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  4. Recombinant Human Nuclear receptor coactivator 3 (NCOA3), partial

Recombinant Human Nuclear receptor coactivator 3 (NCOA3), partial

  • 中文名稱：
    人NCOA3重組蛋白
  • 貨號：
    CSB-YP015541HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人NCOA3重組蛋白
  • 貨號：
    CSB-EP015541HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人NCOA3重組蛋白
  • 貨號：
    CSB-EP015541HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人NCOA3重組蛋白
  • 貨號：
    CSB-BP015541HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人NCOA3重組蛋白
  • 貨號：
    CSB-MP015541HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ACTR; AIB 1; AIB-1; AIB1; Amplified in breast cancer 1; Amplified in breast cancer 1 protein; bHLHe42; CAGH 16; CAGH16; CBP interacting protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG 26; CTG26; KAT13B / AIB1; MGC141848; NCOA 3; NCoA-3; Ncoa3; NCOA3_HUMAN; Nuclear receptor coactivator 3; pCIP; RAC 3; RAC-3; RAC3; Receptor associated coactivator 3; Receptor-associated coactivator 3; SRC 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM 1; TRAM-1; TRAM1
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
  • 基因功能參考文獻：
    1. AKT is involved in regulating AIB1 recruitment to the PS2 promoter region in the presence of IGF-1. AIB1 overexpression in breast cancer cells increases AKT activity, as well as enhances PI3K/AKT-mediated cell cycle S phase entry. PMID: 29808803
    2. Study shows that oxidative stress plays a crucial role in controlling steroid receptor coactivator 3 expression, which influences the migration and tube formation abilities of endothelial cells through the PI3K/Akt/mTOR signaling pathways. PMID: 28826365
    3. stable knockdown of FOXO3, NCOA3, and TCF7L2 restored growth in low glucose but reduced MEK/MAPK phosphorylation, reduced anchorage-independent growth, and modulated expressions of GLUT1 and Ras pathway related proteins. PMID: 29301589
    4. The Authors reveal a gender difference in the prognostic value of concomitant AIB1 and HER2 copy number gain (CNG) in glioma patients which were barely noticed before. These observations indicated that genetic alterations synergistic with essential respects of sex determination influence glioma biology and patients outcomes. PMID: 30153912
    5. NCOA3 maintained the molecular signature of chondrocytes dedifferentiating in vitro or exposed to IL-1 beta and NCOA3 lose promotes posttraumatic OA at least partially by enhancing NF-kappaB activation. PMID: 30261507
    6. Data suggest that NEAT1, SRC3, and IGF1R are highly expressed in prostate cancer cells; NEAT1 appears to interact with SRC3 and promote cell proliferation via up-regulation of SRC3/IGF1R/AKT signaling pathway. (NEAT1 = nuclear paraspeckle assembly transcript-1; SRC3 = steroid receptor coactivator protein-3; IGF1R = insulin-like growth factor 1 receptor) PMID: 29225160
    7. findings suggest that the Warburg pathway enzyme PFKFB4 acts as a molecular fulcrum that couples sugar metabolism to transcriptional activation by stimulating SRC-3 to promote aggressive metastatic tumours PMID: 29615789
    8. Findings demonstrate how SRC3 and Cx43 regulation between BMSCs and myeloma cells mediate cell growth and disease progression. PMID: 29075794
    9. RAC3 bound tightly to the ARE enhancer region of the HO-1 promoter via Nrf2 binding. PMID: 22370642
    10. Hypoxia inhibits the RAC3 gene expression leading to the autophagy process, allowing tumor cells to survive until angiogenesis occurs PMID: 22957814
    11. RAC3 is an inhibitor of senescence whose downregulation in aged individuals could be probably a tumor suppressor mechanism. PMID: 26469953
    12. The model had 92% sensitivity and 92% specificity. We obtained similar results in the independent validation cohort. AIB1 and EIF5A2 show promise for the noninvasive detection of bladder cancer. The model based on AIB1, EIF5A2, and NMP22 outperformed each of the three individual biomarkers for detecting Bladder cancer. PMID: 27203388
    13. our study implicates that AIB1 is a molecular target of sorafenib and downregulation of AIB1 contributes to the anti-tumor effects of sorafenib PMID: 27105488
    14. our studies illustrate that SRC-3 overexpression is clinically and functionally relevant to the progression of human ESCC. PMID: 27781415
    15. Estrogen receptor recruits steroid receptor coactivator-3 primary coactivator and secondary coactivators, p300/CBP and CARM1 to regulate genetic transcription. PMID: 28844863
    16. loss of miR-17 and miR-20b enhanced breast cancer resistance to taxol by upregulating NCOA3 levels. PMID: 27831559
    17. Data (including data from studies of hydrogen-deuterium exchange coupled to mass spectrometry in presence of denaturants) suggest that, for peptide fragments of human ACTR and mouse Crebbp representing disordered interaction domains, exchange rates are changed dramatically by high concentrations of denaturants guanidinium chloride or urea. (ACTR = activator of thyroid and retinoid receptor; Crebbp = CREB binding protein) PMID: 28675294
    18. Data suggest that steroid receptor coactivators (NCOA1, NCOA2, NCOA3) are over-expressed in a number of hormone-dependent cancers where they promote tumor growth, invasion, metastasis, and chemo-resistance; with their multiple roles in cancer, steroid receptor coactivators are promising targets for development of antineoplastic agents that can interfere with their function. [REVIEW] PMID: 28390937
    19. Here, we reported the novel finding that depletion of SRC-3 enhanced sensitivity of breast and lung cancer cells to HDAC inhibitors (SAHA and romidepsin). In contrast, overexpression of SRC-3 decreased SAHA-induced cancer cell apoptosis. Furthermore, we found that SRC-3 inhibitor bufalin increased cancer cell apoptosis induced by HDAC inhibitors. The combination of bufalin and SAHA was particular efficient in attenuating PMID: 27425252
    20. Altered expression of AIB1 may play a role in adenomyosis development and treatment outcome with levonorgestrel-releasing intrauterine system. PMID: 26040939
    21. The oncogenic transcription factor AIB1 has a novel role in the regulation of polyribosome recruitment and formation of the translational complex. Combinatorial therapies targeting IGF signaling and mRNA translation in AIB1 expressing breast cancers may have clinical benefit and warrants further investigation PMID: 26936396
    22. Total SRC3 is selectively found at enhancer regions. PMID: 26369632
    23. The transcriptional coactivator SRC-3. PMID: 26833126
    24. The aim of this study was to investigate the expression of AIB1 in human esophageal squamous cell carcinoma and its correlation with Ki67 expression. PMID: 26345978
    25. NCOA3 polymorphisms might influence the risk of dyslipidemia and serum lipid levels in Chinese Han population. PMID: 26449542
    26. miR-137 has a role in targeting p160 steroid receptor coactivators SRC1, SRC2, and SRC3 and inhibits cell proliferation PMID: 26066330
    27. AIB1 promotes gastric cancer cell proliferation, survival and invasiveness through modulating major signaling pathways such as ErbB and Wnt/beta-catenin pathways. Findings suggest that AIB1 plays an important role in the pathogenesis of gastric cancer. PMID: 25970779
    28. ER-alpha, SRC-3, and p300 form an active protein complex on estrogen response element-DNA. PMID: 25728767
    29. NCOA3 is subject to a cis-acting expression quantitative trait locus in articular cartilage, which correlates with the osteoarthritis association signal and with the OA-associated allele of the SNP rs116855380, which is located 10.3 kb upstream of NCOA3. PMID: 26211391
    30. we describe a TR-FRET assay that monitors the interaction of the estrogen receptor (ER) alpha ligand binding domain (labeled with a terbium chelate via a streptavidin-biotin interaction) with a sequence of coactivator protein SRC3 PMID: 25859975
    31. NCOA3 upregulates mucin 1 and 4 via transcriptional and post-translational changes in pancreatic cancer. PMID: 25531332
    32. Data suggest that over-stimulating the steroid seceptor coactivators SRC-1, SRC-2, and SRC-3 oncogenic program can be an effective strategy to kill cancer cells. PMID: 26267537
    33. AIB1 promotes colorectal cancer progression at least in part through enhancing Notch signaling. PMID: 25263446
    34. Our study clearly demonstrated differentiation-dependant expression of SRC-1 and SRC-3 in chondrosarcoma PMID: 24875297
    35. Results indicate a combination of decreased expression of ERs, AR and SRC-3 but not SRC-1 may be involved in the tumorigenesis of gliomas, ERalpha/SRC-3 axis may play central role in the regulation these tumors. PMID: 24680642
    36. AIB1, AIB1-delta4 and ANCO1 are important determinants of endocrine and growth factor responsiveness in breast cancer. PMID: 24678732
    37. verrucarin A is a potent and selective SMI that blocks SRC-3 function through an indirect mechanism. PMID: 24743578
    38. we found that MicroRNA-195 (miR-195) could negatively regulate protein levels of SRC-3 through targeting its 3'-untranslated region (3'-UTR) in hepatocellular carcinoma (HCC) cells PMID: 24740565
    39. ERK3 regulates endothelial cell migration, proliferation and tube formation by upregulating SRC-3/SP-1-mediated VEGFR2 expression. PMID: 24585635
    40. The above results clearly demonstrated a high frequency of overexpression of SRC-3 immunoreactivities in different bone cancers, indicating its potential roles in the prognosis and treatment of these cancers. PMID: 24134957
    41. Results propose that increased expression of AIB1, through the maintenance of breast cancer-initiating cell, facilitates formation of ductal carcinoma in situ, a necessary step before development of invasive disease PMID: 23851504
    42. Bufalin as a potent small-molecule inhibitor for SRC-3 and SRC-1. PMID: 24390736
    43. overexpression of SRC-3 promoted glycolysis in bladder cancer cells through HIF1alpha to facilitate tumorigenesis, which may be an intriguing drug target for bladder cancer therapy. PMID: 24584933
    44. Up-regulation of nuclear receptor coactivator amplified in breast cancer-1 in papillary thyroid carcinoma correlates with lymph node metastasis. PMID: 23606350
    45. Our results suggest that high AIB1 is a predictive marker of good response to tamoxifen treatment in postmenopausal women and a prognostic marker of decreased recurrence-free survival in systemically untreated patients. PMID: 23670096
    46. FoxG1 can function as a pro-apoptotic factor in part through suppression of AIB1 coactivator transcription complex formation, thereby reducing the expression of the AIB1 oncogene. PMID: 23660594
    47. NCOA3 is a selective co-activator of estrogen receptor alpha-mediated transactivation of PLAC1. PMID: 24304549
    48. Up-regulation of SRC-3 is associated with bladder cancer. PMID: 23886194
    49. AIB1 exerted its effect on epithelial mesenchymal transition through its interaction with ERalpha, which could directly bind to the ERalpha-binding site on the SNAI1 promoter. PMID: 23762395
    50. study confirm AIB1 to be a negative prognostic factor in an independent cohort of lymph-node-negative premenopausal breast cancer patients PMID: 23230135

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • 蛋白家族：
    SRC/p160 nuclear receptor coactivator family
  • 組織特異性：
    Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 7670

    OMIM: 601937

    KEGG: hsa:8202

    STRING: 9606.ENSP00000361066

    UniGene: Hs.592142