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  4. Recombinant Human Fanconi anemia group I protein (FANCI), partial

Recombinant Human Fanconi anemia group I protein (FANCI), partial

  • 中文名稱：
    人FANCI重組蛋白
  • 貨號：
    CSB-YP889120HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人FANCI重組蛋白
  • 貨號：
    CSB-EP889120HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人FANCI重組蛋白
  • 貨號：
    CSB-EP889120HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人FANCI重組蛋白
  • 貨號：
    CSB-BP889120HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人FANCI重組蛋白
  • 貨號：
    CSB-MP889120HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    FANCI
  • Uniprot No.：
  • 別名：
    FANCI; FANCI gene; FANCI_HUMAN; Fanconi anemia group I protein; Fanconi anemia; complementation group I; FLJ10719; FLJ14658; KIAA1794; Protein FACI; Protein FANCI
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.
  • 基因功能參考文獻：
    1. BRMS1FANCI interaction is necessary for the regulatory role of BRMS1 in the FA pathway. PMID: 30365131
    2. Study reports the first structural insight into the human FANCD2-FANCI complex by obtaining the cryo-EM structure. The complex contains an inner cavity, large enough to accommodate a double-stranded DNA helix, as well as a protruding Tower domain. Disease-causing mutations in the Tower domain are observed in several Fanconi anemia patients. PMID: 27405460
    3. FANCI phosphorylation activates the FANCI/D2 complex. PMID: 28636932
    4. Data suggest that FANCI and FANCD2 have partially non-overlapping and possibly even opposing roles during the replication stress response. PMID: 29059323
    5. Fanconi anemia FANCD2 and FANCI proteins regulate the nuclear dynamics of splicing factors, such as SF3B1. PMID: 29030393
    6. FANCB dimer coordinates FANCD2:FANCI monoubiquitination by two FANCL RING-ligases. Deubiquitination of FANCD2:FANCI by USP1:UAF1 occurs only when DNA is removed. PMID: 27986371
    7. depletion of FANCI, but not FANCD2 or USP1, results in increased phosphorylation and activation of Akt. PMID: 27097374
    8. FANCI mutations are associated with Fanconi anemia in VACTERL association. PMID: 26590883
    9. FANCJ protein is important for the stability of FANCD2/FANCI proteins and protects them from proteasome and caspase-3 dependent degradation. PMID: 26336824
    10. These findings indicate that FANCI functions upstream of FA core complex recruitment independently of FANCD2, and alter the current view of the FA-BRCA pathway. PMID: 26430909
    11. these purification methods for human FANCI and FANCD2 provide novel procedures to facilitate structural and biochemical studies of human FANCI and FANCD2. PMID: 25168188
    12. Results show that ATR-mediated phosphorylation of FANCI, controls dormant origin firing in response to DNA replication stress. PMID: 25843623
    13. Our studies reveal a previously unknown mechanism for the coordinate nuclear import of a subset of FANCD2 and FANCI, a key early step in the cellular ICL response. PMID: 24278431
    14. Mutations in FANCI that impair its DNA binding activity compromise DNA-stimulated FANCD2 monoubiquitination. PMID: 24623813
    15. A CUE ubiquitin-binding domain in the FANCD2 protein is required for interaction with FANCI, retention of monoubiquitinated FANCD2, and FANCI in chromatin, and for efficient DNA interstrand crosslinks repair. PMID: 22855611
    16. These data suggest a key role for the E3 ligase activity of RAD18 in the recruitment of FANCD2 and FANCI to chromatin and the events leading to their ubiquitylation during S phase. PMID: 21355096
    17. although proper nuclear localization of FANCI is crucial for robust FANCD2 monoubiquitination, the putative FANCI EDGE motif is important for DNA crosslink repair PMID: 20971953
    18. study characterizes FANI which promotes DNA interstrand cross-linking repair in a manner strictly dependent on its ability to accumulate at or near sites of DNA damage and that relies on mono-ubiquitylation of the FANCI-FANCD2 complex PMID: 20671156
    19. description of the Fanconi anemia protein FANCI, identified as an ATM/ATR kinase substrate required for resistance to mitomycin C; it associates with FANCD2 and, together, as the FANCI-FANCD2 (ID) complex, localizes to chromatin in response to DNA damage PMID: 17412408
    20. These data add up to two conclusions. First, KIAA1794 is a Fanconi anemia gene. Second, this gene is identical to FANCI, since the patient cell lines found mutated in this study included the reference cell line for group I, EUFA592. PMID: 17452773
    21. FANCI, a member of the Fanconi anemia pathway, is monoubiquitinated in a site-specific and DNA damage dependent manner. PMID: 17460694
    22. Results suggest that the multiple phosphorylation of FANCI serves as a molecular switch in activation of the Fanconi anemia pathway. PMID: 18931676
    23. This work therefore establishes a system that provides mechanistic insight into the functions of FANCL and FANCI in the catalysis of FANCD2 monoubiquitination. PMID: 19111657
    24. Data show that FANCD2 and FANCI specifically associate with common fragile site loci irrespective of whether the chromosome is broken, and that these loci are frequently interlinked through BLM-associated ultra-fine DNA bridges through mitosis. PMID: 19465922
    25. the FANCI-FANCD2 complex may participate in repair of damaged replication forks through its preferential recognition of branched structures. PMID: 19561358
    26. results rule out a major role of FANCI in familial breast cancer susceptibility PMID: 19737859

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  • 相關(guān)疾病：
    Fanconi anemia complementation group I (FANCI)
  • 亞細胞定位：
    Nucleus. Cytoplasm. Note=Observed in spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites. They are frequently interlinked through BLM-associated ultra-fine DNA bridges.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 25568

    OMIM: 609053

    KEGG: hsa:55215

    STRING: 9606.ENSP00000310842

    UniGene: Hs.513126