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  4. Recombinant Human Endonuclease G, mitochondrial (ENDOG)

Recombinant Human Endonuclease G, mitochondrial (ENDOG)

  • 中文名稱：
    人ENDOG重組蛋白
  • 貨號(hào)：
    CSB-YP622760HU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    人ENDOG重組蛋白
  • 貨號(hào)：
    CSB-EP622760HU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    人ENDOG重組蛋白
  • 貨號(hào)：
    CSB-EP622760HU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人ENDOG重組蛋白
  • 貨號(hào)：
    CSB-BP622760HU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人ENDOG重組蛋白
  • 貨號(hào)：
    CSB-MP622760HU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ENDOG; Endonuclease G; mitochondrial; Endo G; EC 3.1.30.-
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    49-297
  • 氨基酸序列
    AE LPPVPGGPRG PGELAKYGLP GLAQLKSRES YVLCYDPRTR GALWVVEQLR PERLRGDGDR RECDFREDDS VHAYHRATNA DYRGSGFDRG HLAAAANHRW SQKAMDDTFY LSNVAPQVPH LNQNAWNNLE KYSRSLTRSY QNVYVCTGPL FLPRTEADGK SYVKYQVIGK NHVAVPTHFF KVLILEAAGG QIELRTYVMP NAPVDEAIPL ERFLVPIESI ERASGLLFVP NILARAGSLK AITAGSK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four-stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation. Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication.
  • 基因功能參考文獻(xiàn)：
    1. These data indicated the participation of EndoG in alternative mRNA splicing of the telomerase catalytic subunit hTERT, regulation of telomerase activity and determination of cell fate. PMID: 28886883
    2. Galectin-3-induced cell death in HIV-1-infected macrophages is most likely related to the translocation of Endo G from the cytoplasm to the nucleus. PMID: 27981746
    3. ndoG digests long non-coding RNA and produces 47-mer RNA oligonucleotide complementary to hTERT pre-mRNA exon 8 and intron 8 junction place. Interaction of 47-mer RNA oligonucleotide and hTERT pre-mRNA causes alternative splicing. PMID: 27797329
    4. Overexpression of EndoG in CD4+ T cells downregulated the expression of the active full-length hTERT variant and upregulated the inactive alternatively spliced variant. PMID: 28320284
    5. Overexpression of EndoG in capital ES, Cyrillicsmall a, Cyrilliccapital ES, Cyrillicsmall o, Cyrillic-2 cells downregulated the expression of active full-length hTERT variant and upregulated non-active spliced variant. PMID: 27420614
    6. EndoG is not a mediator of exogenous DNA clearance, but in non-physiological circumstances, it may nonspecifically cleave intracellular DNA regardless of its origin. PMID: 27260396
    7. These findings suggest that non-viral DNA vectors are also substrates for EndoG in its role in homologous recombination PMID: 27239850
    8. our study established a link between Endo G and mitochondrial function during cardiac hypertrophy PMID: 26492643
    9. Results show that BNIP3 interacts with the voltage-dependent anion channel (VDAC) to directly induce mitochondrial release and nuclear translocation of EndoG. PMID: 25436615
    10. Endonuclease G (EndoG), an apoptotic nuclease, as an essential factor for MLLbcr-specific DNA recombination after induction of replication stress. PMID: 25132265
    11. Ethyl gallate induces apoptosis of HL-60 cells by promoting the expression of caspases-8, -9, -3, apoptosis-inducing factor and endonuclease G. PMID: 23109891
    12. Data suggest that endonuclease G (EndoG) inhibitors have the potential to be utilized for amelioration of cell injuries in which participation of EndoG is essential. PMID: 25401220
    13. study demonstrate EndoG interacts with cellular Inhibitor of Apoptosis Protein 1 (cIAP1); results indicate IAPs interact and ubiquitinate EndoG via K63-mediated isopeptide linkages without affecting EndoG levels and EndoG-mediated cell death, suggesting EndoG ubiquitination by IAPs may serve as a regulatory signal independent of proteasomal degradation PMID: 25139236
    14. Using gene reporter assays, we show that promoter variations in 11 intrinsic apoptosis genes, including ADPRT, APAF1, BCL2, BAD, BID, MCL1, BIRC4, BCL2L1, ENDOG, YWHAB, and YWHAQ, influence promoter activity in an allele-specific manner. PMID: 24038028
    15. Data indicate that older muscles showed a 3-fold greater fraction of endonuclease G (a mitochondrial proapoptotic factor)-positive myonuclei. PMID: 24371120
    16. CHIP overexpression reduces EndoG levels, and results in reduced or no oxidative stress-induced cell death in cultured cancer cells. PMID: 23764847
    17. Endonuclease G mediates alpha-synuclein cytotoxicity during Parkinson's disease. PMID: 24129513
    18. These data indicate that HSV-1 UL12.5 deploys cellular proteins, including ENDOG and EXOG, to destroy mtDNA and contribute to a growing body of literature highlighting roles for ENDOG and EXOG in mtDNA maintenance. PMID: 23986585
    19. Conclude that EndoG and TOPO2a may actively participate in apoptotic chromatin degradation. PMID: 22160858
    20. Benzyl isothiocyanate induces apoptosis in DU 145 cells through the release of AIF and Endo G from the mitochondria and also promotes caspase-3 activation. PMID: 21206973
    21. ENDOG might contribute to cancer pathogenesis by expressional alterations, but not by somatic mutations PMID: 21080888
    22. When EndoG gene expression was downregulated by lentiviral shRNA vectors, we found a significant reduction in the replicative life span and a corresponding increase in cell death. PMID: 20211237
    23. nuclear translocation of apoptosis-inducing factor and endonuclease G play a crucial role in (-)-Epigallocatechin-3-gallate -induced apoptosis of human laryngeal epidermoid carcinoma Hep2 cells PMID: 19781850
    24. examination of submitochondrial localization and its ability to cleave R-loops in order to clarify role in mtDNA replication PMID: 12444964
    25. endonuclease G and apoptosis-inducing factor are relocated and have roles in calcium induced signaling and oxidative stress-related impairment of mitochondria PMID: 15182854
    26. EndoG forms complexes with AIF and FEN-1 but not with PCNA. Heat shock proteins 70 interact with EndoG and are involved in the regulation of its activity. PMID: 16133872
    27. Our findings suggest that the presence of EndoG in non-invasive breast cancer cells determines their sensitivity to apoptosis, which may be taken into consideration for developing the chemotherapeutic strategy for cancer treatment. PMID: 17046751
    28. Hypochlorous acid induced Bax-dependent mitochondrial permeability which led to cell death without caspase activity by processes involving AIF/EndoG-dependent pathways. PMID: 17107772
    29. study of cellular localization of the endonuclease G, AIF & AMID during apoptosis using bioinformatics and image analysis PMID: 17347867
    30. EndoG inactivation by loss of expression may not occur in colorectal and gastric cancers. Rather, neo-expression of EndoG may play a role in both colorectal and gastric tumorigenesis. PMID: 18705403
    31. data showed that loss of EndoG expression is a feature of hepatocellular carcinomas PMID: 18754329
    32. These results suggest that Bim, Bcl-xL, FAK and endonuclease G are involved in safingol-induced apoptosis of detached oral squamous cell carcinoma. PMID: 19199036
    33. Results suggest that human Endonuclease G shares a similar catalytic mechanism with nuclease A from Anabaena. PMID: 19272175

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  • 亞細(xì)胞定位：
    Mitochondrion.
  • 蛋白家族：
    DNA/RNA non-specific endonuclease family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 3346

    OMIM: 600440

    KEGG: hsa:2021

    STRING: 9606.ENSP00000361725

    UniGene: Hs.745161