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Recombinant Human N-glycosylase/DNA lyase (OGG1)

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  • 中文名稱：
    人OGG1重組蛋白
  • 貨號：
    CSB-EP016313HU
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    8 hydroxyguanine DNA glycosylase; 8 oxoguanine DNA glycosylase 1 ; 8 oxoguanine DNA glycosylase; AP lyase; DNA (apurinic or apyrimidinic site) lyase; DNA apurinic or apyrimidinic site lyase ; DNA lyase; DNA-(apurinic or apyrimidinic site) lyase; HMMH; HOGG 1; HOGG1; MMH; MUTM; N glycosylase; Ogg 1; Ogg1; OGG1_HUMAN; OGH 1; OGH1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    42.8kDa
  • 表達(dá)區(qū)域：
    1-345aa
  • 氨基酸序列
    MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
  • 基因功能參考文獻(xiàn)：
    1. study concludes that Ser326Cys polymorphism of the OGG1 gene may modulate the risk of colorectal cancer by increasing the level of oxidative DNA damage PMID: 29773766
    2. Results provide evidence that OGG1 rs1052133 is significantly associated with Wilms tumor susceptibility. PMID: 29937070
    3. TNF-alpha - Reactive Oxygen Species -driven enrichment of OGG1 at gene regulatory regions in the chromatinized DNA, which is a prerequisite to modulation of gene expression for prompt cellular responses to oxidant stress. PMID: 29940424
    4. OGG1 S326C polymorphism increases probability of AML relapse and may be useful as a prognostic factor for AML relapse risk. PMID: 29737460
    5. This study identifies patient's carrying the OGG1 Ser326Cys CC genotype are at greater risk for HIV associated low birth weight, and delivering prematurely. PMID: 29709520
    6. Of 39 subfertile men, 74.4% (29/39) had the hOGG1 Cys/Cys genotype. Patients in groups 1 and 2 with hOGG1 Cys/Cys genotype had significantly higher 8-OHdG content in sperm DNA, lower mitochondrial copy number in spermatozoa and lower TAC in seminal plasma than those with Ser/Ser or Ser/Cys genotype. PMID: 29582449
    7. CDK4/6 inhibitors also lead to accumulation of DNA damage by repressing PARP1 in oxidatively stressed cells. Thus, CDK4/6 inhibitors sensitize G1-arrested cells to anticancer drugs, since these cells require PARP1-OGG1 functional interaction for cell survival PMID: 29306194
    8. OGG1-2a, a critical enzyme for 8-OH-dG repair, is a direct target of miR-200a and its expression levels significantly decrease in aged keratinocytes. PMID: 29765508
    9. The level of OGG1 expression was significantly reduced in bipolar patients compared to healthy individuals, whereas the two groups exhibited similar levels of NEIL1 expression. PMID: 29626765
    10. Results identified a significant relationship between SNP rs13181 of OGG1 and an increased risk of endometrial cancer development. PMID: 30194171
    11. The OGG1 gene presents downregulation in the more advanced stages of colorectal cancer PMID: 29257843
    12. OGG1 gene Ser326Cys polymorphism was associated with age-related cataract risk (meta-analysis and review). PMID: 28631182
    13. Miners with the G/G genotype of the OGG1 (rs1052133) gene had an increased frequency of binucleated lymphocytes with micronuclei compared to the C/C genotype carriers. In addition, three-locus model of gene-gene interactions OGG1 (rs1052133) * ADPRT (rs1136410) * XRCC4 (rs6869366) was associated with high genotoxic risk in coal miners. PMID: 28992182
    14. Human Ogg1 removes 7,8-dihydro-8-oxoguanine (OG) paired with cytosine (C) but is inactive toward the OG:adenine (A) pair. In contrast, Geobacillus stearothermophilus Formamidopyrimidine-DNA Glycosylase removes OG from OG:C pairs and also exhibits appreciable (although diminished) activity toward OG:A pairs. PMID: 29320630
    15. For several turns of the helix, Authors observe that solution accessible 8-oxoGs are sites of activity for hOGG1. At the dyad axis, however, hOGG1 activity is suppressed, even at lesions predicted to be solution accessible by hydroxyl radical footprinting (HRF). PMID: 29341606
    16. The present meta-analysis concluded that the G allele was associated with an increased risk of cancer. It suggested that the hOGG1 polymorphism may be a candidate marker of cancer. PMID: 28415770
    17. hOGG1 mRNA is downregulated in non-small cell lung cancer tissues. Methylated +322-327 CpG site in the hOGG1 5'-UTR is associated with reduced expression of hOGG1 by decreasing the recruitment of Sp1 to the 5'-UTR of hOGG1 in A549 cells. PMID: 28586012
    18. uPA protection is independent of its catalytic activity, as the amino terminal fragment of uPA showed similar protection. A key enzyme for repairing oxidative DNA damage is the p53 target hOGG1. We found a significant increase of hOGG1 after pretreatment of HACM with uPA. Knockdown of hOGG1 completely abrogated the protective effect of uPA PMID: 28643198
    19. Study showed there was no association between hOGG1 rs1052133 and gastric cancer. [review] PMID: 28415729
    20. Ser326Cys variant effect on structural and functional properties of hOGG1 gene. PMID: 27822728
    21. SNP rs125701 in hOGG1 promoter was associated with the elevated gastric cancer risk. PMID: 27603140
    22. Provide strong evidence of an association between peripheral blood mononuclear cell hOGG1 methylation and the risk of non-small cell lung carcinoma in a Chinese population. PMID: 28039450
    23. HOGG1 Ser326Cys, APE1 Asp148Glu and XRCC1 Arg399Gln polymorphisms are correlated with the risk and clinicopathological features of PACG. PMID: 28396513
    24. The Gene expression levels of OGG1 was robustly elevated in oligodendrocytes laser captured from BA10 and amygdala white matter of Major Depressive Disorder. PMID: 28034960
    25. The findings showed that hOGG1-Ser326Cys Cys allele is statistically important and relevant with respect to the development of oral squamous cancer. PMID: 28448977
    26. Overexpressed beta-OGG1 has the same role as alpha-OGG1 in protecting bronchial epithelial cells from apoptosis and mitochondrial dysfunction. Additionally, knocking down OGG1 enhanced oxidative damage-induced apoptosis and mitochondrial dysfunction. The antiapoptotic function of beta-OGG1 involved the JNK signaling pathway. PMID: 29227732
    27. The rs2304277 variant in the OGG1 glycosidase gene associated to a significant OGG1 transcriptional down regulation independently of the BRCA mutational status and this variant may exert a synergistic effect together with BRCA1 or BRCA2 mutations in ovarian cancer PMID: 27015555
    28. OGG1 can initiate BER at positions off the dyad axis and that this activity is facilitated by spontaneous and transient unwrapping of DNA from the histones. Local nuances in the nucleosome environment and histone-DNA interactions can impact glycosylase activity. PMID: 28892740
    29. 8-oxoguanine DNA glycosylase-1 (OGG1) is the essential protein involved in oxidative stress-induced DNA demethylation. PMID: 27251462
    30. The allele combination of CGC from hOGG1, ITGA2 and XPD polymorphisms was significantly associated with increased odds of nasopharyngeal carcinoma. PMID: 29121049
    31. Increased oxidative DNA damage in primary open angle glaucoma may be attributed to decreased expression of DNA repair enzymes, OGG1 and PARP1, of the base excision repair pathway. PMID: 28129013
    32. Results show that some polymorphic variants in XRCC1 and OGG1 are associated with increased DNA damage in Alzheimer's Disease. PMID: 27011006
    33. The excision of the 8-oxoguanine base with DeltaG# = 16.1 kcal/mol proceeded via substitution of the C1-N9 N-glycosidic bond with an H-N9 bond where the negative charge on the oxoG base and the positive charge on the ribose were compensated in a concerted manner by NH3+(Lys249) and CO2-(Asp268), respectively. PMID: 28334993
    34. The interaction of OGG1 and XRCC1 DNA repair polymorphisms and arsenic exposure enhances telomeric DNA damage. PMID: 27084675
    35. These results together, points to a new paradigm about the role of DNA damage and repair by OGG1 in aging and age-associated disease processes. PMID: 27343030
    36. Enforced expression of hOGG1 and hAPE significantly protected thalamic neurons and motor neurons from retrograde apoptosis induced by target deprivation and axotomy. PMID: 27364693
    37. DNA repair protein OGG1 bound to its substrate is coupled to DNA occupancy of NF-kappaB and functions in epigenetic regulation of gene expression. PMID: 27756845
    38. Study shows increased level of 8-oxoG and decreased OGG1 protein in the serum of patients with Alzheimer's disease, suggesting that oxidative damage to DNA and a decreased DNA glycosylase are involved in the etiopathology of Alzheimer's disease PMID: 27538622
    39. APE1 Accelerates turnover of OGG1 by preventing retrograde binding to the abasic-site product. PMID: 28345889
    40. find that the energetic nature of the observed binding free energies of human 8-oxoguanine DNA glycosylase (hOGG1) and human uracil DNA glycosylase (hUNG) for undamaged DNA are derived from different sources. Although hOGG1 uses primarily nonelectrostatic binding interactions with nonspecific DNA, hUNG uses a salt-dependent electrostatic binding mode PMID: 27571472
    41. The Cys/Cys genotype of hOGG1 correlated strongly with the risk of developing ischemic cardiomyopathy. PMID: 27316582
    42. Polymorphisms of human 8-oxoguanine DNA glycosylase 1 and 8-hydroxydeoxyguanosine increase susceptibility to arsenic methylation capacity-related urothelial carcinoma. PMID: 26359225
    43. Polymorphisms in OGG1 gene is associated with gastrointestinal stromal tumours. PMID: 27460091
    44. Inherited OGG1 single nucleotide polymorphisms in Base-excision repair pathway are important determinants of oropharyngeal squamous cell carcinoma and predictors of patient outcomes. PMID: 27372710
    45. OGG1 and APE1 polymorphisms are associated with stage- and sex-specific risk of colorectal carcinoma in the Taiwanese population. PMID: 27022219
    46. The data of this study showed that OGG1 Ser326Cys and XRCC1 Arg399Gln gene polymorphisms had impacts on the development of stroke. PMID: 26936466
    47. remodeling by acetylation and dimethylation of lysine-14 and -4 residues of histone H3. In addition, OGG1 acts as a STAT1 coactivator and has transcriptional activity in the presence of endotoxin. The data presented here identifies a novel mechanism, and may provide new therapeutic strategies for the treatment of endotoxin-mediated inflammatory diseases. PMID: 26496208
    48. The frequency of OGG1 Ser326Cys polymorphisms was not significantly altered in Chinese patients with senile cataracts. PMID: 26884880
    49. This preliminary study suggests the possibility of different responses in DNA damage to the physical exercise training, considering the hOGG1 Ser326Cys polymorphism. PMID: 26592168
    50. OGG1 Ser/Cys and Ser/Cys + Cys/Cys genotypes had higher multiple sclerosis risk. PMID: 26562193

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  • 相關(guān)疾?。?/div>
    Renal cell carcinoma (RCC)
  • 亞細(xì)胞定位：
    Nucleus, nucleoplasm. Nucleus speckle. Nucleus matrix. Note=Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.; [Isoform 1A]: Nucleus.; [Isoform 2A]: Mitochondrion.
  • 蛋白家族：
    Type-1 OGG1 family
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 8125

    OMIM: 144700

    KEGG: hsa:4968

    UniGene: Hs.380271