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  4. Recombinant Human Extracellular calcium-sensing receptor (CASR), partial

Recombinant Human Extracellular calcium-sensing receptor (CASR), partial

Unavailable
  • 中文名稱：
    Recombinant Human Extracellular calcium-sensing receptor(CASR),partial,Yeast
  • 貨號：
    CSB-YP004558HU
  • 規(guī)格：
    ￥1500
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    Ca sensing receptor; Ca2+ sensing receptor 1; Ca2+ sensing receptor; Calcium sensing receptor; CAR; CaSR; CASR_HUMAN; EIG8; Extracellular calcium sensing receptor; Extracellular calcium sensing receptor [Precursor]; Extracellular calcium-sensing receptor; FHH; FIH; GPRC2A; HHC; HHC1; Hypocalciuric hypercalcemia 1; Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism; MGC138441; NSHPT; Parathyroid Ca(2+) sensing receptor 1; Parathyroid Cell calcium sensing receptor; Parathyroid Cell calcium-sensing receptor; PCAR 1; PCaR1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Extracellular Domain
  • 來源：
    Yeast
  • 分子量：
    68.6kDa
  • 表達(dá)區(qū)域：
    20-612aa
  • 氨基酸序列
    YGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFLRGHEESGDRFSNSSTAFRPLCTGDENISSVETPYIDYTHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSCIAKEIEFLSWTEPF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis. Senses fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. The G-protein-coupled receptor activity is activated by a co-agonist mechanism: aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation.
  • 基因功能參考文獻(xiàn)：
    1. Performed cytogenetic analysis in 23 patients with Sagliker syndrome; found base alterations and deletions in exons 2 and 3 of the calcium sensing receptor (CaSR) gene. PMID: 28263480
    2. Based on these findings, ischemia/reperfusion-induced MCPIP1 expression regulates the migration and apoptosis of human vascular endothelial cells via HMGB1 and CaSR, respectively. PMID: 29379093
    3. expressions of p27(Kip1) and CaSR were decreased in primary hyperparathyroidism patients PMID: 29589297
    4. Study presents the novel concept that CaSR activation stimulates autophagy in preadipocytes, which in turn mediates the elevation of TNFalpha production. PMID: 30251678
    5. The identification of the activation of CaSR-mediated protective pathway in renal cells sheds light on a possible cellular protective mechanism against Cd-induced kidney injury. PMID: 29348484
    6. These findings suggest an inhibitory role for CaSR in endometrial cancer. Therefore, reduced CaSR expression may be a suitable explanation and valuable predictor for endometrial cancer progression. PMID: 29348629
    7. We found that subjects carrying the G allele of rs6776158 (AG and GG) had significantly higher risk of nephrolithiasis compared to the AA genotype.Our results indicate that rs6776158 polymorphism that might elevate the risk of nephrolithiasis in the Chinese population. PMID: 30407299
    8. The variant allele of CASR rs1801725 solely and together with the variant allele of rs7652589 increases risk of more advanced secondary hyperparathyroidism. PMID: 29763933
    9. findings confirm the expression of CaSR in human BM-derived MSCs and unravel a prominent role for the interplay between CaSR and PTH1R in regulating MSC fate and the choice of pathway for bone formation. PMID: 29915064
    10. Genetic polymorphism of calcium-sensing receptor is associated with breast cancer. PMID: 29387985
    11. The low prevalence of CaSR autoantibodies indicate a very low level of subclinical parathyroid autoimmunity in APS types 2, 3 and 4. PMID: 28941288
    12. CaSR Arg990Gly polymorphism is associated with the risk of nephrolithiasis development in a Chinese population. PMID: 28609763
    13. TRPC1 is a primary candidate in forming SOCC that stimulates CaSRinduced SOCE and NO production in HUVECs PMID: 28791397
    14. The c.2195A>G, p.(Asn732Ser) mutation in exon 7 of the CaSR gene leads to hypocalcaemia, and has not been reported before in the medical literature. 2) Possibly, this mutation is linked to premature baldness. PMID: 28741586
    15. CASR SNPs may partly explain differences in the clinical manifestations of CKD-MBD between european and African ancestry populations, and in the biochemical response to cinacalcet in many patients. PMID: 28630081
    16. Decreased sensitivity of the CaSR to calcium due to inactivating polymorphisms at rs1801725, may predispose up to 20% of breast cancer cases to high circulating calcium-associated larger and/or aggressive breast tumors. PMID: 28764683
    17. This study shows that the A allele of rs7652589 is a risk allele for nephrolithiasis-related end stage renal disease. The AA genotype is associated with more severe secondary hyperparathyroidism (higher calcium and parathormone concentrations). PMID: 27739473
    18. Polymorphism of the Calcium-Sensing Receptor Gene is associated with Breast Cancer Risk.[review] PMID: 29504802
    19. Data show that Cao2+ via CaR- mediated signaling induces filamin A cleavage and promotes the migration in AR-deficient and highly metastatic prostate cancer cells. PMID: 27206800
    20. GPR64 is expressed on the cell surface of parathyroid cells, is overexpressed in parathyroid tumors, and physically interacts with the CaSR. PMID: 27760455
    21. this study, demonstrates for the first time that calcium exerts an oncogenic action in the stomach through activation of CaSR and TRPV4 channels. Both CaSR and TRPV4 were involved in Ca2+-induced proliferation, migration, and invasion of gastric cancer cells through a Ca2+/AKT/beta-catenin relay, which occurred only in gastric cancer cells or normal cells overexpressing CaSR. PMID: 28951460
    22. mutagenesis with a novel analytical approach and molecular modeling to develop an "enriched" picture of structure-function requirements for interaction between Ca(2+)o and allosteric modulators within the CaSR's 7 transmembrane (7TM) domain, is reported. PMID: 27002221
    23. FLNA is downregulated in parathyroid tumors and parallels the CASR expression levels. Loss of FLNA reduces CASR mRNA and protein expression levels and the CASR-induced ERK phosphorylation. FLNA is involved in receptor expression, membrane localization and ERK signaling activation of both 990R and 990G CASR variants. PMID: 27872158
    24. A father and daughter with asymptomatic chronic hypocalcemia with low parathyroid hormone and inappropriate urinary calcium excretion had a missense mutation in exon 7: c.2621G>T (p.Cys874Phe). PMID: 27663953
    25. These results support the emerging potential of CaSR as a therapeutic target in metastatic breast cancer whose pharmacological modulation would reduce IL-6. PMID: 27477783
    26. These structures reveal multiple binding sites for Ca(2+) and PO4(3-) ions. Both ions are crucial for structural integrity of the receptor. While Ca(2+) ions stabilize the active state, PO4(3-) ions reinforce the inactive conformation. PMID: 27434672
    27. The endoplasmic reticulum-associated protein, OS-9, behaves as a lectin in targeting the immature calcium-sensing receptor. PMID: 28419469
    28. Glucose acts as a positive allosteric modulator of CaSR. PMID: 27613866
    29. These studies indicate that the CaSR activation impairs glucose tolerance by a combination of alpha- and beta-cell defects and also influences pancreatic islet mass. PMID: 28575322
    30. Minor alleles rs7652589 and rs1501899 are associated with reduced CaSR expression in neuroblastic tumors and neuroblastoma cell lines in which the CASR gene promoter P2 is not hypermethylated. PMID: 27862333
    31. Calcium exerted its effects on cartilaginous endplates matrix protein synthesis through activation of the extracellular calcium-sensing receptor. PMID: 27452962
    32. Polymorphic variations in VDR and CASR may be associated with survival after a diagnosis of colorectal neoplasms. PMID: 28765616
    33. CaSR and AP2S1 sequencing is worthwhile in patients with familial hyperparathyroidism and phenotype suggesting familial hypocalciuric hypercalcemia as it can diagnose up to 50% of cases. PMID: 28176280
    34. reduced expression of the CaSR is correlated with activation of the renin-angiotensin system, which induces increased vascular remodeling and vascular smooth muscle cell proliferation, and thereby associated with essential hypertension in the SHR rat model and in the Han Chinese population PMID: 27391973
    35. CaSR exerts a suppressive function in pancreatic tumorigenesis through a novel NCX1/Ca(2+)/beta-catenin signaling pathway. PMID: 27108064
    36. In whites, CaSR gene SNP rs1801725 was associated with serum calcium but not with risk of diabetes. PMID: 27510541
    37. Tumor CaSR expression is associated with an increased risk of lethal prostate cancer, particularly in tumors with low VDR expression. PMID: 27115058
    38. Functional interaction of upregulated CaSR and upregulated TRPC6 in pulmonary artery smooth muscle cells from idiopathic pulmonary arterial hypertension patients may play an important role in the development and progression of sustained pulmonary vasoconstriction and pulmonary vascular remodeling. PMID: 26968768
    39. This is a prospective observational study that measures the expression of vitamin D (VD) metabolising and signalling molecules and Ca(2+) sensing receptor (CaSR) in human Fallopian tube (FT) during the menstrual cycle and ectopic pregnancy (EP). PMID: 27770255
    40. CaSR expression was shown in HepG2 cells and human liver samples; CaSR may contribute to obesity-associated hepatic metabolic consequences PMID: 27565442
    41. Polymorphisms of CASR gene increase the risk of primary hyperparathyroidism. PMID: 26710757
    42. Calcium oxalate-induced renal injury is related to CaSR-mediated oxidative stress and increased mitogen-activated protein kinase signaling, which subsequently leads to CaOx crystal adhesion. PMID: 27965733
    43. The detection of CaSR gene mutations is suitable to differentiate states of hypercalcemia and may help to avoid invasive procedures such as parathyroidectomies. PMID: 27926951
    44. a novel loss-of-function mutation, G571W, in the CaSR gene in a Korean family with familial hypocalciuric hypercalcemia. PMID: 26386835
    45. There is a significant correlation between in vitro functional impairment of the CaSR at physiologic calcium concentrations and the severity of alterations in calcium homeostasis in patients. PMID: 27666534
    46. Calcium sensing receptor gene rs1801725 variants are not associated with susceptibility to colorectal cancer. PMID: 25124570
    47. physiological fetal hypercalcemia, acting on the CaSR, promotes human fetal lung development via cAMP-dependent opening of CFTR. PMID: 26911344
    48. CaSR and PTH1R signaling responses in cartilage and bone. [review] PMID: 26688334
    49. The calcium-sensing receptor may be involved in the modulation of inflammatory processes. [review] PMID: 26303192
    50. A986S polymorphism of CaSR is an independent predictor of PTH level in normocalcemic hyperparathyroidism patients, but not in asymptomatic hyperparathyroidism. PMID: 26332755

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  • 相關(guān)疾病：
    Hypocalciuric hypercalcemia, familial 1 (HHC1); Hyperparathyroidism, neonatal severe (NSHPT); Hypocalcemia, autosomal dominant 1 (HYPOC1); Epilepsy, idiopathic generalized 8 (EIG8)
  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 3 family
  • 組織特異性：
    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1514

    OMIM: 145980

    KEGG: hsa:846

    STRING: 9606.ENSP00000420194

    UniGene: Hs.435615