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  4. Recombinant Human Neutrophil cytosol factor 2 (NCF2)

Recombinant Human Neutrophil cytosol factor 2 (NCF2)

  • 中文名稱：
    人NCF2重組蛋白
  • 貨號：
    CSB-YP015528HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人NCF2重組蛋白
  • 貨號：
    CSB-EP015528HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人NCF2重組蛋白
  • 貨號：
    CSB-EP015528HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人NCF2重組蛋白
  • 貨號：
    CSB-BP015528HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人NCF2重組蛋白
  • 貨號：
    CSB-MP015528HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    67 kDa neutrophil oxidase factor; Chronic granulomatous disease autosomal 2; FLJ93058; NADPH oxidase activator 2; NCF-2; Ncf2; NCF2_HUMAN; Neutrophil cytosol factor 2; Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); Neutrophil NADPH oxidase factor 2; NOXA2; P67 PHOX; p67-phox; p67phox
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-526
  • 氨基酸序列
    MSLVEAISLW NEGVLAADKK DWKGALDAFS AVQDPHSRIC FNIGCMYTIL KNMTEAEKAF TRSINRDKHL AVAYFQRGML YYQTEKYDLA IKDLKEALIQ LRGNQLIDYK ILGLQFKLFA CEVLYNIAFM YAKKEEWKKA EEQLALATSM KSEPRHSKID KAMECVWKQK LYEPVVIPVG KLFRPNERQV AQLAKKDYLG KATVVASVVD QDSFSGFAPL QPQAAEPPPR PKTPEIFRAL EGEAHRVLFG FVPETKEELQ VMPGNIVFVL KKGNDNWATV MFNGQKGLVP CNYLEPVELR IHPQQQPQEE SSPQSDIPAP PSSKAPGRPQ LSPGQKQKEE PKEVKLSVPM PYTLKVHYKY TVVMKTQPGL PYSQVRDMVS KKLELRLEHT KLSYRPRDSN ELVPLSEDSM KDAWGQVKNY CLTLWCENTV GDQGFPDEPK ESEKADANNQ TTEPQLKKGS QVEALFSYEA TQPEDLEFQE GDIILVLSKV NEEWLEGECK GKVGIFPKVF VEDCATTDLE STRREV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 基因功能參考文獻：
    1. We analyzed the clinical and laboratory findings of CGD with mutations in the NCF2 gene from amongst our cohort of CGD patients. A homozygous mutation (c.835_836delAC, p.T279fsX294), a deletion in NCF2 gene was found in two cases. In the third case, two heterozygous mutations were detected, IVS13-2A>T on one allele and c.1099C>T (p.) on the other allele. PMID: 28035544
    2. All investigated patients presented the same mutation (c.257 + 2T > C) in NCF2 gene. We show that this mutation is responsible for a drastic decrease of p67phox mRNA and leads to the skipping of exon 3 detected in the low amount of residual mRNA. PMID: 27220316
    3. Phosphoinositol 3-phosphate regulates reactive oxygen species production by maintaining p40phox and p67phox at the phagosomal membrane. PMID: 28096301
    4. TLR4- and TLR2-induced IRAK-ERK pathway cross-talks with p67phox-Nox-2 for reactive oxygen species generation, thus regulating IL-1beta transcription and processing in monocytic cells. PMID: 26320741
    5. Skeletal muscle protein expression of the NADPH oxidase subunits p22(phox), p47(phox), and p67(phox) was increased in obese relative to lean subjects, where p22(phox) and p67(phox) expression was attenuated by exercise training in obese subjects. PMID: 27765769
    6. A novel homozygous mutation in NCF2. PMID: 26272171
    7. results reveal an essential role for the Cys-Gly-Cys triad in Nox2 in binding p67(phox), seconded by an additional binding region, comprising residues C terminal to Cys-Gly-Cys. The 2 regions interact with distinct partner sites in p67(phox). PMID: 26160850
    8. This model assigns a central role to Arg-395 in the structure and stability of the quaternary NCF2/NCF4/VAV1/RAC1 NADPH oxidase complex. PMID: 25795782
    9. Data indicate that arachidonic acid induces the direct interaction of Rac-GTP-bound p67(phox) with the C-terminal cytosolic region of phagocyte NADPH oxidase Nox2. PMID: 25056956
    10. Four novel mutations in the NCF1, NCF2, and CYBB genees have been identified in chronic granulomatous disease patients in Morocco. PMID: 24596025
    11. Results not only establish allelic heterogeneity within NCF2 associated with SLE, but also emphasize the utility of multi-ethnic cohorts to identify predisposing variants explaining additional phenotypic variance of complex diseases like SLE. PMID: 24163247
    12. NCF2 in Asian populations shows a pattern of diversity characterized by a differentiated haplotype structure. PMID: 23821607
    13. Results provide insight into the redox-sensitive signaling mechanism that mediates cell survival involving p53 and its novel target NCF2/p67phox. PMID: 23187810
    14. Case Report: report defects in NCF-2, teh gene encoding p67-phox, in four cases of chronic granulomatous disease. PMID: 23264412
    15. Eight novel mutations in CYBB and NCF2 genes were identified in patients with chronic granulomatous disease. PMID: 22562447
    16. This variant reduced binding of the NCF2 gene product p67(phox) to RAC2. This study found a novel genetic association of RAC2 with Crohn's disease (CD) and replicated the previously reported association of NCF4 with ileal CD. PMID: 21900546
    17. NCF2 is strongly associated with increased risk of childhood- and adult-onset systemic lupus erythematosus through a single nonsynonymous coding mutation (H389Q) in exon 12. PMID: 22203994
    18. p67(phox) has a critical role to support for reactive oxygen species production on the level of individual phagosomes. PMID: 21954286
    19. Association analysis identified five SLE susceptibility genes reaching genome-wide levels of significance : NCF2 ,IKZF1 ,IRF8 ,IFIH1 , and TYK2 PMID: 22046141
    20. The genetic variation in the NCF2 gene was found to associate with SLE in US and European populations PMID: 20842512
    21. High NCF2 expression in the cytoplasm is associated with uterine cervix carcinogenesis. PMID: 21119665
    22. the extended activation domain of p67(phox) (amino acids 190-210) containing the D(Y/F)LGK motif plays an essential role in oxidase activation probably by interacting with gp91(phox). PMID: 20679349
    23. mutations in CYBB, NCF1, CYBA or NCF2 may play a role in chronic granulomatous disease PMID: 18546332
    24. There is an increased expression of NADPH oxidase p47(-PHOX) and p67(-PHOX) factor in idiopathic pulmonary fibrosis patients. PMID: 17651608
    25. All mutations and some polymorphisms identified in the NCF2 gene in the autosomal forms of chronic granulomatous disease are listed. Review. PMID: 20167518
    26. Here we show that p67(phox)adopts an elongated conformation when it exists not only as a monomer but also as the heterotrimer PMID: 20375610
    27. These findings identify the activation of PKC delta and NADPH oxidase as crucial steps in retinoic acid-induced neuroblastoma cell differentiation. PMID: 20074641
    28. Alu-induced deletion of the TPR4 domain of p67-phox leads to loss of function and accelerated degradation of the protein. PMID: 19953534
    29. In a cell-free system, covalent binding between C-terminal-truncated p67phox and rac in the correct fusion order produces a more stable complex than the individual components and significantly influences the duration of fusion-produced oxidase activation. PMID: 11705402
    30. detailed study of the protein-protein interactions that occur in the p40-p47-p67(phox) complex of the resting oxidase PMID: 11796733
    31. p22(phox), gp91(phox), p47(phox), p67(phox), and p40(phox) existed as a functional complex in the cytoskeletal fraction. PMID: 11893732
    32. Val204 in p67(phox), previously shown to be required for NADPH oxidase activity under cell-free conditions, was found to be essential for superoxide production by intact COS-phox cells. PMID: 11929750
    33. effect of cPLA2 on its translocation PMID: 12101222
    34. NAD(P)H oxidase subunits p47(phox) and p67(phox) are expressed in platelets; and NAD(P)H oxidase-dependent platelet superoxide anion release increases platelet recruitment. PMID: 12130503
    35. p67phox and p47phox have roles in regulating a change of conformation in cytochrome b558, which initiates the electron transfer in NADPH oxidase activation PMID: 12719414
    36. NOXO1, p47phox, and p67phox regulate Nox3 PMID: 15181005
    37. NAD(P)H oxidase activity is associated with increased protein levels of p22phox, p47phox, and p67phox, and increased p22phox and nox2 (gp91phox) mRNA expression. PMID: 15256399
    38. Increased expression and activity of NAD(P)H oxidase subunits and xanthine oxidase, in part mediated through angiotensin II and PKC-dependent pathways, are important mechanisms underlying increased oxidative stress in human coronary artery disease PMID: 16293794
    39. Here we show that the p47(phox)-p67(phox) interaction is disrupted not only by deletion of the PRR but also by substitution for basic residues in the extra-PRR (K383E/K385E). PMID: 16297854
    40. Expression of p67(phox) is regulated through mechanisms that include modulation of transcription and translation. PMID: 16310324
    41. These results indicate that Hcy (homocysteine)-stimulated superoxide anion production in monocytes is regulated through PKC-dependent phosphorylation of p47phox and p67phox subunits of NADPH oxidase. PMID: 16626305
    42. NADPH oxidase assembly from p67phox was studies at the single-cell level. PMID: 16987007
    43. chemoattractant-stimulated superoxide production can be amplified by a positive feedback loop in which p67(phox) targets Vav1-mediated Rac activation PMID: 17060455
    44. These data clearly identify PLAGL2 as a novel regulator of NCF2/p67phox gene expression as well as NADPH oxidase activity and contribute to a greater understanding of the transcriptional regulation of NCF2. PMID: 17462995
    45. a novel single nucleotide polymorphism in the promoter region PMID: 17712795
    46. Single nucleotide polymorphism leads to alternative splicing without altering gene expression or respiratory burst activity. PMID: 17910042
    47. p40(phox) translocates p67(phox) to the region of the cytochrome and subsequently switches the oxidase to an activated state dependent upon PtdIns(3)P and SH3 domain engagement. PMID: 18029359
    48. As(2)O(3) induced phosphorylation and membrane translocation of the NADPH oxidase subunit p47(phox) and it also increased translocation of Rac1 and p67(phox). PMID: 18424721
    49. autosomal recessive CGD due to NCF-2 gene mutations, and a novel homozygous and hypomorphic NCF-2 gene mutation was found. PMID: 18625437
    50. p67(phox)-SH3(N) specifically functions in gp91(phox)/Nox2 activation probably via facilitating oxidase assembly. PMID: 19116138

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  • 相關疾病：
    Granulomatous disease, chronic, cytochrome-b-positive 2, autosomal recessive (CGD2)
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    NCF2/NOXA1 family
  • 數據庫鏈接：

    HGNC: 7661

    OMIM: 233710

    KEGG: hsa:4688

    STRING: 9606.ENSP00000356505

    UniGene: Hs.587558