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  4. Recombinant Human Microtubule-associated protein RP/EB family member 1 (MAPRE1)

Recombinant Human Microtubule-associated protein RP/EB family member 1 (MAPRE1)

  • 中文名稱：
    人MAPRE1重組蛋白
  • 貨號：
    CSB-YP620890HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人MAPRE1重組蛋白
  • 貨號：
    CSB-EP620890HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人MAPRE1重組蛋白
  • 貨號：
    CSB-EP620890HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人MAPRE1重組蛋白
  • 貨號：
    CSB-BP620890HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人MAPRE1重組蛋白
  • 貨號：
    CSB-MP620890HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MAPRE1
  • Uniprot No.：
  • 別名：
    5530600P05Rik; Adenomatous polyposis coli binding protein EB 1; Adenomatous polyposis coli binding protein EB1; AI462499; AI504412; APC binding protein EB 1; APC binding protein EB1 ; APC-binding protein EB1; AW260097; BIM1p; D2Ertd459e; EB 1; EB1; End binding protein 1 ; End-binding protein 1; fa01e12; fc23e11; fi33c06; MAPRE 1; MAPRE1; Mapre3; MARE1_HUMAN; MGC117374; MGC129946; MGC52508 ; Microtubule associated protein RP/EB family member 1; Microtubule-associated protein RP/EB family member 1; wu:fa01e12; wu:fc23e11; wu:fi33c06; zgc:55428; zgc:77807; zgc:85755
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    2-268
  • 氨基酸序列
    AVNVYSTSV TSDNLSRHDM LAWINESLQL NLTKIEQLCS GAAYCQFMDM LFPGSIALKK VKFQAKLEHE YIQNFKILQA GFKRMGVDKI IPVDKLVKGK FQDNFEFVQW FKKFFDANYD GKDYDPVAAR QGQETAVAPS LVAPALNKPK KPLTSSSAAP QRPISTQRTA AAPKAGPGVV RKNPGVGNGD DEAAELMQQV NVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG PQEEQEEY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2. May play a role in cell migration.
  • 基因功能參考文獻：
    1. EB1 and dynein mediate migration in 3D by promoting microtubule dynamics, through the activity of RhoA, in microtubule-rich pseudopodial dendritic protrusions that drive cell migration in 3D PMID: 29097501
    2. End-binding protein 1 (EB1) depletion results in a significant reduction of spindle and kinetochore-associated protein 1 (Ska1) recruitment onto microtubules and defects in mitotic chromosome alignment. PMID: 27225956
    3. The cytotoxicity induced by EB1 gene knockdown was due to the activation and generation of reactive oxygen species by p38 mitogen-activated protein kinase..this signaling cascade, however not nuclear factor-kappaB-mediated signaling, induced the expression of cyclooxygenase-2, a key effector of apoptotic death. PMID: 29484424
    4. ASK1- induced phosphorylation of EB1 not only increases its plus end-tracking ability, but also promotes its recruitment of CLIP170 and p150glued to astral microtubules. PMID: 28039481
    5. A novel EB1 acetylation site regulates the dynamic structure of microtubules. PMID: 28777446
    6. increased peripheral EB1 distribution is a critical component of the Rac1-mediated pathway and peripheral cytoskeletal remodeling essential for agonist-induced endothelial cell barrier enhancement. PMID: 27667566
    7. In addition, EB1 phosphorylation at Y247 by Src enhances the rate of microtubule catastrophe and significantly stimulates cell migration. These findings thus demonstrate that the Src-EB1 axis plays a crucial role in regulating the crosstalk between microtubules and focal adhesions to promote cell migration. PMID: 27698945
    8. EB1 and EB3 thus affect multiple interphase processes and have a major impact on microtubule minus end organization. PMID: 28814570
    9. noncentrosomal MTs regulate autophagy through a cross-talk between CAMSAP2 and EB1 PMID: 28726242
    10. results suggest that EB1 cooperates with CDK5RAP2 and perhaps other SXIP-containing +TIPs in tracking growing microtubule tips. PMID: 28320860
    11. data provide unique structural information on the interaction of EB1 with growing microtubule ends PMID: 27617931
    12. EB1, as a microtubule plus-end tracking protein (+TIP), is a potential candidate to bridge the gap between microtubule and actomyosin dynamics. PMID: 28132723
    13. EB1 overexpression is an early event in oral tumorigenesis and cytoplasmic EB1 accumulation is associated with poor prognosis and tumor recurrence in oral squamous cell carcinoma patients PMID: 27208742
    14. We propose a novel mechanism by which ATIP3-EB1 interaction indirectly reduces the kinetics of EB1 exchange on its recognition site, thereby accounting for negative regulation of microtubule dynamic instability PMID: 26498358
    15. MAPRE1 can contribute to the detection of early-stage colorectal cancer and adenomas together with other biomarkers PMID: 26342024
    16. The authors show that the microtubule binding protein EB1 (end-binding protein 1), a key regulator of microtubule dynamics, can bind directly to filamentous actin (F-actin) F-actin. PMID: 26854759
    17. A previously uncharacterized EB1 linker region tracks microtubule plus ends in live cells. PMID: 25355949
    18. We find that the presence of EB1 can stiffen microtubules in a manner that depends on the hydrolysis state of the tubulin-bound nucleotide PMID: 25160006
    19. The functions of EB1 in the regulation of microtubule dynamics and recruitment of other +TIPs, as well as the dimerization of EB1, undergo exquisite control by phosphorylation. PMID: 25048701
    20. TTBK2 bound EB1 and Cep164 through its SxIP motifs and a proline-rich motif, respectively. PMID: 25297623
    21. We showed for the first time that EB1 associates with microtubules in a phosphorylation-dependent manner, under control of reactive oxygen species PMID: 24930764
    22. EB1 has a role in linking AIM2 inflammasomes with autophagy-dependent secretion PMID: 25164813
    23. Aptamers binding to human EB1 and EB3, which have sequence requirements similar to but distinct from each other and from Drosophila EB1, were identified. PMID: 24478452
    24. a subset of arrhythmogenic cardiomyopathy mutations, in addition to a mutation associated with skin fragility/woolly hair syndrome, impair gap junction localization and function by misregulating DP-EB1 interactions and altering microtubule dynamics. PMID: 25225338
    25. The CYLD-EB1 interaction was confirmed both in cells and in vitro, and these 2 proteins colocalized at the plus ends of microtubules. The association of CYLD with EB1 was significantly increased upon the stimulation of cell migration. PMID: 24552808
    26. Studies show that IQGAP1 functions as a hub linking HGF-induced signaling to microtubules and actin remodeling via EB1-IQGAP1-cortactin interactions. PMID: 25022754
    27. EB1-kinesin complex actively steered growing microtubules in an in vitro model PMID: 24462004
    28. Study finds that frictional forces increase nonlinearly with microtubule-associated proteins (MAP) velocity across microtubules and depend on filament polarity, with NuMA's friction being lower when moving toward minus ends, EB1's lower toward plus ends, and PRC1's exhibiting no directional preference. PMID: 24725408
    29. EB1 and EB3 proteins are obligatory dimers. PMID: 24040250
    30. EB1 enables the spindle microtubules to regulate the phosphorylation of kinetochores through recruitment of the Aurora B kinase. PMID: 24616220
    31. Dysregulation of EB1 is an important early event in colon carcinogenesis PMID: 24492008
    32. EB proteins appear to play an active role inthe subsequent association of STIM1 to APC at the ER-PM junction. PMID: 23871111
    33. regulation of EB1 dimerization might play a role in controlling EB1 function PMID: 23864329
    34. phosphorylation of STIM1 at Ser575, Ser608 and Ser621 regulates the interaction with EB1. PMID: 23687376
    35. Depletion of end-binding protein 1 (EB1) promotes apoptosis of human non-small-cell lung cancer cells via reactive oxygen species and Bax-mediated mitochondrial dysfunction. PMID: 23900080
    36. TIP150-EB1 interaction governs kinetochore microtubule plus-end plasticity and establish that the temporal control of the TIP150-EB1 interaction by PCAF acetylation ensures chromosome stability in mitosis. PMID: 23595990
    37. These observations indicate that EB1 and ch-TOG regulate microtubule organisation differently via distinct regions in the plus ends of microtubules. PMID: 23251535
    38. End binding protein 1 directly interacted with AIM2 and ASC in vitro and in vivo. PMID: 22869553
    39. the intramolecular interaction site of EB1 overlaps the MT binding sites, indicating that the MT binding sites are masked by interaction with the C-terminal domain. PMID: 23128140
    40. EB1 is a potential biomarker and therapeutic target in colorectal cancer. PMID: 22735596
    41. STIM1 dissociates from EB1 in mitosis. PMID: 22748319
    42. Data indicate that EB1 is a substrate of P300/CBP-associated factor (PCAF) and K220 was identified as a responsible site. PMID: 23001180
    43. the Amer2-EB1-APC complex regulates cell migration by altering microtubule stability. PMID: 22898821
    44. We further demonstrate that this binding was prevented when the C-terminal tyrosine of EB1 was absent in the peptidic probes. PMID: 22543185
    45. Increased plasma levels of the APC-interacting protein MAPRE1, LRG1, and IGFBP2 precede a diagnosis of colorectal cancer in women PMID: 22277732
    46. Multisite phosphorylation disrupts arginine-glutamate salt bridge networks required for binding of cytoplasmic linker-associated protein 2 (CLASP2) to end-binding protein 1 (EB1). PMID: 22467876
    47. Kif18B is a new MT dynamics regulatory protein that interacts with EB1 to control astral MT length PMID: 21737685
    48. Data indicated that EB1 and EB3 interact with proteins implicated in MT minus-end anchoring or vesicular trafficking to the cilia base, suggesting that EB1 and EB3 promote ciliogenesis by facilitating such trafficking. PMID: 21768326
    49. Insights into EB1 structure and the role of its C-terminal domain for discriminating microtubule tips from the lattice PMID: 21737692
    50. decreased MAPRE1 expression coincided with increased miR-10b expression, suggesting that miR-10b targets MAPRE1 transcription PMID: 21562367

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Golgi apparatus.
  • 蛋白家族：
    MAPRE family
  • 組織特異性：
    Ubiquitously expressed.
  • 數據庫鏈接：

    HGNC: 6890

    OMIM: 603108

    KEGG: hsa:22919

    STRING: 9606.ENSP00000364721

    UniGene: Hs.472437