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  4. Recombinant Human Ileal sodium/bile acid cotransporter (SLC10A2), partial

Recombinant Human Ileal sodium/bile acid cotransporter (SLC10A2), partial

  • 中文名稱：
    人SLC10A2重組蛋白
  • 貨號：
    CSB-YP622519HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人SLC10A2重組蛋白
  • 貨號：
    CSB-EP622519HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人SLC10A2重組蛋白
  • 貨號：
    CSB-EP622519HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人SLC10A2重組蛋白
  • 貨號：
    CSB-BP622519HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人SLC10A2重組蛋白
  • 貨號：
    CSB-MP622519HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC10A2
  • Uniprot No.：
  • 別名：
    Apical sodium dependent bile acid transporter; Apical sodium- dependent bile acid transporter; Apical sodium-dependent bile acid transporter; ASBT; IBAT; ileal; ileal apical sodium-dependent bile acid transporter; Ileal Na(+)/bile acid cotransporter; Ileal sodium dependent bile acid transporter; Ileal sodium-dependent bile acid transporter; Ileal sodium/bile acid cotransporter; ISBT; Na(+)-dependent ileal bile acid transporter; Na+ bile acid cotransporter; Na+ dependent ileal bile acid transporter; NTCP2; NTCP2_HUMAN; PBAM; SLC10A2; Sodium/taurocholate cotransporting polypeptide; Sodium/taurocholate cotransporting polypeptide; ileal; solute carrier family 10 (sodium/bile acid cotransporter family); Solute carrier family 10 (sodium/bile acid cotransporter family); member 2; Solute carrier family 10 member 2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism.
  • 基因功能參考文獻：
    1. A cysteine-less form of hASBT was made by creating point mutations at all 13 endogenous cysteines. Cysless hASBT had significantly reduced function correlated with lowered surface expression.Chemical cross-linking of wild-type and Cysless species revealed that hASBT has a dominant negative effect and exists as an active dimer and/or higher order oligomer with apparently no requirement for endogenous cysteine residues. PMID: 29198943
    2. Two single-nucleotide polymorphisms at novel loci, rs112404845 (P = 3.8 x 10-8), upstream of COBL, and rs16961023 (P = 4.6 x 10-8), downstream of SLC10A2, obtained genome-wide significant evidence of association with the posterior liability of late-onset Alzheimer's disease in African Americans. PMID: 27770636
    3. Data (including data from studies in knockout/transgenic mice) suggest that SLC10A2 is a functional receptor for hepatitis D virus in hepatocytes. PMID: 25902143
    4. Results unravel novel roles for N-glycosylation of ASBT and suggest that high levels of glucose alter the composition of the glycan and may contribute to the increase in ASBT function in diabetes mellitus. PMID: 25855079
    5. The p.Ser267Phe NTCP variant is significantly associated with resistance to chronic hepatitis B and a lower incidence of acute-on-chronic liver failure. Our results support that NTCP is a cellular receptor for HBV in human infection PMID: 25418280
    6. Data indicate that the lipid flippase (ATP8B1)-transmembrane protein 30A (CDC50A) heterodimer is essential for the apical localization of sodium-dependent bile acid transporter (SLC10A2/ASBT) in Caco-2 cells. PMID: 25239307
    7. It was conclude that regulation of ASBT expression by resveratrol (RSV) may have clinical relevance with regard to the observed cholesterol-lowering effects of RSV. PMID: 24498857
    8. Transmembrane domain II of the human bile acid transporter SLC10A2 coordinates sodium translocation. PMID: 24045943
    9. ASBT evolved from the earliest vertebrates by gaining affinity for modern bile salts while retaining affinity for older bile salts PMID: 22669917
    10. This study provided novel evidence for the alterations in the activity of ASBT by enteropathogenic Escherichia coli infection. PMID: 22403793
    11. There was no significant association of rs9514089 with gallstone risk, serum lipid parameters and BMI in the Sorbs and in the meta-analysis of all three cohorts. [meta-analysis] PMID: 22093174
    12. Presence of multiple functionally relevant variants in SLC10A2 that may influence bile acid homeostasis and physiology. PMID: 21649730
    13. The human ASBT promoter was activated transcriptionally by CDX1 and CDX2. PMID: 22016432
    14. The beneficial effect of rifampicin in cholestasis is associated with an increase in DME expression involved in toxic, bile acid and cholesterol metabolism, as well as a reduction in the bile acid importing system in hepatocytes. PMID: 21526375
    15. data demonstrate that TM1 plays a pivotal role in ASBT function and stability, thereby providing further insight in its dynamic transport mechanism PMID: 21646357
    16. the essential role of ASBT in the uptake of bile acids, by which the enterohepatic recirculation of bile acids is maintained PMID: 21341987
    17. Results show that bile acid conjugates are potential prolonged release prodrugs with binding affinity for ASBT. PMID: 20600720
    18. These data confirm that bile acids and upregulation of ASBT play a crucial role in NEC pathogenesis and suggest that inhibition of ASBT could be utilized as a therapeutic modality against this disease. PMID: 20616306
    19. Data demonstrate a novel role of lipid rafts in the modulation of ASBT function by the dietary component EGCG, which may underlie the hypocholesterolemic effects of green tea. PMID: 20056894
    20. SLC10A2 is a novel susceptibility gene for cholelithiasis in humans PMID: 19823678
    21. There was no apparent correlation between the SLC10A2 polymorphisms and bile acid production or turnover in familial hypertriglyceridemia patients. PMID: 11742882
    22. reglation of the ASBT gene by PPARalpha PMID: 12055195
    23. role in transport of bile acids in multidrug-resistance-protein 3-overexpressing cells PMID: 12220224
    24. Positively regulated by retinoic acid. Bile acids induce negative feedback regulation of human ASBT via farnesoid X-receptor-mediated, short heterodimer partner-dependent effect upon retinoic acid receptor/retinoid X receptor activation of ASBT. PMID: 15239098
    25. SLC10A2 expression is regulated by the ubiquitin-proteasome pathway PMID: 15304498
    26. On the basis of seven transmembrane topology, a three-dimensional model of ASBT is built. The model agrees with available data for pathological mutation P290S because the mutant model after in silico mutagenesis loses the ability to bind bile acids. PMID: 15350125
    27. BIle acid-methanethiosulfonyl can serve as novel and powerful tools to probe the role of endogenous as well as engineered Cys residues in the bile acid binding region(s) of hASBT. PMID: 15952798
    28. current data provide the first evidence that I-BABP is functionally associated with FXR and IBAT in the nucleus and on the membrane, respectively, stimulating FXR transcriptional activity and the conjugated bile acid uptake mediated by IBAT in the ileum PMID: 16230354
    29. ASBT and ILBP protein were 48% and 67% lower in normal weight gallstone carriers than in controls (P < 0.05); similar differences were found for mRNA expression levels. PMID: 16237211
    30. this study determines a 7TM topology for hASBT and refutes the previously proposed 9TM model PMID: 16411770
    31. hASBT-mediated prodrug targeting is discussed, including QSAR, in vitro models for hASBT assay, and the current progress in utilizing hASBT as a drug delivery target. PMID: 16749855
    32. Although the presence of a single negative charge was not essential for interaction with hASBT, monoanionic conjugates are favored for hASBT-mediated transport compared to cationic and zwitterionic conjugates. PMID: 16749860
    33. These data suggest that transmembrane segment 7 (TM7) plays a dominant role in the hASBT translocation process. PMID: 16899538
    34. One or more constituents of human serum stimulate ASBT gene expression largely via the down-stream AP-1 response element. PMID: 17942302
    35. Extracellular loop 3 amino acids are essential for human ASBT activity as primary substrate interaction points using long-range electrostatic attractive forces. PMID: 18028035
    36. cholesterol content of lipid rafts is essential for the optimal activity of ASBT PMID: 18063707
    37. involvement of transmembrane domain 4 (TM4) of hASBT in forming the putative translocation pathway. TM4 has distinct helical face that contains residues critical for transport & conformational stability. PMID: 18311924
    38. analysis of how conserved aspartic acid residues lining the extracellular loop 1 of sodium-coupled bile acid transporter ASBT interact with Na+ and 7alpha-OH moieties on the ligand cholestane skeleton PMID: 18508772
    39. Common variants of the SLC10A2 gene are not associated with sporadic or familial colorectal cancer PMID: 18644122
    40. dysfunction, and impaired adaptive responses of several of the bile acid transporters, e.g. BSEP and ASBT, results in liver and intestinal disease PMID: 18668439
    41. Cyclic AMP-mediated phosphoinositide-3-kinase{ISBT)-independent activation of Rab4 facilitates Ntcp translocation in a hepatoma cell line. PMID: 18688880
    42. haplotype carriers with the minor allele exhibited significant reduced ileal SLC10A2 expression on mRNA levels (2.6-fold, P = 0.0009) and protein levels (2.4-fold, P = 0.0157) PMID: 19184108
    43. Integration of results for transmembrane (TM)3 and TM7 cysteine mutants suggests a putative scenario to describe substrate entry and exit into the ASBT permeation pathway during translocation mechanisms. PMID: 19653651

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  • 相關(guān)疾?。?/div>
    Primary bile acid malabsorption (PBAM)
  • 亞細胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Bile acid:sodium symporter (BASS) (TC 2.A.28) family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 10906

    OMIM: 601295

    KEGG: hsa:6555

    STRING: 9606.ENSP00000245312

    UniGene: Hs.194783