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  4. Recombinant Human Non-receptor tyrosine-protein kinase TYK2 (TYK2), partial

Recombinant Human Non-receptor tyrosine-protein kinase TYK2 (TYK2), partial

In Stock
  • 中文名稱：
    人TYK2重組蛋白
  • 貨號(hào)：
    CSB-BP025391HU5
  • 規(guī)格：
    ￥3168
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Partial
  • 來(lái)源：
    Baculovirus
  • 分子量：
    34.4 kDa
  • 表達(dá)區(qū)域：
    589-875aa
  • 氨基酸序列
    ITQLSHLGQGTRTNVYEGRLRVEGSGDPEEGKMDDEDPLVPGRDRGQELRVVLKVLDPSHHDIALAFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLRRERGHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSPFIKLSDPGVGLGALSREERVERIPWLAPECLPGGANSLSTAMDKWGFGATLLEICFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQLATLTSQCLTYEPTQRPSFRTILRDLTRLQPHNLA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Non-receptor kinase involved in various processes including cell growth, development, cell migration, innate and adaptive immunity. Plays both structural and catalytic roles in numerous cytokines and interferons signaling. Associates with cytokine and growth factor receptors and activate STAT family members including STAT1, STAT3, STAT4 or STAT6. The heterodimeric cytokine receptor complexes are composed of a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1) which serves as the signal transducing chain harboring STAT docking sites once phosphorylated by TYK2, and a second receptor chain associated either with JAK1 or JAK2. In turn, recruited STATs are phosphorylated, form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes. Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling.
  • 基因功能參考文獻(xiàn)：
    1. New ELAVL1-TYK2 fusion gene is oncogenic in acute myeloid leukemia. PMID: 27189703
    2. TYK2 gene variants do not seem to play an important role in Chagas disease susceptibility and/or chronic Chagas cardiomyopathy PMID: 29304122
    3. TYK2 polymorphism is not associated with Dermatomyositis /Polymyositis in the Chinese Han population PMID: 28846454
    4. TYK2 activating mutants and fusion proteins were detected in patients diagnosed with leukaemic diseases suggesting that TYK2 is a potent oncogene. PMID: 26631911
    5. TYK2 genetic variants are differentially associated with common autoimmune diseases PMID: 27807284
    6. High TYK2 expression is associated with B-cell lymphoma. PMID: 28716895
    7. These data indicate that germline activating TYK2 mutations predispose to the development of ALL. PMID: 27733777
    8. TYK2 rs280519 genotype was significantly related to severe necroinflammatory activity (NIA) grade of chronic hepatitis C patients. PMID: 28704535
    9. TYK2 is associated with systemic sclerosis susceptibility and reinforce the relevance of the IL-12 pathway in SSc pathophysiology. PMID: 26338038
    10. Clinical genomic analysis of the current series of NF1-MPNST cases found that TYK2 is a new gene mutated in MPNST. PMID: 27875628
    11. Results demonstrates that the TYK2 rs2304256 and rs12720356 polymorphisms are associated with susceptibility to rheumatic diseases, rs2304256 polymorphism is associated with systemic lupus erythematous (SLE) in Caucasians, and rs280519 polymorphism is associated with SLE in Caucasians and Asians. [meta-analysis] PMID: 26980740
    12. demonstration of the single marker association of tyrosine kinase-2 polymorphisms with ulcerative colitis and Crohn's disease in Turkish population. PMID: 25744728
    13. The TYK2 promoter variant is associated with an overall risk for diabetes. PMID: 26288847
    14. Genetic associations and gene-gene interactions of IRF5 and TYK2 were significantly detected in Han Chinese with systemic lupus erythematosus PMID: 26294277
    15. The majority of disease-associated mutations in JAKs map to a pseudokinase domain (JH2), demonstrating its central regulatory function. PMID: 26359499
    16. the role of TYK2 in the pathogenesis of rheumatoid arthritis PMID: 25849893
    17. present findings suggest that TYK2 regulates apoptotic and proinflammatory pathways in pancreatic beta-cells via modulation of IFNalpha signaling, subsequent increase in MHC class I protein, and modulation of chemokines such as CXCL10. PMID: 26239055
    18. Tyrosine Kinase 2-mediated Signal Transduction in T Lymphocytes Is Blocked by Pharmacological Stabilization of Its Pseudokinase Domain. PMID: 25762719
    19. Data indicate a chimeric fusion involving nucleophosmin NPM1 (5q35) and TYK2 kinase (19p13) that encodes an NPM1-TYK2 protein in cutaneous CD30 antigen-positve lymphoproliferative disorders. PMID: 25349176
    20. Immune cell dysfunction may contribute to enhancement and maintenance of immune inflammatory and autoimmune responses by production of pathogenic cytokines and immune complexes. The role of Tyk2 in innate and adaptive immune cells is discussed. Review. PMID: 24654603
    21. In cellular assays, SAR-20347 dose dependently (1 nM-10 muM) inhibited JAK1- and/or TYK2-dependent signaling from the IL-12/IL-23, IL-22, and IFN-alpha receptors. PMID: 25156366
    22. TYK2 as a novel associated locus for idiopathic inflammatory myopathies. PMID: 24812289
    23. Here, we report the 2.8-A structure of the two-domain pseudokinase-kinase module from the JAK family member TYK2 in its autoinhibited form. PMID: 24843152
    24. The crystal structure of a receptor-binding fragment of human TYK2, encompassing the FERM and SH2 domains, in complex with peptide motif from the interferon-alpha receptor chain 1 (IFNAR1) is presented. PMID: 24704786
    25. Our results indicate that the biological processes that require TYK2 catalytic function in humans are restricted to the IL-12 and IL-23 pathways. PMID: 23894201
    26. TYK2 rs34536443 polymorphism is associated with a decreased susceptibility to endometriosis-related infertility. PMID: 23000200
    27. Two rare autoimmune disease-associated Tyk2 variants are catalytically impaired but signaling competent. PMID: 23359498
    28. The rs6445975 polymorphism of phox homology domain containing serine/threonine kinase and the rs2304256 polymorphism of tyrosine kinase 2 are associated with the development of systemic lupus erythematosus in Europeans PMID: 22592861
    29. The most associated variant in primary biliary cirrhosis was in chromosome 19, a low-frequency nonsynonymous single nucleotide polymorphism in TYK2. PMID: 22961000
    30. In our pilot study, we discovered significant changes in methylation patterns of genes IL-7, IL-13, IL-17C and TYK2 between henodialysis patients and healthy subjects PMID: 22506826
    31. This study demonistrated that Rs55762744 is a rare variant of modest effect on multiple sclerosis risk affecting a subset of patients. PMID: 22744673
    32. Studied the doubly tagged full-length construct, H6-FL-TYK-2-FLAG. In the presence of ATP and a peptide substrate, H6-FL-TYK-2-FLAG showed a marked lag in phosphopeptide product formation. TYK-2 KD showed no such lag. PMID: 22486643
    33. data highlight the role of TYK2 downregulation in breast cancer cell de-differentitation and initiation of regional metastasis PMID: 22116632
    34. Association analysis identified five SLE susceptibility genes reaching genome-wide levels of significance : NCF2 ,IKZF1 ,IRF8 ,IFIH1 , and TYK2 PMID: 22046141
    35. infection of airway epithelial cells with hMPV decreased cellular level of Janus tyrosine kinase (Jak1) and tyrosine kinase 2 (Tyk2) PMID: 21949722
    36. This meta-analysis demonstrates that autoimmune and inflammatory diseases is associated with TYK2 gene rs34536443 and rs2304256 polymorphisms, but not rs280523, rs280519, rs12720270 and rs12720356. PMID: 21140222
    37. The consequent effect of SOCS1 inhibition of Tyk2 not only results in a reduced IFN response because of inhibition of Tyk2 kinase-mediated STAT signaling but also negatively impacts IFNAR1 surface expression, which is stabilized by Tyk2. PMID: 21757742
    38. Data suggest that TYK2 polymorphisms were not associated with systemic lupus erythematosus in Hong Kong Chinese, but that rs2304256 and rs12720270 may be associated with photosensitivity and discoid rash. PMID: 21196586
    39. The rs34536443 variant effect on multiple sclerosis susceptibility might be mediated by deviating T lymphocyte differentiation toward a Th2 phenotype. PMID: 21354972
    40. Tyk2 deficiency and clinical manifestations of hyper IgE syndromes (Review) PMID: 21178271
    41. Siva-1 forms a functional complex with Tyk2 and participates in the transduction of signals that inhibit B lymphocyte growth. PMID: 20727854
    42. Here, the authors report the crystal structures of TYK2, a first in class structure, and JAK3 in complex with PAN-JAK inhibitors CP-690550 and CMP-6, both of which bind in the ATP-binding cavities of both JAK isozymes. PMID: 20478313
    43. Single-nucleotide polymorphism in TYK2 gene is confirmed to be associated with multiple sclerosis. PMID: 19888296
    44. rs2304256 associated with increased risk of discoid lupus erythematosus PMID: 19758313
    45. Studies indicate that the highest T1D association was at marker rs2304256 (odds ratio (OR) = 0.86; 95%CI = 0.82-0.90) in the TYK2 gene 19p13.2See TYK2 gene at chromosome 19p13.2. PMID: 19966805
    46. Data show that Tyk2 tyrosine kinase is essential for stable cell surface expression of IFNAR1. PMID: 12554654
    47. evidence that the small GTPases RhoA and Rac1, but not Cdc42, are directly associated with Tyk2 and PI3-K in an uPA/uPAR-dependent fashion and are necessary to mediate the uPA/uPAR-directed migration via the Tyk2/PI3-K signalling complex in human VSMC PMID: 12719789
    48. Catalytically active Tyk2 is necessary for Janus kinase 2 phosphorylation and association with the platelet-activating factor receptor. PMID: 14500680
    49. In the IFN signaling pathway leading to STAT activation, both JAK1 and TYK2 are essential, whereas NF-kappaB activation requires only TYK2. PMID: 15883164
    50. mutations in the JAK1 and Tyk2 genes may be identified as initial molecular defects in human cancers and autoimmune diseases PMID: 16239216

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  • 相關(guān)疾病：
    Immunodeficiency 35 (IMD35)
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, JAK subfamily
  • 組織特異性：
    Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 12440

    OMIM: 176941

    KEGG: hsa:7297

    STRING: 9606.ENSP00000264818

    UniGene: Hs.75516