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  4. Recombinant Human Homeobox protein OTX2 (OTX2)

Recombinant Human Homeobox protein OTX2 (OTX2)

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  • 中文名稱：
    人OTX2重組蛋白
  • 貨號(hào)：
    CSB-EP017299HU
  • 規(guī)格：
    ￥1344
  • 促銷：
    現(xiàn)貨重組蛋白特價(jià)促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    CPHD6; Homeobox protein OTX2; MCOPS 5; MCOPS5; MGC45000; Orthodenticle 2; Orthodenticle homeobox 2; Orthodenticle homolog 2 (Drosophila); Orthodenticle homolog 2; Orthodenticle2; Otx 2; otx2; OTX2_HUMAN
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length of Isoform 2
  • 來(lái)源：
    E.coli
  • 分子量：
    48.4kDa
  • 表達(dá)區(qū)域：
    1-297aa
  • 氨基酸序列
    MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPGPWASCPAATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPAKKKTSPAREVSSESGTSGQFTPPSSTSVPTIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

■ 常見問題解答
Q:

1. It is listed as 1-297aa, but UniProt only listed 289aa full length, could you confirm the length of the protein?
2. Since it is purified with NiNTA columns, can the final product be prepared imidazole free (or ultra low concentration)?
http://www.cusabio.com/Recombinant-Protein/Recombinant-human-Homeobox-protein-OTX2-Mammalian-cell-12547833.html

A:
Very nice to receive your inquiry. Recombinant human Homeobox protein OTX2
CSB-YP017299HU
CSB-EP017299HU
CSB-BP017299HU
CSB-MP017299HU
Expression Region: 1-297aa, full length of Isoform 2
Tag Info: EP: N-terminal 6xHis-sumo-tag; YP, BP, MP: N-terminal 6xHis-tag
Target Protein Sequence:

MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPGPWASCPAATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPAKKKTSPAREVSSESGTSGQFTPPSSTSVPTIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL


We could provide the final product free of imidazole. Please kindly remark this requirement when sending your PO.
Q:

1. Is there (a way) to make the protein with N-term His but no SUMO?
2. any changes in lead time for 5mg?
3. confirming immidazole free, and supply protein in PBS?

A:
Thanks for your inquiry. Yes. We could offer the EP protein with N-terminal His-tag, but the price and lead time will change. The product details are as below.
Code: CSB-EP017299HU
Name: Recombinant human Homeobox protein OTX2
Distributor Price: 40-50 working days
Tag Info: N-terminal 6xHis-tag
Buffer: If you would like the protein supplied in PBS, free of immidazole. Please just remark this requirement when sending your PO.
Because CSB-EP017299HU with N-terminal 6xHis-sumo-tag is a stock protein, so the price is relatively cheaper.
The lead time will be 25-30 working days.
Q:

Can you calculated the molar concentration of the protein we delivered?

A:
The molar concentration of CSB-EP017299HU is 0.037mol/L.
Q:

I have a few questions regarding the Catalog # CSB-EP017299HU Recombinant Human Homeobox protein OTX2(OTX2):
1. Is there a version of the protein with only N-terminal 6xHis tag?
2. Can the protein be supplied in PBS instead of Tris-based buffer?
3. My downstream application requires that the protein is in imidazole-free buffer. Does the protein solution go through dialysis steps to remove imidazole from elution buffer?

A:
Very nice to receive your inquiry.
1. For CSB-EP017299HU, we could also provide N-terminal 6xHis tag. Because CSB-EP017299HU with N-terminal 6xHis-sumo-tag is a stock protein, so the price will be relatively cheaper.
2. If the customer would like the protein supplied in PBS, free of immidazole. Please just remark this requirement when sending your PO.

靶點(diǎn)詳情

  • 功能：
    Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.
  • 基因功能參考文獻(xiàn)：
    1. This second report of maculopathy associated with a heterozygous mutation in OTX2 confirms that mutations in OTX2 should be considered in the differential diagnosis of atypical hereditary maculopathy, with or without rod-cone dystrophy. PMID: 29204067
    2. OTX2 acts as a pioneer factor and, in cooperation with NEUROD1, controls the group 3 medulloblastoma active enhancer landscape. PMID: 28213356
    3. Syndromic microphthalmia due to mutations in OTX2 can present with significant intrafamilial phenotypic variability. PMID: 28388256
    4. We report a rare case of inherited 14q22.3 deletion in a very large family presenting with variable features of eye abnormalities (microphthalmia, anophthalmia and peripapillary pigmentation), pituitary anomalies, and learning difficulties. PMID: 26860946
    5. Our data refine the clinical spectrum associated with OTX2 mutations and suggests that OTX2 haploinsufficiency should be considered as a possible cause for isolated mandibular dysostosis. PMID: 27378064
    6. One subgroup of atypical teratoid rhabdoid tumors was characterized by high expression of OTX2. PMID: 28025236
    7. TNF-alpha, secreted from activated Monocytes, mediates the downregulation of OTX2 and essential retinal pigment epithelium genes. PMID: 27660103
    8. OTX2 gene frameshift mutation is associated with microphthalmia, ectopic pituitary and growth hormone deficiency. PMID: 26974134
    9. OTX2 mutations can cause ACTH deficiency in the neonatal period. Study also shows that OTX2 mutations are associated with agenesis of the Left Internal Carotid Artery. PMID: 27299576
    10. A novel pathogenic variant in OTX2, c.651delC, p.(Thr218Hisfs*76) identified in a patient with syndromic bilateral anophthalmia . PMID: 26130484
    11. demonstrated the role of OTX2 on TAp63 regulation necessary for the correct formation of macular neuroepithelium PMID: 26554466
    12. A novel role for OTX2 in self-renewal and migration of human embryonic neural precursors and Medulloblastoma cells, is reported. PMID: 26398939
    13. We showed that miR-410 directly regulates predicted target genes OTX2 and RPE65. PMID: 25351180
    14. This epigenome-wide DNA methylation analysis in postmortem hippocampus and prefrontal cortex specimens confirmed OTX2 DNA methylation profiles in major depression PMID: 25571874
    15. OTX2 overexpression is associated with medulloblastoma. PMID: 25198066
    16. SOX2, OTX2 and PAX6 analysis in subjects with anophthalmia and microphthalmia PMID: 25542770
    17. Agnathia-otocephaly complex and asymmetric velopharyngeal insufficiency due to an in-frame duplication in OTX2. PMID: 25589041
    18. OTX2 mutations cause autosomal dominant pattern dystrophy of the retinal pigment epithelium. PMID: 25293953
    19. Endogenous OTX2 from a medulloblastoma cell line is O-GlcNAcylated at several sites. PMID: 24580054
    20. a role for OTX2 dosage sensitivity in human craniofacial development PMID: 24816892
    21. VEGF-A, Otx2 homeobox and p53 family genes are expressed in proliferative vitreoretinopathy PMID: 24227910
    22. OTX2 gene duplication is associated with autosomal dominant oculoauriculovertebral syndrome. PMID: 23794319
    23. This study showed that OTX2 sustains a bivalent-like state of OTX2-bound promoters in medulloblastoma by maintaining their H3K27me3 levels. PMID: 23179372
    24. Otx2c is a novel alternative splicing variant of Otx2 gene and it is tightly regulated during the differentiation process demonstrating that it may have a potential role in embryonic stem cell maintenance and differentiation. PMID: 23566845
    25. OTX2 functions via its homeobox domain as a suppressor of differentiation, and the loss of OTX2 expression is linked to the myogenesis in medullomyoblastoma. PMID: 22986744
    26. This study reports a large family in which two cousins with micro/anophthalmia each gave birth to at least one child with otocephaly. The study subsequently identified in one unrelated otocephalic patient a sporadic OTX2 mutation. PMID: 22577225
    27. We have identified a novel mutation of the OTX2 gene in a Han Chinese family featuring various anophthalmia and microphthalmia phenotypes. PMID: 22268617
    28. A novel missense heterozygous OTX2 mutation acts as a dominant negative inhibitor of target gene expression in a patient with combined pituitary hormone deficiency (CPHD), pituitary malformation, and optic nerve hypoplasia. PMID: 22715480
    29. MiR-206 regulates Otx2 expression in glioma and neuroblastoma cell lines. PMID: 22508046
    30. silencing of OTX2 inhibited cell proliferation and resulted in a neuronal-like differentiation; downregulated genes were enriched for cycle and visual perception genes; upregulated genes were enriched for genes involved development and differentiation PMID: 21964830
    31. There is no genotype-phenotype correlation and the severity of the disease varies not only by the specific OTX2 mutations but also among individuals harboring the same mutation, suggesting the involvement of multiple levels of regulation. PMID: 22783640
    32. these findings emphasize the role of OTX2 in regulating the human TYR gene, with implications for inter-individual differences in melanin synthesis, retinal development, and function as well as susceptibility to retinal degeneration associated with aging. PMID: 22259223
    33. Mutation analysis was performed after sequencing the entire coding regions of OTX2 and PRRX1 genes isolated from the proband and his parents. After thorough analysis, no DNA variations were detected PMID: 22198066
    34. The OTX2 mutation in c.313C > T causing p.Gln105X and a premature stop codon is consistent with the loss of function mutations observed in A/M patients. PMID: 22204637
    35. OTX2 directly induces a series of cell cycle genes but requires cooperating genes for an oncogenic acceleration of the cell cycle. PMID: 21047732
    36. This high frequency of causal submicroscopic chromosomal aberrations in patients with congenital ocular malformation warrants implementation of array comparative genomic hybridization in the diagnostic work-up of these patients. PMID: 21353197
    37. Four of the five OTX2-positive anophthalmia/microphthalmia patients in our study displayed additional systemic findings, including two novel features, Wolf-Parkinson-White syndrome and an anteriorly placed anus PMID: 20486942
    38. Otx2 is required to specify neuron subtype in ventral tegmental area and confers resistance to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-HCl (MPTP) poisoning. PMID: 21057506
    39. SOX9, through interaction with microphthalmia-associated transcription factor (MITF) and OTX2, regulates BEST1 expression in the retinal pigment epithelium. PMID: 20530484
    40. OTX2 overexpression protects dopaminergic neurons in ventral mesencephalic cultures from Parkinson's disease-relevant toxin, 1-methyl-4-phenylpyridinium, whereas OTX2 downregulation using short hairpin RNA increases their susceptibility. PMID: 20573704
    41. The early expression of OTX2 in proliferative cell layers of the human fetal brain supports the concept that this homeobox gene is important in neuronal cell development and differentiation. PMID: 20354145
    42. A novel loss-of-function mutation in OTX2 in a patient with anophthalmia and isolated growth hormone deficiency. PMID: 20396904
    43. OTX2 mutations are associated with variable pituitary phenotype, with no genotype-phenotype correlations, and that OTX2 can transactivate GNRH1 as well as HESX1 and POU1F1. PMID: 19965921
    44. Our findings define OTX2 as an important oncogenic driver in medulloblastoma. PMID: 20028867
    45. Data suggest that OTX2 may regulate retinal pigment epithelium (RPE)-specific target genes, such as DOPAchrome tautomerase (DCT), thereby maintaining the homeostasis of RPE. PMID: 12559959
    46. OTX2 functions to specify the fate of neuroectoderm in various regions of the developing brain. This developmental role is consistent with the evidence suggesting that OTX2 is a medulloblastoma oncogene. PMID: 15705863
    47. Gene expression analyses showed that OTX2 transcripts were present at high levels in 14 of 15 (93%) medulloblastomas with anaplastic histopathologic features. PMID: 15705891
    48. Heterozygous mutations of OTX2 cause severe ocular malformations. PMID: 15846561
    49. Molecular diagnostic of human mutant OTX2 proteins discriminates hypomorphic and loss of function mutations from other mutations that may not be relevant to ocular pathology PMID: 16607563
    50. This review describes how cross regulation for PAX6, SOX2 and perhaps OTX2 has now been uncovered, pointing to the mechanisms that can fine-tune the expression of three such essential components in eye development. PMID: 16712695

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  • 相關(guān)疾病：
    Microphthalmia, syndromic, 5 (MCOPS5); Pituitary hormone deficiency, combined, 6 (CPHD6); Retinal dystrophy, early-onset, with or without pituitary dysfunction (RDEOP)
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Paired homeobox family, Bicoid subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 8522

    OMIM: 600037

    KEGG: hsa:5015

    STRING: 9606.ENSP00000343819

    UniGene: Hs.288655