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Recombinant Human Kynurenine 3-monooxygenase (KMO)

  • 中文名稱：
    人KMO重組蛋白
  • 貨號：
    CSB-EP012475HU
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    KMO
  • Uniprot No.：
  • 別名：
    dJ317G22.1; kmo; KMO_HUMAN; Kynurenine 3 hydroxylase; Kynurenine 3 monooxygenase (kynurenine 3 hydroxylase); Kynurenine 3 monooxygenase; Kynurenine 3-hydroxylase; Kynurenine 3-monooxygenase; RP1-317G22.1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    71.8kDa
  • 表達(dá)區(qū)域：
    1-486aa
  • 氨基酸序列
    MDSSVIQRKKVAVIGGGLVGSLQACFLAKRNFQIDVYEAREDTRVATFTRGRSINLALSHRGRQALKAVGLEDQIVSQGIPMRARMIHSLSGKKSAIPYGTKSQYILSVSRENLNKDLLTAAEKYPNVKMHFNHRLLKCNPEEGMITVLGSDKVPKDVTCDLIVGCDGAYSTVRSHLMKKPRFDYSQQYIPHGYMELTIPPKNGDYAMEPNYLHIWPRNTFMMIALPNMNKSFTCTLFMPFEEFEKLLTSNDVVDFFQKYFPDAIPLIGEKLLVQDFFLLPAQPMISVKCSSFHFKSHCVLLGDAAHAIVPFFGQGMNAGFEDCLVFDELMDKFSNDLSLCLPVFSRLRIPDDHAISDLSMYNYIEMRAHVNSSWFIFQKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKKVINKGLFFLGSLIAISSTYLLIHYMSPRSFLRLRRPWNWIAHFRNTTCFPAKAVDSLEQISNLISR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The preparation of Recombinant Human KMO protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This KMO was expressed in E.coli at and fused with N-terminal 6xHis-SUMO tag. According to SDS-PAGE, the purity turns out to be 90%+.

    kynurenine 3-monooxygenase (KMO) functions as a pivotal enzyme in the main branch of the enzymatic cascade because it generates several toxic metabolites, including 3-hydroxykynurenine and quinolinic acid, which are responsible for neurodegenerative and inflammatory disorders. In recent years, researchers first revealed a novel enzymatic activity independent function of KMO in triple negative breast cancer (TNBC). Specifically, the findings suggested a positive correlation between the increased expression of KMO in breast cancer and TNBC and metastasis and recurrence. Besides, the contribution of KMO expression to the malignant phenotype of TNBC cells, particularly cancer stem cell (CSC) properties, but which leads to increased expression of pluripotent genes such as Nanog, Oct4, and Sox2.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).
  • 基因功能參考文獻(xiàn)：
    1. There is the association between the rs1053230 polymorphism and depression. PMID: 28139632
    2. results show the incidence of Postpartum Depression in the Chinese population to be 7.3%, with PDS characterized by increased serum 3-HydroxyKinurenine concentration and 3-HK/Kynurenine ratio, versus matched postpartum women without PDS. Furthermore, polymorphisms of Kynurenine Monooxygenase rs1053230 are significantly associated with the incidence of PDS. PMID: 28319697
    3. Our data establish the candidacy of KMO as a causal factor for changes in the kidney leading to proteinuria and indicate a functional role for KMO and metabolites of the tryptophan pathway in podocytes PMID: 27020856
    4. A comprehensive review of the molecular properties of KMO, including its kinetics, reaction mechanism, and inhibitor structure-activity relationship (SAR), is not currently available and, thus, is our focus here PMID: 26589832
    5. SiRNA knockdown of the pathway components Kynurenine 3-monooxygenase and quinolinate phosphoribosyl transferase caused cells to revert to a state of susceptibility to 3HK-mediated apoptosis. PMID: 27077813
    6. These results suggest that KMO exhibits tumor-promoting effects towards hepatocellular carcinoma (HCC) and it may serve as a novel prognostic marker in HCC. PMID: 26099564
    7. Results suggest that KMO variation influences a range of cognitive domains known to predict functional outcome in schizophrenia PMID: 25464917
    8. Two KMO SNPs were observed more often in schizophrenia patient group compared with healthy controls. PMID: 25715464
    9. findings from five independent cohorts suggest that genetic variation in KMO influences the risk for psychotic features in mania of bipolar disorder patients. PMID: 23459468
    10. Study reporting on the first successful bacterial (Escherichia coli) expression of active FLAGtrade mark-tagged human KMO enzyme expressed in the soluble fraction and progress towards its purification. PMID: 24316190
    11. analyzed association between KMO gene polymorphisms and CSF concentrations of kynurenic acid in patients with schizophrenia and controls. results suggest that the nonsynonymous KMO SNP rs1053230 influences CSF concentrations of KYNA. PMID: 21693093
    12. significant and correlated reduction in gene expression and enzyme activity in the frontal eye field in schizophrenia patients; rs2275163 has modest effects on predictive pursuit and visuospatial working memory endophenotypes PMID: 21727251
    13. The present analysis of the combined Scandinavian sample did not reveal any allele frequency difference between patients and healthy controls PMID: 21030213
    14. the function KMO AND indoleamine 2,3-dioxygenase may change from a role in immunosuppression at the maternal-fetal interface in early pregnancy, to one associated with regulation of fetoplacental blood flow or placental metabolism in late gestation PMID: 15950064
    15. Results suggest that kynurenine 3-monooxygenase is unlikely to be related to the development of schizophrenia in Japanese. PMID: 16716206

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  • 亞細(xì)胞定位：
    Mitochondrion outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Aromatic-ring hydroxylase family, KMO subfamily
  • 組織特異性：
    Highest levels in placenta and liver. Detectable in kidney.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6381

    OMIM: 603538

    KEGG: hsa:8564

    STRING: 9606.ENSP00000355517

    UniGene: Hs.731056