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  4. Recombinant Human Glutamate receptor 2 (GRIA2), partial

Recombinant Human Glutamate receptor 2 (GRIA2), partial

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  • 中文名稱：
    人GRIA2重組蛋白
  • 貨號：
    CSB-EP009899HU
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    (GluR-2)(AMPA-selective glutamate receptor 2)(GluR-B)(GluR-K2)(Glutamate receptor ionotropic, AMPA 2)(GluA2)
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    63.3 kDa
  • 表達區(qū)域：
    25-543aa
  • 氨基酸序列
    NSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDEMYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTTTIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system. It plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting.
  • 基因功能參考文獻：
    1. This review showed that the mutation GR1A protein associate with Obsessive-compulsive disorder. PMID: 28608743
    2. Measured the expression of GRIA2 and GABRA1 in patients with methamphetamine-use disorder. Also examined whether miR-181a down-regulates GRIA2 and GABRA1 in a cell-based assay. We further examined the effects of chronic methamphetamine exposure on the expression of miR-181a, GRIA2 and GABRA1. The results demonstrated that serum GRIA2 is higher in patients with methamphetamine-use disorder than in healthy controls. PMID: 27767084
    3. This study demonstrated that a significant decrease in the protein level of GluN2A in major depression disorder. PMID: 27661418
    4. both the intracellular C-terminal domain (CTD) and the loop region between the M1 and M2 helices move during activation and the CTD is detached from the membrane PMID: 27313205
    5. The results of this study suggest that neurons in hypothalamic hamartoma may bear Ca(2+) -permeable AMPA receptors(GluA2 ) due to dislocation of ADAR2 PMID: 28195308
    6. A transient positive feedback mechanism between AMPAR and stargazin has implications for information processing in the brain, because it should allow activity-dependent facilitation of excitatory synaptic transmission through a postsynaptic mechanism. PMID: 26744192
    7. The GluR2 subunit of the AMPA receptor is involved in cell migration and calcium signaling. PMID: 26311781
    8. RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition PMID: 25784538
    9. GRIA2*CCC polymorphism is genetic risk marker for paranoid schizophrenia in Russians.Low risk genetic markers of paranoid schizophrenia were revealed: in Tatars-GRIA2*T/T (rs43025506) of GRIA2 gene and GRIA2*CCT in Russians. PMID: 25842862
    10. GRIA2 is a useful marker for distinguishing solitary fibrous tumour from most mimics PMID: 24456377
    11. a link between neurodegenerative processes and deficient RNA editing of the GluA2 Q/R site. PMID: 24679603
    12. Statistical analysis showed no association between migraine and the GRIA2 and GRIA4 polymorphisms investigated. PMID: 24512576
    13. analysis of changes in receptor kinetics with the R628E charge-inverting mutation in the "linker" region of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor PMID: 24550387
    14. We show that excitatory cortical-patterned neurons derived from multiple human pluripotent stem cell lines exhibit native-like maturation changes in AMPAR composition such that there is an increase in the expression of GluA2(R) subunits. PMID: 24623784
    15. The data potentially suggest a lack of epistatic interaction between GRIA2 and GRIA4 variants regarding clinical outcomes in patients with major depressive disorder. PMID: 23613500
    16. The ionotrophic glutamate receptors AMPA2 and AMPA3 were decreased in hippocampus in patient with multiple sclerosis. PMID: 23595422
    17. ADAR2 expression level reflects editing activity at the GluA2 Q/R site; although the edited GluA2 pre-mRNA is readily spliced, the unedited GluA2 pre-mRNA is also spliced and transported to the cytoplasm when ADAR2 expression is low. PMID: 22366356
    18. Did not observed significant association between GRIA2 polymorphisms and clinical improvement in patients with Major depressive disorder.But rs4302506 and rs4403097 single nucleotide polymorphisms could be associated with age of onset of the disease. PMID: 22057216
    19. We demonstrated that the expression of GRIA2 among the differentially expressed genes provides better prognosis of patients with advanced serous papillary ovarian adenocarcinoma. PMID: 22644307
    20. The balance of expression of nerve growth factor (NGF) and that of its receptors shifts toward cell death mechanisms during the progression of Alzheimer disease. PMID: 21397006
    21. the GluR2 gene is embedded into an open chromatin configuration in glioma cells and expression of GluR2 is controlled by REST and Sp1 PMID: 21948504
    22. SNPs within GRIA2 may not be associated with the development and treatment outcomes in BD PMID: 22122651
    23. significant association GRIA2 polymorphisms was found with the diagnosis of schizophrenia. PMID: 22094384
    24. We conclude that polymorphisms in the GluR2 gene (GRIA2) are not a major contributory factor in the pathogenesis of ALS. PMID: 20409611
    25. Data show that CALM influences the cell surface level of the AMPA receptor subunit GluR2. PMID: 21221849
    26. Gain-of-function glutamate receptor interacting protein 1 variants alter GluA2 recycling and surface distribution in patients with autism PMID: 21383172
    27. Transsynaptic signaling mediated by the extracellular domain of GluR2 regulates the stability of presynaptic terminals. PMID: 21173224
    28. [review] The importance of mGlu2/3 glutamate receptors during normal central nervous system development is likely to play an important mechanistic role in the neurodevelopmental hypothesis of schizophrenia. PMID: 19933774
    29. Post-transcriptional editing of this subunit at the Q/R/N site controls calcium permeability PMID: 11860506
    30. role of interaction with N-ethylmaleimide-sensitive factor in regulation of AMPA receptors by brain-derived neurotrophic factor PMID: 12130635
    31. Low levels of GluR2 mRNA in motoneurons of ALS did not differ from the control group, implying that selective reduction of the GluR2 subunit cannot be a mechanism of AMPA receptor-mediated neurotoxicity in ALS PMID: 12694394
    32. the Q/R site of GluRs editing is regulated in a regional manner and the GluR2 Q/R site editing is critically regulated by ADAR2 in human brain PMID: 12859334
    33. GluR2, a subunit of a ligand-gated cation channel, is up-regulated in leiomyomata relative to myometrium by 15- to 30-fold at the protein and mRNA level and is localized in endothelial cells PMID: 14630051
    34. There is a defect in the editing of the mRNA encoding the GluR2 subunit of glutamate AMPA receptors in the spinal motor neurons of individuals affected by amyotrophic lateral sclerosis PMID: 14985749
    35. R/G editing status: the relative amount of edited GLUR2 mRNA was increased in epileptic hippocampi, whereas no changes were found in neocortical tissues. PMID: 15006707
    36. Control of GLUR2 translation was mediated by a sequence containing a 34-42 nucleotide imperfect GU repeat predicted to form secondary structure in vivo. This translation suppression domain is included in some rat and human GluR2 transcripts in vivo PMID: 15071096
    37. GluR2 was localized to the perikarya and proximal dendrites of BLA neurons; dense labeling was also present over the pyramidal cell layer of hippocampal subfields CA1 and CA3 PMID: 16045445
    38. Circumstance data have indicated that the GluR2 subunits dictate Ca2+/Zn2+ permeability of AMPA receptor channels and gate injurious Ca2+/Zn2+ signals in vulnerable neurons. PMID: 16215279
    39. Laser capture microdissection combined with quantitative PCR was used to examine the expression of AMPA (GRIA1-4) and NMDA (GRIN1, 2A and 2B) subunit mRNA levels in Layer II/III and Layer V pyramidal cells PMID: 17942280
    40. Of the three AMPA genes analyzed here, only GRIA3 seems to be involved in the pathogenesis of schizophrenia, but only in females. PMID: 18163426
    41. utilized reverse-transcription polymerase chain reaction and BbvI digestion to demonstrate that neural progenitor cells contain Q/R-unedited GluR2, and differentiated cells contain Q/R-edited GluR2 subunits PMID: 18403631
    42. the GluR4 subunit exhibits a different correlation between receptor activation and ligand-binding domain cleft closure than does GluR2 PMID: 19102704
    43. These findings indicate that the absence of the GluR2 subunit favours malignancy in gliomas PMID: 19558602
    44. This dimeric structure provides a mechanism for how the ATDs can drive receptor assembly and subtype-restricted composition. PMID: 19651138

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic density membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Glutamate-gated ion channel (TC 1.A.10.1) family, GRIA2 subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4572

    OMIM: 138247

    KEGG: hsa:2891

    STRING: 9606.ENSP00000264426

    UniGene: Hs.32763