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Recombinant Human Histone H3.2 (HIST2H3A)

  • 中文名稱：
    人HIST2H3A;重組蛋白
  • 貨號：
    CSB-YP744479HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人HIST2H3A;重組蛋白
  • 貨號：
    CSB-EP744479HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人HIST2H3A;重組蛋白
  • 貨號：
    CSB-EP744479HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人HIST2H3A;重組蛋白
  • 貨號：
    CSB-BP744479HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人HIST2H3A;重組蛋白
  • 貨號：
    CSB-MP744479HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HIST2H3A;
  • Uniprot No.：
  • 別名：
    HIST2H3A;; HIST2H3C; H3F2; H3FM;; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    2-136
  • 氨基酸序列
    ARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEASEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能參考文獻：
    1. Finally, mutations of H3G34 or H3P38 alleviate the inhibitory effects of H3K36M on H3K36 methylation, demonstrating that the stable interaction of H3K36M with SETD2 is critical for its inhibitory effects. PMID: 28256625
    2. Studies presented here confirm that the adenovirus 2 large E1A protein activation domain interacts with p300, as reported previously (P. Pelka, J. N. G. Ablack, J. Torchia, A. S. Turnell, R. J. A. Grand, J. S. Mymryk, Nucleic Acids Res 37:1095-1106, 2009), and that the resulting acetylation of H3K18/27 affects varied steps in transcription at different viral promoters. PMID: 29976669
    3. This study presented the H3 K27M mutations in posterior fossa ependymomas. PMID: 27539613
    4. Findings point to epigenetic mark H3K27me3 as an important event in prostate carcinogenesis and progression. PMID: 28403887
    5. Study identified phosphorylation of histone H3 at serine 10 (H3S10ph) as a histone post-translational modifications with most consistent and significant difference between gastric cancer (GC) tumor and negative resection margin. H3S10 phosphorylation in GC is mediated by MSK1 through the p38- MAPK pathway. PMID: 27588146
    6. Results showed that high levels of MMSET in the myeloma-like cells drove the formation of hypermethyled proteoforms containing H3K36me2 co-existent with the repressive marks H3K9me2/3 and H3K27me2/3. PMID: 26272979
    7. Results identified histone H3K27me3 as elevated in aggressive melanoma cells relative to less aggressive melanoma cells in cell culture and in patient tissues. PMID: 26621846
    8. Study shows proteolytic processing of H2B and H3 in hepatocytes where four different clipping sites were localized in H3 and one in H2B. Also, clipped H3 carry distinct co-existing post-translational modifications but different from those in intact H3. PMID: 26424599
    9. Locally generated fumarate inhibits KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 Lys 36. PMID: 26237645
    10. In vitro clipping assays confirmed the ability of both Neisseria meningitidis proteins, App and MspA, to proteolytically cleave the core histone H3. PMID: 25600171
    11. H3T80ph, protruding from the nucleosome surface, promotes interactions between adjacent nucleosomes to promote chromatin compaction during mitosis in metazoan cells. PMID: 24275038
    12. UHRF1 PHD finger (including the preceding zinc-Cys4 knuckle) acts together with the adjacent double Tudor domain to specifically recognize the H3K9me3 mark. PMID: 22100450
    13. NASP balances the activity of the heat shock proteins Hsc70 and Hsp90 to direct H3-H4 for degradation by chaperone-mediated autophagy PMID: 22195965
    14. purification of a novel JmjC domain-containing protein, JHDM1 (JmjC domain-containing histone demethylase 1), that specifically demethylates histone H3 at lysine 36 PMID: 16362057
    15. BirA ligase biotinylates K4, K9, K18 and K23 in histone H3. PMID: 18452652
    16. Sustained JNK1 activation is associated with altered histone H3 methylations in human liver cancer. PMID: 19041150
    17. phosphorylation of H3T45 (H3T45ph) increases dramatically in apoptotic cells, around the time of DNA nicking PMID: 19363025
    18. The acetylation of lysine(56) residue of Histone H3 is critical for genomic stability and DNA damage response. PMID: 19411844
    19. Acetylation of H3 K56 does not directly affect the compaction of chromatin and has modest effects on chromatin remodeling. However, H3 K56 acetylation increases DNA breathing 7-fold. PMID: 19818718

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H3 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 20505

    OMIM: 142780

    KEGG: hsa:126961

    STRING: 9606.ENSP00000385479

    UniGene: Hs.647745