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  4. Recombinant Human Probable alpha-ketoglutarate-dependent dioxygenase ABH5 (ALKBH5)

Recombinant Human Probable alpha-ketoglutarate-dependent dioxygenase ABH5 (ALKBH5)

In Stock
  • 中文名稱：
  • 貨號(hào)：
    CSB-EP744062HU
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    ALKBH5
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 來(lái)源：
    E.coli
  • 分子量：
    48.2 kDa
  • 表達(dá)區(qū)域：
    2-394aa
  • 氨基酸序列
    AAASGYTDLREKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVDRAPLRNKYFFGEGYTYGAQLQKRGPGQERLYPPGDVDEIPEWVHQLVIQKLVEHRVIPEGFVNSAVINDYQPGGCIVSHVDPIHIFERPIVSVSFFSDSALCFGCKFQFKPIRVSEPVLSLPVRRGSVTVLSGYAADEITHCIRPQDIKERRAVIILRKTRLDAPRLETKSLSSSVLPPSYASDRLSGNNRDPALKPKRSHRKADPDAAHRPRILEMDKEENRRSVLLPTHRRRGSFSSENYWRKSYESSEDCSEAAGSPARKVKMRRH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
  • 基因功能參考文獻(xiàn)：
    1. Overexpression of ALKBH5 in MIA-PaCa-2 and BxPC-3 cells, demonstrats that ALKBH5 could inhibit cell migration and invasion and ALKBH5 inhibits cell motility by demethylating and increasing expression of KCNK15- AS1. PMID: 30032148
    2. N(6)-Methyladenosine itself serves as a 'conformational marker', which induces different conformational outcomes in RNAs depending on sequence context. This critically impacts its interactions with several m6A-recognising proteins, including FTO and ALKBH5. PMID: 27156733
    3. ALKBH5 knockdown in breast cancer cells significantly decreased metastasis from breast to lungs in immunodeficient mice PMID: 27590511
    4. ALKBH5-FOXM1 pathway is critical for glioblastoma proliferation and tumorigenesis.ALKBH5 expression increases in glioblastoma stem-like cells and predicts poor survival in glioblastoma patients. PMID: 28344040
    5. HIF-dependent ALKBH5 expression mediates enrichment of BCSCs in the hypoxic tumor microenvironment PMID: 27001847
    6. The ALKBH5 gene may play a role in conferring risk of MDD in the Chinese population. PMID: 26047305
    7. Structures of human ALKBH5 demethylase reveal a unique binding mode for specific single-stranded N6-methyladenosine RNA demethylation. PMID: 24778178
    8. Modelling substrate into the active site of ALKBH5 reveals conserved residues important for recognition and demethylation mechanisms. PMID: 24489119
    9. findings provide a structural basis for understanding the substrate recognition specificity of Alkbh5 and offer a foundation for selective drug design against AlkB members PMID: 24616105
    10. ALKBH5 is a RNA demethylase and its action strongly suggests that the reversible methyladenosine modification has fundamental and broad functions in mammalian cells. PMID: 23177736
    11. ALKBH5 may have a role in the regulation of cellular responses to hypoxia as a class of HIF-transcriptional target gene PMID: 21264265

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  • 亞細(xì)胞定位：
    Nucleus speckle.
  • 蛋白家族：
    AlkB family
  • 組織特異性：
    Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 25996

    OMIM: 613303

    KEGG: hsa:54890

    STRING: 9606.ENSP00000382091

    UniGene: Hs.744130