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  4. Recombinant Human Glutamate receptor ionotropic, NMDA 2A (GRIN2A), partial

Recombinant Human Glutamate receptor ionotropic, NMDA 2A (GRIN2A), partial

  • 中文名稱(chēng)：
    人GRIN2A重組蛋白
  • 貨號(hào)：
    CSB-EP618634HU1
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    EPND; FESD; GluN2A; Glutamate [NMDA] receptor subunit epsilon-1; Glutamate receptor; Glutamate receptor ionotropic N methyl D aspartate 2A; GRIN 2A; GRIN2A; hNR2A; LKS; N methyl D aspartate receptor channel; subunit epsilon 1; N Methyl D Aspartate Receptor Subtype 2A; N methyl D aspartate receptor subunit 2A; N-methyl D-aspartate receptor subtype 2A; NMDA receptor subtype 2A; NMDAR 2A; NMDAR2A; NMDE1_HUMAN; NR2A; OTTHUMP00000160135; OTTHUMP00000174531
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Partial
  • 來(lái)源：
    E.coli
  • 分子量：
    18.2kDa
  • 表達(dá)區(qū)域：
    501-550aa & 601-630aa & 701-750aa
  • 氨基酸序列
    VYQRAVMAVGSLTINEERSEVVDFSVPFVETGISVMVSRSNGTVSPSAFLIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMMHQYMTKFNQKGVEDALVSLKTGKLDAFIYDAAVLNYKAGRDEGCKLVTI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer,50% glycerol
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B. Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning.
  • 基因功能參考文獻(xiàn)：
    1. GRIN2A rare deleterious and loss of function variants are specifically associated with typical and atypical Rolandic epilepsy. PMID: 29358611
    2. GRIN2A mutation is associated with epilepsy-aphasia spectrum disorders but with a relatively low rate. PMID: 29896722
    3. Lifetime mood disorder emerged as a more significant factor for suicidal ideation in systemic lupus erythematosus compared with NR2A gene polymorphism main and interaction effects. PMID: 29161964
    4. The results of this study GRIN2A mutation is a genetic cause in less than 11% patients with Landau-Kleffner syndrome or atypical benign partial epilepsy. PMID: 29056244
    5. Mutations P79R, C231Y, G483R and M705V caused a significant reduction in glutamate and glycine agonist potency, whilst D731N was non-responsive. These mutants, along with E714K, also showed significantly decreased total protein levels and trafficking to the cell surface, whilst C436R was not trafficked at all. PMID: 28242877
    6. This review showed that GRIN2A associate with Obsessive-compulsive disorder. PMID: 28608743
    7. This study demonstrates that GRIN2A mutations in melanoma cause heterogenous effects but some may be oncogenic. PMID: 27659111
    8. data indicate that children with epilepsy due to pathogenic GRIN2A mutations present with different clinical phenotypes and a spectrum of seizure types in the context of a pharmacoresistant epilepsy. PMID: 28109652
    9. a de novo missense mutation in the GRIN2A gene in a patient with childhood focal epilepsy and acquired epileptic aphasia. The mutant decreases NMDAR activation suggesting NMDAR hypofunction may contribute to the epilepsy pathogenesis. PMID: 28182669
    10. genetic association studies in population in China: Data suggest that one SNP in GRIN2A (rs2650429; but not rs6497540 or rs9302415) is associated with lead-induced neurotoxicity; in this case-control study, cases were lead-exposed workers from battery plants. Lead-exposed individuals have lower serum GRIN2A levels compared with controls; lead decreases GRIN2A expression levels in HEK293 cell. PMID: 27230353
    11. Two adjacent phenylalanines in the NMDA receptor GluN2A subunit M3 domain interactively regulate alcohol sensitivity and ion channel gating. PMID: 27876530
    12. The GRIN2A genotype was not associated with the rate of clinical progression of PD in the placebo group. PMID: 28320167
    13. most rare variants in GluN2A were associated with epilepsy, whereas GluN2B variants were associated with intellectual disability with or without seizures PMID: 27839871
    14. the gain-of-function M817V mutation causes overactivation of NMDAR and drives neuronal hyperexcitability. PMID: 28126851
    15. To determine genetic variability within the N-methyl-D-aspartate receptor 2A sub-unit (GRIN2A) gene promoter and its association with concussion recovery time. PMID: 26502998
    16. Findings suggest that mutations in GRIN2A preferentially are involved in genetic variance of pediatric idiopathic generalized epilepsy and do not contribute significantly to either adult focal epilepsies as temporal lobe epilepsy or generalized epilepsies PMID: 26220384
    17. Based on large, well-characterized datasets independent from the original study, our results are not in favor of an interaction between caffeinated coffee consumption and GRIN2A rs4998386 for Parkinson disease risk PMID: 25412286
    18. NMDARs have a dual role during erythropoiesis, supporting survival of polychromatic erythroblasts and contributing to the Ca(2+) homeostasis from the orthochromatic erythroblast stage to circulating red blood cells. PMID: 25788577
    19. GRIN2A (GT)21 may play a significant role in the etiology of schizophrenia among the Chinese Han population of Shaanxi. PMID: 25958346
    20. GRIN2A gene polymorphisms confer susceptibility to heroin addiction. PMID: 25366762
    21. Dysarthria and dyspraxia are found in individuals with GRIN2A mutations, often in the setting of epilepsy-aphasia syndromes. The speech phenotype may occur in the absence of a seizure disorder, reinforcing the role for GRIN2A in motor speech function. PMID: 25596506
    22. The reported association of a single nucleotide polymorphism, GRIN2A_rs4998386, and its interaction with caffeine intake with PD in Sweden, was replicated. PMID: 24915238
    23. We demonstrate the functional importance of GRIN2A mutations in melanoma and the significance that ionotropic glutamate receptor signaling has in malignant melanoma. PMID: 24739903
    24. study describes two children with a GRIN2A mutation and epilepsy, with one having a microdeletion on chromosome 16p13 PMID: 24125812
    25. Array comparative genomic hybridization (aCGH) is used to confirm genes for epileptic and other neurodevelopmental disorders, including GRIN2A and proline-rich transmembrane protein (PRRT), both found in rolandic epilepsy. PMID: 24372385
    26. A possible role for GRIN2A polymorphisms in heroin addiction. PMID: 23940648
    27. The sigma-1 receptor was coisolated with GluN1 receptor subunit but not with the GluN2A subunit. PMID: 24227730
    28. GRIN2A mutations cause epilepsy-aphasia spectrum disorders. PMID: 23933818
    29. Mutations in GRIN2A cause idiopathic focal epilepsy with rolandic spikes. PMID: 23933819
    30. GluN2A(F636) plays an important role in both channel function and ethanol inhibition in NMDA receptors. PMID: 23847085
    31. The results of this study indicated that rs2072450 of GRIN2A might confer risk for alcohol dependence through deficient fear acquisition indexed by a diminished amygdala response during aversive learning,. PMID: 23693003
    32. GRIN2A single nucleotide polymorphisms are asociated with phenotypic brain volume variations in multiple slcerosis. PMID: 22851457
    33. After 7 days of chronic alcohol exposure there are significant increases in mRNA expression of GRIN2A in cultured neurons derived from alcoholic subjects, but not in cultures from nonalcoholics. PMID: 22486492
    34. Two de novo mutations in GRIN2A are identified in patients with sporadic schizophrenia. PMID: 22833210
    35. The results show a significant association between polymorphism of the GRIN2A gene and alcoholism. PMID: 21507155
    36. NMDA subunit NR2A is not involved in amygdala hyperexcitability of patients with temporal lobe epilepsy. PMID: 20848605
    37. glutamate receptor gene GRIN2A is a Parkinson's disease modifier gene via interaction with coffee PMID: 21876681
    38. Polymorphism rs11866328 in the GRIN2A gene might be a genetic variant underlying the susceptibility of HBV carriers to disease progression. PMID: 22004137
    39. identified as prime candidate gene for photoparoxysmal response PMID: 21883175
    40. This review focuses on the role of the NMDA receptor subunits in schizophrenia, particularly via altering the balance of NR2 subunits during early development, could influence NRG1 signalling {REVIEW} PMID: 21371516
    41. Exome sequencing identifies GRIN2A as frequently mutated in melanoma. PMID: 21499247
    42. Data show that that all seven currently known NMDAR subunits (NR1, NR2A, NR2B, NR2C, NR2D, NR3A and NR3B) are expressed in astrocytes, but at different levels. PMID: 21152063
    43. In melanoma, GRIN2A is identified as a common somatic mutation. PMID: 21499247
    44. Mutations cause mental neurodevvelopmental disordes such as epilepsy and mental retardation. PMID: 20890276
    45. Data demonstrate that tat treatment of human neurons results in tyrosine phosphorylation of the NMDAR subunit 2A (NR2A) in a src kinase-dependent manner. PMID: 20448061
    46. Functional (GT)n polymorphisms in promoter region of N-methyl-d-aspartate receptor 2A subunit (GRIN2A) gene affect hippocampal and amygdala volumes PMID: 20070378
    47. the PFC, glutamatergic regulation of PV-containing inhibitory neurons via NR2A-containing NMDA receptors does not appear to be altered in bipolar disorder. PMID: 20148871
    48. Tyrosine-1325 phosphorylation regulates N-methyl-D-aspartate (NMDA) receptor channel properties and NMDA receptor-mediated downstream signaling to modulate depression-related behavior. PMID: 19834457
    49. The C-terminus of NR2A is critical for modulation of desensitization by calcineurin. PMID: 11985816
    50. The levels of NR2A mRNA are decreased in Alzheimer disease hippocampus and entorhinal cortex compared to controls. PMID: 12127670

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  • 相關(guān)疾病：
    Epilepsy, focal, with speech disorder and with or without mental retardation (FESD)
  • 亞細(xì)胞定位：
    Cell projection, dendritic spine. Cell membrane; Multi-pass membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Glutamate-gated ion channel (TC 1.A.10.1) family, NR2A/GRIN2A subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 4585

    OMIM: 138253

    KEGG: hsa:2903

    STRING: 9606.ENSP00000332549

    UniGene: Hs.411472