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Recombinant Human Mitogen-activated protein kinase 3 (MAPK3)

  • 中文名稱：
    Recombinant Human Mitogen-activated protein kinase 3 (MAPK3)
  • 貨號(hào)：
    CSB-YP013456HU(A5)
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
  • Uniprot No.：
  • 別名：
    ERT2Extracellular domain signal-regulated kinase 1 ;ERK-1Insulin-stimulated MAP2 kinase;MAP kinase isoform p44 ;p44-MAPKMicrotubule-associated protein 2 kinasep44-ERK1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Yeast
  • 分子量：
    44.0 kDa
  • 表達(dá)區(qū)域：
    2-379aa
  • 氨基酸序列
    AAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that Thr264 in TRPV3 is key ERK1 phosphorylation site mediating EGFR-induced sensitization of TRPV3 to stimulate signaling pathways involved in regulating skin homeostasis. (TRPV3 = transient receptor potential cation channel subfamily V member-3; ERK1 = extracellular signal-regulated kinase-1; EGFR = epidermal growth factor receptor) PMID: 29084846
    2. RASSF7 promotes cell proliferation through activating MEK1/MEK2-ERK1/ERK2 signaling pathway in hepatocellular carcinoma. PMID: 29729697
    3. ERK1 Directly Interacts With JNK1 Leading to Regulation of JNK1/c-Jun Activity and Cell Transformation. PMID: 28106280
    4. the D domain of LRRC4 anchors ERK1/2 in the cytoplasm and competitively inhibits MEK/ERK activation in glioma cells. PMID: 27884160
    5. High ERK1 expression is associated with castration-resistant prostate cancer. PMID: 28844715
    6. The antitumor activity of scopoletin may be due to its strong anti-angiogenic effect, which may be mediated by its effective inhibition of ERK1, VEGF-A, and FGF-2. PMID: 27133199
    7. High ERK1 expression is associated with melanoma. PMID: 28193911
    8. findings uncover a role of ERK1 in the regulation of furin activity by supporting a self-sustaining loop for high TGF-beta activity in glioma-initiating cells. PMID: 28484053
    9. ERK1 phosphorylation is mediated by Src and Csk. PMID: 26234813
    10. Integrin beta1 appears to serve as a partner of Stathmin induction of ERK and Akt signaling by inhibiting apoptosis in the cholangiocarcinoma cell. PMID: 28178656
    11. High ERK1 expression is associated with gastric cancer. PMID: 27601158
    12. Data indicate three biomarkers mitogen-activated protein kinase 3 (MAPK3), BCL2 apoptosis regulator (BCL2) and proto-oncogene c-Akt (AKT1) as potential predictors of neurological outcome following cardiac arrest (CA). PMID: 28147324
    13. Inhibiting miR-21 attenuates hepatic fibrosis by suppressing both the ERK1 in hepatic stellate cells and epithelial-mesenchymal transition of hepatocyte. PMID: 27226339
    14. High ERK1 expression is associated with paclitaxel resistance in cervical cancer. PMID: 26810068
    15. High ERK1 expression is Associated with Nucleus Pulposus Cells' Degeneration. PMID: 27635110
    16. Low expression of ERK is associated with resistance to sorafenib in liver cancer. PMID: 26711788
    17. Increased ERK1 expression is associated with drug resistance in neoplasms. PMID: 26715278
    18. The activation of ERK1 by MEK1, subsequent slower phosphorylation of the flanking sites results in inhibition of the kinase. Because the T207 and Y210 phosphosites of ERK1 are highly conserved within the eukaryotic protein kinase family, hyperphosphorylation within the kinase activation T-loop may serve as a general mechanism for protein kinase down-regulation after initial activation by their upstream kinases. PMID: 26823016
    19. Over-expressed TWIST associates with markers of epithelial mesenchymal transition and predicts poor prognosis in breast cancers via ERK and AKT activation. PMID: 26295469
    20. p44/42, a known apoptosis-promoting regulator and caspase 3 activator, was increased in brain tumor cells treated with violacein PMID: 25816226
    21. miR-155 plays an important role in regulating the pathological network involving EMT process and ERK1 pathway during hepatic stellate cell activation. PMID: 25142507
    22. This study identified and confirmed MAPK3 protein changes within the postsynaptic density in schizophrenia PMID: 25048004
    23. Sphingosine-1-phosphate promotes extravillous trophoblast cell invasion by activating MEK/ERK/MMP-2 signaling pathways via S1P/S1PR1 axis activation. PMID: 25188412
    24. Results show that miR-483-5p expression level is up-regulated in polycystic ovary syndrome patients and inversely correlated with notch3 and MAPk3 levels and that Notch3 and MAPK3 are the direct targets of MIR483. PMID: 25622783
    25. SKLB-M8 inhibited HUVEC proliferation, migration, invasion, and tube formation in vitro with the inhibition of phosphorylated ERK1/2. PMID: 25341684
    26. Therefore, the positivity of p-ERK1/2 expression may serve as a vital biomarker in the development of non-small cell lung cancer PMID: 25596700
    27. Low ERK1 expression is associated with hormone resistance in breast cancer. PMID: 25085753
    28. Therefore, our study demonstrates that MAPK members (ERK1/2 and JNK) play a key role in CCR7 regulating SCCHN metastasis PMID: 25270024
    29. High phosphorylated ERK1 is associated with low response to chemotherapy in nonsmall-cell lung carcinoma. PMID: 25449334
    30. These results therefore indicate that p53-mediated up-regulation of MKP-3 contributes to the establishment of the senescent cellular phenotype through dephosphorylating ERK1/2 PMID: 25414256
    31. ERK1/2 signal induced MNK catalytic activity enabled enterovirus type 1 internal ribosomal entry site-mediated translation/host cell cytotoxicity through negative regulation of the Ser/Arg (SR)-rich protein kinase (SRPK). PMID: 25187541
    32. ERK, AKT, and GSK-3beta have roles in boldine-induced cell cycle arrest and apoptosis in T24 human bladder cancer cell line PMID: 24239461
    33. Insulin-induced apoptotic commitment depended on the down-regulation of Erk-1, insulin growth factor-1 receptor (IGF-1R), and fibroblast growth factor receptor-1 (FGFR-1)-mediated signaling. PMID: 24818995
    34. Enhanced t-ERK1 expression in infiltrating lymphoid cells was significantly associated with female gender, absence of vascular and perineural invasion, lymph node metastases and early depth of invasion as well as with longer disease-free survival times PMID: 24682903
    35. Expression of TMPRSS4 in gastric cancer is significantly associated with lymph node and distant metastasis, high Erk1 expression, and poor prognosis. PMID: 23922976
    36. ERK1 phosphorylates KIBRA at Ser(548) for cell proliferation and migratory activity PMID: 24269383
    37. We conclude that pERK1/2 is a sensitive marker of early colon cancer, which disappears at later stages of cancer development. PMID: 23357054
    38. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
    39. ERK1 localized to the cytosol and translocated to the nucleus upon cell activation and kinase phosphorylation. PMID: 23651922
    40. Data indicate that tocilizumab enhanced the interferon-induced phosphorylation of STAT1 and inhibited SOCS3 expression and the phosphorylation of both STAT3 and ERK. PMID: 23274199
    41. Extracellular signal-regulated kinase and glycogen synthase kinase 3beta regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism PMID: 23408424
    42. ERK1/2 signaling plays an important role in topoisomerase II poison-induced G2/M checkpoint activation. PMID: 23166842
    43. MRK is a novel RhoC effector that controls LPA-stimulated cell invasion at least in part by regulating myosin dynamics, ERK and p38 PMID: 23319595
    44. osteosarcoma patients whose tumors expressed pERK1 had a poorer clinical outcome than those whose tumors did not. PMID: 22935974
    45. Results showed significantly higher levels of ERK1 protein in smokers vs. non-smokers. Analysis revealed a significant relation among the number of cigarettes smoked daily, the Fagerstrom Test for Nicotine Dependence score and the mRNA expression of ERK1. PMID: 21070506
    46. constitutive activation of the ERK1 pathway in HER2/ERBB2-transformed cells prevents EGF deprivation-induced FLIPL upregulation and TRAIL resistance. PMID: 22722337
    47. Lead specifically induces dysregulation of iron response element (IRP)1 protein by activating the ERK1/2 signaling pathway, indicating a novel role for IRP1 and the ERK/MAPK pathway in vascular endothelial functions. PMID: 22502979
    48. CXCL12/CXCR4 protein signaling axis induces sonic hedgehog expression in pancreatic cancer cells via extracellular regulated kinase- and Akt kinase-mediated activation of nuclear factor kappaB PMID: 22995914
    49. Aortic endothelial cells stimulated with HLA class I antibodies did not promote any detectable change in intracellular Ca(2+) concentration but instead induced MLC phosphorylation and stress fiber assembly. PMID: 22914643
    50. The inhibition of Id-1 expression by MK615 is mediated via ERK1/2 activation. PMID: 22076920

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Membrane, caveola. Cell junction, focal adhesion.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6877

    OMIM: 601795

    KEGG: hsa:5595

    STRING: 9606.ENSP00000263025

    UniGene: Hs.861