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  4. Recombinant Human Histone H3.1t (HIST3H3)

Recombinant Human Histone H3.1t (HIST3H3)

  • 中文名稱：
    人HIST3H3重組蛋白
  • 貨號：
    CSB-YP623092HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人HIST3H3重組蛋白
  • 貨號：
    CSB-EP623092HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人HIST3H3重組蛋白
  • 貨號：
    CSB-EP623092HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人HIST3H3重組蛋白
  • 貨號：
    CSB-BP623092HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人HIST3H3重組蛋白
  • 貨號：
    CSB-MP623092HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HIST3H3
  • Uniprot No.：
  • 別名：
    HIST3H3; H3FT; Histone H3.1t; H3/t; H3t; H3/g
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    2-136
  • 氨基酸序列
    ARTKQTARK STGGKAPRKQ LATKVARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LMREIAQDFK TDLRFQSSAV MALQEACESY LVGLFEDTNL CVIHAKRVTI MPKDIQLARR IRGERA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能參考文獻：
    1. Data show that phosphatase and tensin homolog (PTEN) interacts with death domain associated protein (DAXX) and, in turn PTEN directly regulates oncogene expression by modulating DAXX-histone H3.3 (H3.3) association on the chromatin. PMID: 28497778
    2. Data suggest TCF19 interacts with histone 3 lysine 4 trimethylation through its plant homeodomain finger; TCF19 expression appears to regulate gluconeogenesis in hepatocytes; TCF19 interacts with CHD4 causing NuRD complex recruitment to gene promoters of enzymes involved in gluconeogenesis. (TCF19 = transcription factor 19; CHD4 = chromodomain helicase DNA binding protein 4; NuRD = nucleosome-remodeling-deacetylase) PMID: 29042441
    3. Data suggest that, during monocyte-into-macrophage differentiation, extensive trimethylation of histone H3 lysine 4 as well as histone H3 lysine 27 promotes occupancy of histone H3 at promoters of transcription factors such as HOXA (homeodomain proteins) and FOXO (forkhead transcription factors). PMID: 28304152
    4. Data suggest post-translational modifications of histones, trimethylation of lysine 36 in H3 (H3K36me3) and acetylation of lysine 16 in H4 (H4K16ac), have roles in DNA damage repair; H3K36me3 stimulates H4K16ac upon DNA double-strand break; SETD2, LEDGF, and KAT5 are required for these epigenetic changes. (SETD2 = SET domain containing 2; LEDGF = lens epithelium-derived growth factor; KAT5 = lysine acetyltransferase 5) PMID: 28546430
    5. Data show that tet oncogene family member 2 (TET2) cysteine-rich (CR) domain mutations disrupt the recognition of histone H3 lysine 36 (H3K36) methylation, its cellular localization, and enzyme activity. PMID: 28130413
    6. Screening for H3.3 G34 mutation should therefore be recommended as a routine diagnostic marker for supratentorial central nervous system tumors PMID: 26482474
    7. Data suggest chromatin exhibits histone 3 methylation markers (H3K4me3, H3K27me3) in fetal brain, heart, and liver, probably to keep progenitor cells in these organs ready for immediate differentiation. (study of 12-week aborted embryo tissues, China) PMID: 26719341
    8. Regulators of the histone H3-trimethyl lysine-4 (H3K4me3) mark are significantly associated with the genetic risk architecture of common neurodevelopmental disease. PMID: 26575220
    9. EP400 deposits H3.3 into chromatin alongside H2AZ and contributes to gene regulation after Pol II pre-initiation complex assembly. PMID: 26669263
    10. Concurrent acetylation and methylation at H3K27 occurs in hepatocellular carcinoma cells in association with p53 abnormalities. PMID: 24614346
    11. TIP60 interacted with H3K4me3 in response to TNF-alpha signaling. PMID: 25560918
    12. Our data highlights the complex interplay between Nrf2 and H3S10 phosphorylation in arsenite-activated HO-1 transcription. PMID: 26291278
    13. This study showed that Pediatric brainstem oligodendroglial tumors can include histone H3.3-mutated tumors and have a tendency to disseminate throughout the neuroaxis at the time of relapse. PMID: 25281433
    14. Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications. PMID: 25519718
    15. Histone H3.3. mutations drive pediatric glioblastoma through upregulation of MYCN. PMID: 23539269
    16. our data show that histone variant H3.3 occupies distinct intranuclear chromatin domains and that these genomic loci are associated with gene expression PMID: 25482197
    17. Findings suggest a role of checkpoint kinase 1 (CHK1) as an histone H3.3 serine 31 kinase. PMID: 25690891
    18. histone H3 lysine 9 acetylation has a dual role in human embryonic stem cell pluripotency and neural differentiation PMID: 25519907
    19. Posttranslational modifications of H3 histone (methylation) does not correlate with the sox2 expression in malignant gliomas. PMID: 25696994
    20. Reptin and Pontin oligomerization and activity are modulated through histone H3 N-terminal tail interaction. PMID: 25336637
    21. Deregulation of histone H3 trimethylation at lysine 27 in diffuse large B-cell lymphoma may be another high-risk phenotype PMID: 25149548
    22. Data indicate that S6 kinase 2 (S6K2) can phosphorylate histone H3 at position Thr45, which may play a role during cell proliferation and/or differentiation. PMID: 23564320
    23. Our data suggest that the modification and release of histones could serve markers of apoptosis in human cancer cells. PMID: 24952159
    24. The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations. PMID: 24324728
    25. Data suggest riboflavin (RF) status regulates hepatocyte gene expression; in HepG2 cells, depletion of LSD1 (lysine (K)-specific demethylase 1A) causes aberrant gene regulation in RF-deficient cells via changes in H3 methylation at albumin promoter. PMID: 24744315
    26. full-length PHF1 in HEK293 cells co-localizes with histone K27me3, but not with K36me3, and this co-localization depends on the trimethyllysine binding pocket indicating that K27me3 is an in vivo target for the PHF1 Tudor domain PMID: 23954330
    27. H3K4 monomethylation establishes boundaries that restrict the recruitment of chromatin-modifying enzymes to defined regions within promoters. PMID: 24656132
    28. Data indicate that MLL1 methylates Ash2L in the absence of histone H3, but only when assembled within a complex with WDR5 and RbBP5. PMID: 24235145
    29. Disruption of neocortical histone H3 homeostasis by soluble Abeta implicates Alzheimer's disease. PMID: 23582659
    30. Ki-67 staining is stronger than PHH3, making 'hot spots' easier to identify on ACIS. Ki-67 is more ideal than PHH3( Phosphohistone H3 ) for staining NENs, especially in tumors with borderline grades. PMID: 24021213
    31. histone H3 lysine 79 dimethylation (H3K79me2) and a novel identified site, H2bK5 monomethylation (H2bK5me1), were completely absent in individuals with Neural tube defects PMID: 23376398
    32. PRAME expression in leukaemic cell lines is upregulated by IFN gamma and LPS, suggesting a possible role in immune responses. Nuclear PRAME interacts with Histone H3, suggesting a role in gene regulation in the nucleus. PMID: 23460923
    33. Menin-dependent transcriptional repression of histone H3 lysine 9 methylation might play an important role in preventing tumors. PMID: 23579270
    34. Data using recombinant proteins in cell-free lysates suggest that interaction of survivin with phosphorylated histone 3 (H3T3-Phos; a mitotic biological marker appearing during cell division) is abolished by trimethylation at neighboring lysine. PMID: 23281010
    35. SIRT2-mediated H3K18 deacetylation plays a critical role during infection, which reveals an epigenetic mechanism imposed by a pathogenic bacterium to reprogram its host. PMID: 23908241
    36. The SUV39H1 chromodomain was shown to recognize histone H3K9me2/3 specifically. PMID: 23285239
    37. Results suggest that histone modification in H3K27 detected using immunohistochemistry can be successfully used as an independent prognostic factor for colorectal cancer patients with metachronous liver metastasis. PMID: 23523318
    38. C-kinase-activated protein phosphatase (CPI)-17 inhibitor knockdown in human pancreatic cancer cells results in dephosphorylation of histone H3. PMID: 23541585
    39. Results suggest that GLP may play a significant role in the maintenance of HIV-1 latency by catalyzing dimethylation of H3K9. PMID: 23541084
    40. Study shows that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex formation. PMID: 23452851
    41. Histone H3 Ser-10 phosphorylation can be designated a new 'apoptotic histone code' mediated by PKCdelta. PMID: 22984491
    42. Histone H3 is increased during amino acid response that is associated with active transcription. PMID: 22978410
    43. The levels of H3K4me3 and H3K27me3 show dynamic changes during human oocyte maturation and preimplantation embryonic development. PMID: 22818287
    44. Increased levels of decondensed chromatin in both normal progenitor cells and cancer cells are associated with global loss of H3K27me3, which is linked to MYC overexpression. PMID: 22713676
    45. Elongated telomeres show increased trimethylated histone H3 Lys9 (H3K9me3)density. PMID: 22922742
    46. 17beta-estradiol stimulation induces the recruitment of PAD2 to target promoters by ERalpha, whereby PAD2 then citrullinates H3R26, which leads to local chromatin decondensation and transcriptional activation. PMID: 22853951
    47. Trimethylation of lysine 27 on histone H3 expression, as examined by immunohistochemistry, has the potential to be used as an immunomarker to predict nasopharyngeal carcinoma chemoradiotherapy response and patient prognosis. PMID: 21738951
    48. aberration of the global H3K9me2 level is an important epigenetic event in colorectal tumorigenesis and carcinogenesis PMID: 21917293
    49. our data suggest that global histone H3 and H4 modification patterns are potential markers of tumor recurrence and disease-free survival in non-small cell lung cancer PMID: 22360506
    50. O-GlcNAcylation regulates mitosis-specific phosphorylations on H3, providing a mechanistic switch that orchestrates the G2-M transition of the cell cycle. PMID: 22371497

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H3 family
  • 組織特異性：
    Expressed in testicular cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4778

    OMIM: 602820

    KEGG: hsa:8290

    STRING: 9606.ENSP00000355657

    UniGene: Hs.248171