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  4. Recombinant Human Guanine nucleotide-binding protein-like 3 (GNL3)

Recombinant Human Guanine nucleotide-binding protein-like 3 (GNL3)

  • 中文名稱：
    人GNL3重組蛋白
  • 貨號(hào)：
    CSB-YP874819HU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    人GNL3重組蛋白
  • 貨號(hào)：
    CSB-EP874819HU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    人GNL3重組蛋白
  • 貨號(hào)：
    CSB-EP874819HU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人GNL3重組蛋白
  • 貨號(hào)：
    CSB-BP874819HU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人GNL3重組蛋白
  • 貨號(hào)：
    CSB-MP874819HU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GNL3
  • Uniprot No.：
  • 別名：
    C77032 ; E2 induced gene 3 protein; E2-induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; GNL3_HUMAN; Guanine nucleotide binding protein like 3 (nucleolar); Guanine nucleotide binding protein like 3; Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3 ; Nucleolar GTP-binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-549
  • 氨基酸序列
    MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKKR KLETNPDIKP SNVEPMEKEF GLCKTENKAK SGKQNSKKLY CQELKKVIEA SDVVLEVLDA RDPLGCRCPQ VEEAIVQSGQ KKLVLILNKS DLVPKENLES WLNYLKKELP TVVFRASTKP KDKGKITKRV KAKKNAAPFR SEVCFGKEGL WKLLGGFQET CSKAIRVGVI GFPNVGKSSI INSLKQEQMC NVGVSMGLTR SMQVVPLDKQ ITIIDSPSFI VSPLNSSSAL ALRSPASIEV VKPMEAASAI LSQADARQVV LKYTVPGYRN SLEFFTVLAQ RRGMHQKGGI PNVEGAAKLL WSEWTGASLA YYCHPPTSWT PPPYFNESIV VDMKSGFNLE ELEKNNAQSI RAIKGPHLAN SILFQSSGLT NGIIEEKDIH EELPKRKERK QEEREDDKDS DQETVDEEVD ENSSGMFAAE ETGEALSEET TAGEQSTRSF ILDKIIEEDD AYDFSTDYV
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.
  • 基因功能參考文獻(xiàn)：
    1. GNL3 promotes the EMT in colon cancer by activating the Wnt/betacatenin signaling pathway. GNL3 is upregulated in colon cancer and plays an important role in tumor growth, invasion and metastasis. PMID: 28849076
    2. In addition, migration and invasion demonstrated significant inhibition in Nucleostemin-deficient cells. Furthermore, the knockdown of Nucleostemin dramatically suppressed xenograft progression in BALB/c nude mice. Our findings suggest that Nucleostemin is associated with malignancy in an ovarian cancer SKOV-3 cell line. PMID: 28412352
    3. Identified a positive correlation between up-regulation of nucleostemin and human glioma cell proliferation and that knocking-down nucleostemin alleviated glioma proliferation by reducing beta-Catenin transportation into the nucleus. PMID: 26607678
    4. Relative expression of GNL3 in synovial tissue and fluid samples was significantly higher in the osteoarthritic group as compared to the non-osteoarthritic group. PMID: 27066995
    5. The expression level of GNL3 was examined in 89 human gastric cancer samples using immunohistochemistry (IHC) staining. GNL3 in gastric cancer tissues was significantly upregulated compared with paracancerous tissues. PMID: 26722529
    6. Study demonstrated overexpression of NS in most hepatocellular carcinoma tissues compared with their matched surrounding tissues, and silencing NS promoted UV- and serum starvation-induced apoptosis of MHCC97H and Bel7402 cells. PMID: 26517370
    7. over-expression of GNL3 and SKA3 in the PC-3 human prostate cancer cell line decreased in vitro cell migration and invasion PMID: 26429724
    8. overexpression of NS suppressed ARF polyubiquitination by its E3 ligase Ubiquitin Ligase for ARF and elongated its half-life, whereas knockdown of NS led to the decrease of ARF levels PMID: 24769896
    9. Silence of nucleostemin inhibit cell proliferation through up-regulating p21 expression and nucleostemin may be involved in the pathogenesis of esophageal squamous cell carcinoma. PMID: 25699361
    10. It is concluded that nucleostemin expression could not be specific in a certain type of cells in colon cancer cell line HT29. PMID: 24762489
    11. Data shows that the NS protein expression status was positively correlated with both ER and HER2 status and was a powerful prognostic factor. PMID: 24650343
    12. Based on our results, NS overexpression seems to have a causative role in gastric tumorigenesis and/or progression, and it could be considered as a potential tumor marker for diagnosis, molecular classification PMID: 24763828
    13. Gene expression profiling analysis revealed that cell cycle regulators and nucleotide metabolism-related genes were highly enriched in a gene set associated with leukaemia-initiating capacity that we termed the 'leukaemia stem cell gene signature'. PMID: 24960197
    14. cis-acting regulatory polymorphisms acting on GNL3 and SPCS1 contribute to the OA association signal at chromosome 3p21, and these genes therefore merit further investigation. PMID: 24886551
    15. Nucleostemin can be used for tracking minimal residual disease and is a helpful guide for treatment. PMID: 24119536
    16. NS functions downstream of Myc as a rate-limiting regulator of cell proliferation and transformation. PMID: 22081066
    17. The nucleostemin(NS)could promote the recruitment of PML-IV to SUMOylated TRF1 in TA( ) and ALT cells. PMID: 22641345
    18. NS undergoes a ubiquitin- and MDM2-independent proteasomal degradation when intracellular GTP levels are markedly reduced PMID: 22318725
    19. Nucleostemin is expressed in Glioblastoma-cancer stem cells isolated from patient samples, and that its expression, conversely to what it has been described for ordinary stem cells, does not disappear when cells are differentiated. PMID: 22174890
    20. nucleostemin expression level correlated with TWIST expression level in esophageal cancer cell lines; identified that high nucleostemin proportion, TWIST intensity, and advanced pathological N stage were correlated with poor relapse-free survival PMID: 22050045
    21. NS inhibits TRF1 dimerization and shortens TRF1 association with the telomere. PMID: 22045740
    22. a complex composed of TERT, BRG1, and NS/GNL3L maintains the function of tumor initiating cells PMID: 21730156
    23. expression in ovarian tumors closely correlated with origination, progression, and grading PMID: 21892090
    24. NS is widely expressed in normal and neoplastic oral epithelial tissues, and is likely a marker of proliferation PMID: 21443540
    25. Apoptosis of HL-60 leukemia cells can be induced by silencing NS gene expression. PMID: 19379559
    26. Reactive oxygen species regulate nucleostemin oligomerization and protein degradation. PMID: 21242306
    27. Data show that knockdown of NS dramatically reduced the sphere-forming activity of MCF7 and MDA-MB-231 breast cancer cells. PMID: 21045149
    28. NS may promote the progression of prostate cancer by inhibiting the expression of p15, p16, and p18 in PC-3 cells. PMID: 20021940
    29. Nucleostemin is an important G1/S checkpoint regulator and it could regulate cell cycles via a p53-independent pathway in prostate cancer. PMID: 20664182
    30. Differential expression of NS, a stem cell marker, and its variants in different types of brain tumor are reported. PMID: 20572164
    31. The upregulation of nucleostemin ,mRNA frequently occurs in ESCC tissues and is associated with malignancy of human esophageal squamous tumors. nucleostemin is required for EGF and EGFR expression. PMID: 19823871
    32. Nucleostemin mRNA and protein are over-expressed in human esophageal squamous cell carcinoma. PMID: 18646696
    33. Data suggest that nucleostemin can help regulate the proliferation of both cancer cells and stem cells, and may play an important role in the growth regulation of gastric cancer, liver cancer and other cancers. PMID: 15112336
    34. nucleostemin has a role in proliferation and tumorigenesis of HeLa cells PMID: 15595646
    35. The nucleolar residence of nucleostemin involves a transient and a long-term binding by the basic and GTP-binding domains, and a dissociation mechanism mediated by the COOH-terminal region. PMID: 15657390
    36. analysis of nucleolar localization of stem cell protein nucleostemin PMID: 15857956
    37. Results implied that nucleostemin may play an important role in both tumorigenesis and transforming human embryonic bone marrow mesenchymal stem cells into F6 tumor cells. PMID: 16012751
    38. Transfected protein interacts with several proteins in COS cells. PMID: 16097049
    39. Using a multipotential adherent population of stem cells, nucleostemin was localized to the nucleoli and occurred in 43.3% of the cells. PMID: 16282439
    40. Expression widely occurs in normal and malignant renal tissues, and is likely a proliferation marker rather than a unique regulator of cell proliferation and survival in stem and cancer cells. PMID: 16670719
    41. In cultured cells, nucleostemin is expressed in a distinct population of epidermal cells from hair follicle kept in the presence of a feeder layer, intimating an association of nucleostemin expression with this type of epithelio-mesenchymal interaction PMID: 17000083
    42. Transgenic overexpression of nucleostemin increases the population doubling and decreases the senescence of mouse embryonic fibroblasts. PMID: 17000763
    43. A general view of the NS-target genes indicates the possible pathways in which NS plays its role in proliferation control in Hela cells PMID: 17310849
    44. These results demonstrate that, in the cells investigated, the level of NS is regulated by p14(ARF) and the control of the G1/S transition by NS operates in a p53-dependent manner. PMID: 17494866
    45. may be involved in carcinogenesis of the cervix and progression of cervical carcinoma PMID: 18419830
    46. These results suggest that a p53-dependent cell cycle checkpoint monitors changes of cellular NS levels via the impediment of MDM2 function. PMID: 18426907
    47. Nucleophosmin is a binding partner of nucleostemin in human osteosarcoma cells PMID: 18448670
    48. Nucleostemin expression in cardiomyocytes is induced by fibroblast growth factor-2 and accumulates in response to Pim-1 kinase activity. PMID: 18519946
    49. nucleoplasmic relocation of nucleostemin during nucleolar disassembly safeguards the G2-M transit and survival of continuously dividing cells by MDM2 stabilization and p53 inhibition. PMID: 19033382
    50. a novel role of nucleostemin in ribosome biogenesis. PMID: 19106111

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  • 亞細(xì)胞定位：
    Nucleus. Nucleus, nucleolus.
  • 蛋白家族：
    TRAFAC class YlqF/YawG GTPase family
  • 組織特異性：
    Increased levels in lung tissue in cancer patients.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 29931

    OMIM: 608011

    KEGG: hsa:26354

    STRING: 9606.ENSP00000395772

    UniGene: Hs.313544