive feedback cycle through inactivation of eEF2K via the PI3K/Akt/mTOR pathway. PMID: 30060827
- the direct interaction of AKT2 and EF2 was found to be dynamically regulated in embryonic rat cardiomyocytes PMID: 23823123
- These results suggest that binding of eEF2 to the ribosome alters its conformation, resulting in a weakened affinity of eIF5A and impairment of this interplay compromises cell growth due to translation elongation defects. PMID: 27115996
- nuclear localization of active eEF-2 depends upon its interaction with p53, as cells lacking p53 contain less active eEF-2 in the nuclear compartment PMID: 23542375
- High serum eukaryotic elongation factor 2 level is associated with non-small cell lung cancer. PMID: 24377563
- Results indicated that the eEF2 gene is overexpressed in the majority of several types of cancers and plays an oncogenic role in cancer cell growth. PMID: 24589652
- The expression levels of three putative HA-regulated proteins (TALDO, ANXA1 and EF2) in control, H2O2-, HA- and HA/H2O2-treated OA chondrocytes were verified by Western blotting and the results indeed support the notion that HA acts in anti-oxidation PMID: 24480285
- Thiopental inhibits global protein synthesis by repression of eukaryotic elongation factor 2 and protects from hypoxic neuronal cell death. PMID: 24167567
- A single amino acid substitution in eukaryotic elongation factor 2 co-segregates with the disease phenotype of spinocerebellar ataxia 26. PMID: 23001565
- EEF2 phosphorylation by cyclin A-cyclin-dependent kinase 2 (CDK2) on a novel site, serine 595 (S595), directly regulates T56 phosphorylation by eEF2K. PMID: 23184662
- Burn induces prolonged activation of eEF2K and eEF2 in pediatric patients. PMID: 22269896
- PHD2 modulated eEF2 activity and protein translation under acute hypoxia. PMID: 22308030
- NH125 induces eEF2 phosphorylation (peEF2) through multiple pathways in cancer cells PMID: 22020937
- Training in the fasted state, compared with identical training with ample carbohydrate intake, facilitates post-exercise dephosphorylation of eEF2. PMID: 21132439
- results suggest that eEF2 is an anti-apoptotic marker in lung adenocarcinoma PMID: 21554491
- eEF-2 is activated by both lithium and GSK-3, whereas, lithium treatment and inhibition of GSK-3 have opposing effects on eEF-2. PMID: 20708687
- melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
- inhibition of protein synthesis under high pressure occurs independent of the phosphorylation of eEF-2 PMID: 15534876
- Levels of p-eEF2K were significantly increased, and total eEF2 significantly decreased in Alzheimer disease PMID: 16098202
- Data show that overproduction of elongation factor 2 blocks HIV-1 viral protein R-induced cell death both in fission yeast and human cells. PMID: 16520893
- Hypoxia inhibits protein synthesis through a 4EBP1 and EEF2 pathway controlled by mTOR and unbcoupled in breast cancer cells. PMID: 16648488
- Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type. PMID: 19036825
- Overexpressed eEF2 in gastrointestinal cancers promoted G2/M progression and enhanced their cell growth in vitro and in vivo. PMID: 19360331
顯示更多
收起更多
-
相關(guān)疾病:Spinocerebellar ataxia 26 (SCA26)
-
亞細(xì)胞定位:Cytoplasm. Nucleus.
-
蛋白家族:TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-G/EF-2 subfamily
-
數(shù)據(jù)庫鏈接:
Most popular with customers
-
Recombinant Human Tyrosine-protein kinase Mer (MERTK), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Melanoma-associated antigen 4 (MAGEA4) (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Rat Microtubule-associated protein tau (Mapt) (Active)
Express system: Mammalian cell
Species: Rattus norvegicus (Rat)
-
Recombinant Human Claudin-6 (CLDN6)-VLPs (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Myosin regulatory light polypeptide 9 (MYL9) (Active)
Express system: Yeast
Species: Homo sapiens (Human)
-
Recombinant Mouse Gastric inhibitory polypeptide receptor (Gipr), partial (Active)
Express system: Mammalian cell
Species: Mus musculus (Mouse)
-
Recombinant Rat Gastric inhibitory polypeptide receptor (Gipr), partial (Active)
Express system: Mammalian cell
Species: Rattus norvegicus (Rat)