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  4. Recombinant Human Partitioning defective 3 homolog (PARD3), partial

Recombinant Human Partitioning defective 3 homolog (PARD3), partial

  • 中文名稱：
    人PARD3重組蛋白
  • 貨號(hào)：
    CSB-YP819481HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人PARD3重組蛋白
  • 貨號(hào)：
    CSB-EP819481HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人PARD3重組蛋白
  • 貨號(hào)：
    CSB-EP819481HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人PARD3重組蛋白
  • 貨號(hào)：
    CSB-BP819481HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人PARD3重組蛋白
  • 貨號(hào)：
    CSB-MP819481HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    agouti signaling protein; ASIP; Atypical PKC isotype specific interacting protein; Atypical PKC isotype-specific-interacting protein; Baz; bazooka; Coagulation factor II receptor-like 2; CTCL tumor antigen se2-5; F2rl2; PAR-3; par-3 family cell polarity regulator alpha; par-3 family cell polarity regulator; PAR3 alpha ; par3; PAR3-alpha; PARD-3; Pard3; PARD3_HUMAN; PARD3A; Partitioning defective 3 homolog; Partitioning-defective 3 homolog; Partitioning-defective protein 3; C. elegans; homolog of; SE2-5L16; SE2-5LT1; SE2-5T2; Thrombin receptor-like 2
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
  • 基因功能參考文獻(xiàn)：
    1. Studies indicate that the PAR3-PAR6-aPKC complex are important for the establishment of neuronal polarity [Review]. PMID: 29696344
    2. that this heterozygotic microdeletion showed no tissue specificity and led to defective expression of PARD3 PMID: 28623428
    3. The authors identified a tumor-suppressive property function of Par3 in human cervical cancer and the lost Par3 expression may be a marker of poor prognosis. PMID: 29953780
    4. We first demonstrate that Hook2 is essential for the polarized Golgi re-orientation towards the migration front. Depletion of Hook2 results in a decrease of PAR6alpha at the centrosome during cell migration, while overexpression of Hook2 in cells induced the formation of aggresomes with the recruitment of PAR6alpha, aPKC and PAR3 PMID: 27624926
    5. elevated expression of Par3 promotes PCa metastasis via KIBRA sequestration-mediated inactivation of the Hippo pathway to upregulate expression of pro-metastatic genes. PMID: 29017577
    6. reduced expression of PKCzeta/Pard3/Pard6 contributes to non-small-cell lung cancer epithelial-mesenchymal transition, invasion, and chemoresistance. PMID: 28652146
    7. PARD3 might play a tumor suppressor role in esophageal squamous cell carcinoma. PMID: 28526815
    8. Loss of Par3 promotes metastatic behavior in lung adenocarcinoma cells through 14-3-3zeta protein. PMID: 27588399
    9. Studies suggest that rare deleterious variants of PARD3 in the aPKC-binding region contribute to human cranial neural tube defect (NTD). PMID: 27925688
    10. These data highlight the importance of the carboxy-terminal motif of the E6 protein and downregulation of PAR3 in tumorigenic transformation of human cervical keratinocytes. PMID: 28440909
    11. Results suggest that Par3 expression is likely involved in ovarian cancer progression, especially in peritoneal metastasis, probably through modulating IL-6 /STAT3 signaling. PMID: 27855669
    12. reduced keratinocytes Par3 function fosters a permissive P-cadherin-dependent niche for melanocytes transformation, invasion, and metastasis. PMID: 28096290
    13. These results demonstrate the importance of Par3 and SNAIL in development of KSHV-induced B-cells cancers PMID: 27463802
    14. PAR-3 protein expression is frequently lost in primary esophageal squamous cell carcinoma and loss of the PAR-3 protein is associated with aggressive clinicopathological features. PMID: 28188749
    15. Taken together, these results suggest that the Par3 regulates invasion and metastasis in pancreatic cancers by controlling tight junction assembly via Tiam1. PMID: 26084985
    16. Knockdown of the polarity protein Par3 reversed the effects of Galpha13 knockdown on cell-cell adhesion and proteolytic invasion in three-dimensional collagen. PMID: 26589794
    17. Par-3 plays an important role in the modulation of intestinal barrier function by regulating delivery of occludin as well as suppression of MLC phosphorylation. PMID: 25820151
    18. Shp2 promotes metastasis of prostate cancer by attenuating the PAR3/PAR6/aPKC polarity protein complex and enhancing epithelial-to-mesenchymal transition PMID: 26050620
    19. study indicated a potential molecular basis for cell growth-promoting function of PARD3 by modulating the Hippo pathway signaling in response to cell contact and cell polarity signals PMID: 26116754
    20. PAR3-mutant proteins exhibit a relative reduction in the ability to mediate formation of tight junctions and actin-based protrusions and activate STAT3 at cell confluence. PMID: 25833829
    21. PAR3 and aPKC control the organization of the Golgi through CLASP2 phosphorylation. PMID: 25518939
    22. Data show that increased partitioning defective 3 (Par-3) expression is associated with distant metastasis and poor survival rates in patients with hepatocellular carcinoma (HCC). PMID: 23322019
    23. A cytoplasmic expression of PAR-3 is therefore implicated in worse clinical and pathological cancer features in clear cell renal cell carcinoma and could be useful to identify patients with high-risk tumors. PMID: 24856572
    24. The PAR polarity complex of PARD3, PARD6, and an atypical protein kinase C (aPKC) regulate several aspects of neuronal migration.[review] PMID: 24243103
    25. Our results suggest that PAR-3 has a role in the clinical aggressiveness of cell Renal Cell Carcinoma PMID: 24136590
    26. Data indicate that both tumor focality and Par3/Par6/atypical protein kinase C (APKC) expression were significantly associated with tumor recurrence. PMID: 21549621
    27. Suggest that loss of Par3 promotes metastatic behaviour of ErbB2-induced breast tumour epithelial cells by decreasing cell-cell cohesion. PMID: 23263278
    28. Par3 is identified as a regulator of signaling pathways relevant to invasive breast cancer. PMID: 23153534
    29. A VE-cadherin-PAR3-alpha-catenin complex regulates the Golgi localization and activity of cytosolic phospholipase A(2)alpha in endothelial cells.( PMID: 22398721
    30. genetic association studies in a Chinese Han population: Data suggest that 6 SNPs in PARD3 (rs2496720, rs2252655, rs3851068, rs118153230, rs10827337, rs12218196) are associated with neural tube defects/anencephaly in this population. PMID: 22447895
    31. The scaffolding adaptor GAB1 interacts with two polarity proteins, PAR1 and PAR3. PMID: 22883624
    32. Changes in cell polarity proteins Par-3 and PP-1 are associated with altered expression and assembly of tight junction proteins claudin-2, -3, -5 and -7 and ZO-1, causing paracellular leakage in active coeliac disease. PMID: 21865402
    33. there was no association of MAGI2 and PARD3 with IBD. PMID: 21515326
    34. Factors-derived from preeclapsia placentas not only interrupt junction protein VE-cadherin distribution, but also perturb polarity protein PARD-3 expression and distribution in vascular endothelium PMID: 20959641
    35. Data show that DDR1 coordinates the Par3/Par6 cell-polarity complex through its carboxy terminus, binding PDZ domains in Par3 and Par6. PMID: 21170030
    36. Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins. PMID: 20933426
    37. The results suggest that Par3 directly interacts with FAK and PI3-kinase, enhancing their activities for polarized cell migration. PMID: 20191563
    38. Data show that Aurora A interacts directly with the atypical protein kinase C binding domain of Par3 and phosphorylates it at serine 962. PMID: 19812038
    39. findings demonstrated that G-protein-activated phospholipase C-beta interacts with cell polarity proteins Par3 and Par6 to form protein complexes and to mediate downstream signal transduction PMID: 15782111
    40. fine-tuning mechanism of Par3 in epithelial tight junction assembly controlled by the EGF receptor-SFK signaling pathway. PMID: 17053785
    41. We provide evidence for the existence of a distinct PAR protein complex in endothelial cells. Both PAR-3 and PAR-6 associate directly with the adherens junction protein vascular endothelial cadherin (VE-cadherin). PMID: 17057644
    42. Par3 is a novel component of the DNA-PK complex that implicates an unexpected link of cell polarity to double-strand DNA break repair. PMID: 17287830
    43. Because Numb interacts with the aPKC binding partner PAR-3, we propose a model in which polarized Numb phosphorylation contributes to cell migration by directing integrin endocytosis to the leading edge PMID: 17609107
    44. Two polarity proteins, Par3 and Par6, are also required for EC lumen and tube formation, as they establish EC polarity through their association with Cdc42 and atypical PKC. PMID: 18319301
    45. identified 10 potential novel binding proteins of PDZ domains of Par3 in Jurkat cells PMID: 18685789
    46. Results suggest that deletion and reduced expression of PARD3 may be a novel mechanism that drives the progression of ESCC. PMID: 19503097
    47. Cell polarity factor Par3 binds SPTLC1 and modulates monocyte serine palmitoyltransferase activity and chemotaxis PMID: 19592499
    48. Pard-3 and AS3 genes are mutationally inactivated in prostate cancer cells. PMID: 19737411

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  • 相關(guān)疾病：
    Neural tube defects (NTD)
  • 亞細(xì)胞定位：
    Cytoplasm. Endomembrane system. Cell junction. Cell junction, tight junction. Cell junction, adherens junction. Cell membrane. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    PAR3 family
  • 組織特異性：
    Widely expressed.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 16051

    OMIM: 182940

    KEGG: hsa:56288

    STRING: 9606.ENSP00000363921

    UniGene: Hs.131489