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  4. Recombinant Human Estrogen receptor beta (ESR2), partial

Recombinant Human Estrogen receptor beta (ESR2), partial

In Stock
  • 中文名稱：
    人ESR2重組蛋白
  • 貨號(hào)：
    CSB-EP007831HU1
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    ＞85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    ER BETA; ER-beta; Erb; ESR B; ESR BETA; ESR2; ESR2_HUMAN; ESRB; ESTRB; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; estrogen receptor 2 (ER beta); Estrogen receptor 2; estrogen receptor beta 4; Estrogen receptor beta; NR3A2; Nuclear receptor subfamily 3 group A member 2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Partial of Isoform 3
  • 來(lái)源：
    E.coli
  • 分子量：
    39.8kDa
  • 表達(dá)區(qū)域：
    2-323AA of Isoform 3
  • 氨基酸序列
    DIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGMRGNA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner.; Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.
  • 基因功能參考文獻(xiàn)：
    1. An association was found between the rs1256032 polymorphism of estrogen receptor beta gene and reduced risk in a Mexican population of developing type 2 diabetes. PMID: 29666032
    2. We identified a homozygous ESR2 variant, c.541_543del p.(Asn181del) in the highly conserved DNA-binding domain of ER-beta in an individual with syndromic 46,XY DSD. Two additional heterozygous missense variants, c.251G>T p.(Gly84Val) and c.1277T>G p.(Leu426Arg), located in the N-terminus and the ligand-binding domain of ER-beta, were found in unrelated, nonsyndromic 46,XY DSD cases. PMID: 29261182
    3. therapeutic activation of ERbeta elicits potent anticancer effects in Triple-negative breast cancer through the induction of a family of secreted proteins known as the cystatins, which function to inhibit canonical TGFbeta signaling and suppress metastatic phenotypes both in vitro and in vivo. PMID: 30257941
    4. ESR2 polymorphisms demonstrate minimal influence on Bone mineral density changes in women. PMID: 29320918
    5. Low ESR2 expression is associated with Tumor Progression in Colorectal Cancer with Familial Adenomatous Polyposis and Sporadic Polyps. PMID: 28681123
    6. The possible role of ESR2 G1730A variant as the risk factor of MI in a young age. PMID: 30036658
    7. TCF21 modulates Steroidogenic factor-1 and estrogen receptor beta expression through the recruitment of USF2 in endometriotic stromal cells. PMID: 30018006
    8. High phosphorylated ESR2 expression is associated with pancreatic ductal adenocarcinoma. PMID: 30046904
    9. FSHR rs6165, not ESR2 rs4986938, could be suggested as a candidate marker to predict poor ovarian response. PMID: 29036809
    10. There was a significant association between ERbeta RsaI and postmenopausal osteoporosis risk in both overall and Asian populations PMID: 29458346
    11. Estrogen receptor beta1 activation accelerates resistance to epidermal growth factor receptor-tyrosine kinase inhibitors in non-small cell lung cancer. PMID: 29328407
    12. Increased cytoplasmic ERbeta1 and nuclear ERbeta2 expression is associated with worse cancer specific outcomes following radical prostatectomy. These findings suggest that tumor ERbeta1 and ERbeta2 staining patterns provide prognostic information on patients treated with radical prostatectomy. PMID: 26804755
    13. ESR2 single nucleotide polymorphism is associated with poor ovarian response in Egyptian women undergoing IVF procedure. PMID: 28825151
    14. ERbeta expression conferred cell sensitivity, as patient-derived AML cells with high levels of ERb were sensitive, whereas cells with low ERb were insensitive to diosmetin. Knockdown of ERb confirmed resistance, whereas overexpression enhanced sensitivity to diosmetin, which was demonstrated to be mediated by reactive oxygen species signaling. PMID: 28835383
    15. Data report for the first time that ERbeta inversely regulates miR-10b and miR-145, which are critical modulators of functional properties, epithelial to mesenchymal transition and extracellular matrix composition of breast cancer cells. ERbeta together with miR-10b and miR-145, are key players for the initiation of breast cancer cell aggressiveness. PMID: 28797712
    16. the mRNA expression levels of ERbeta isoforms are downregulated in sporadic colorectal cancer and in FAP individuals. T3/T4 tumours also presented decreased expression of ERbeta. Additionally, the expression levels of ERbeta1 and ERbeta5 were associated with the probability of disease-free survival. PMID: 29132333
    17. factors that regulate the alternative splicing of ERs remain unknown. We demonstrate novel evidence supporting a potential biological feedback loop where 17beta-estradiol regulates the RNA-binding protein Nova1, which, in turn, regulates the alternative splicing of ERbeta PMID: 29031089
    18. A dual role of the association between AGO2 and ERbeta in luminal-like breast cancer cells in the nucleus and the cytoplasm, for the regulation of gene expression at both the transcriptional and post-transcriptional level. PMID: 29017520
    19. We found higher nuclear (n)ERalpha and nERbeta5 and lower cytoplasmic (c)ERalpha expression in advanced ovarian cancers. Lower ERbeta1 expression was also detected in high grade cancers. Loss of nERalpha and cERbeta2 expression were observed in clear cell histological subtypes. Higher nERbeta5 and lower cERbeta5 expression were associated with serous/clear cell subtypes, poor disease-free and overall survival. PMID: 28859612
    20. We found little overall difference in the DNA methylation of the ESR2 5'-flanking region in individuals with autism compared with normal individuals. PMID: 28299627
    21. our data reveal a novel mechanism for ERbeta's anti-proliferative and pro-apoptotic effects in breast cancer cells involving p53 and epigenetic changes in histone methylation that underlie gene regulation of these cellular activities. PMID: 28577282
    22. loss of ERbeta function acts in collaboration with p53 inactivation to induce early onset of mammary tumors with spindle cell morphology and more basal-like characteristics. PMID: 28673316
    23. Low levels of ESR2 is associated with metastasis in triple-negative breast cancer. PMID: 28583190
    24. Findings from the systematic review and meta-analysis suggest that rs1256120 of ESR2 is unlikely to be a predisposing or disease-modifying genetic risk factor for adolescent idiopathic scoliosis. PMID: 27755497
    25. High concentrations of DINCH urinary metabolites activate human ESR2 receptors. PMID: 29421333
    26. Tumor ERbeta1 expression was available for 911 breast cancer patients and was not associated with ESR2 genotypes. High tumor ERbeta1 expression is a marker of good prognosis in breast cancer, especially among chemotherapy-treated patients, but not in endocrine therapy-treated patients. PMID: 27810901
    27. Targeting ERbeta with selective agonists might affect Hodgkins lymphoma cell proliferation and tumor growth via a mechanism that brings into play DRAM2-dependent autophagic cascade. PMID: 28052027
    28. ERbeta might be the target protein of arctigenin responsible for inhibition of mTORC1 activation and resultant prevention of Th17 cell differentiation and colitis development. PMID: 27863380
    29. Data suggest that the ERalpha-H19-BIK signaling axis plays an important role in promoting breast cancer cell chemoresistance. PMID: 27845892
    30. fulvestrant up-regulated ERbeta in ERalpha+/ERbeta+ breast cancer and in triple negative ERbeta+ breast cancers (ERalpha-/ERbeta+) PMID: 27486755
    31. ERbeta plays a crucial role in the cell behavior and extracellular matrix composition of the highly aggressive MDA-MB-231 cells. PMID: 27179695
    32. Study provides evidence for a critical role of ESR2 in radio-resistance of in head and neck squamous cell carcinoma, and that SMR3A might serve as a surrogate marker for active ESR2 signaling. PMID: 28166815
    33. This study demonstrated that ESR2 but not ESR1 minor alleles were associated with lower cognitive performance in elderly women with an indication of a gene-gene interaction with APOEepsilon4. And also found indications for gene-environment interactions of ESR2 with traffic-related air pollution exposure on cognitive performance. PMID: 27629499
    34. LY500307 is a highly selective estrogen receptor beta agonist, which loses its selectivity at high dose and leads to undesirable suppression of total testosterone concentration. PMID: 27136911
    35. ERbeta ubiquitination, protein turnover, and inhibition of breast cancer is regulated by tyrosine phosphorylation PMID: 27323858
    36. ERbeta in both normal and cancerous colon epithelial cells upregulates miRNA-205, which subsequently reduces PROX1 through direct interaction with its 3'UTR PMID: 27283988
    37. Membrane estrogen receptor beta and GPER signaling mediate cellular responses to environmentally relevant concentrations of CdCl2 and NaAsO2 in lung adenocarcinoma cells. PMID: 27071941
    38. C-Jun and CREB are recruited to ESR2 or CYP19A1 promoter by IGF-I stimulation. PMID: 26899323
    39. increases in ERbeta and p53 immunoreactivity were significantly correlated with the incidence of metastasis and/or recurrence in endometrial endometrioid carcinoma PMID: 29190668
    40. Low estrogen receptor beta is associated with triple-negative breast cancer. PMID: 26871946
    41. The variation at rs10144225 in ESR2 was associated with salt-sensitive blood pressure (SSBP) in premenopausal women (estrogen-replete) and not in men or postmenopausal women (estrogen-deplete). Inappropriate aldosterone levels on a liberal salt diet may mediate the SSBP. PMID: 28938457
    42. Data suggest that 17-beta-estradiol/ESR2 signaling counteracts TNFA-induced, inflammation-mediated apoptosis of vascular endothelial cells in a mechanism that requires NOTCH1-mediated Akt phosphorylation. (ESR2 = estrogen receptor 2; TNFA = tumor necrosis factor-alpha; NOTCH1 = Notch homolog 1, translocation-associated protein) PMID: 28893903
    43. Expression of ERbeta and p53 significantly correlated with age and advanced cancer stages, and the p53-negative EOGC was associated with favorable outcomes. PMID: 27781410
    44. Our data suggest that oestrogens stimulated osteogenic differentiation in human dental pulp cells and that this action is mediated principally through the ERbeta isoform PMID: 29031686
    45. ESR2 may modify the interaction between negative life events and MDD in the Chinese Han menopausal females. PMID: 27814959
    46. Results show that ERb is upregulated in bladder malignant tissue, and its expression is associated with muscle-invasive disease. PMID: 27324053
    47. estrogen may play a role in androgen-independent prostate cancer cell proliferation through a novel pathway, involving ERbeta-mediated activation of beta-catenin. PMID: 27107935
    48. ERbeta immunoreactivity was markedly present in all the cases of Merkel cell carcinoma examined. significant correlation with Ki-67 labeling index and Bcl-2 score suggest that ERbeta could be associated with regulation of both cell proliferation and apoptosis in MCCs. PMID: 27343835
    49. Germline ESR2 mutation is associated with medullary thyroid carcinoma. PMID: 26945007
    50. Exposure to the combination of 100 muM genistein and 10 nM calcitriol reduced the number of proliferative cells to control levels, increased ERb and VDR expression, and reduced extracellular acidification (40%) as well as respiratory activity (70%), primarily in MG-63 cells..strong overexpression SGPL1, which irreversibly degrades sphingosine-1-phosphate thereby, generating ethanolamine PMID: 28125641

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR3 subfamily
  • 組織特異性：
    [Isoform 1]: Expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, b
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 3468

    OMIM: 601663

    KEGG: hsa:2100

    STRING: 9606.ENSP00000343925

    UniGene: Hs.660607