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  4. Recombinant Human Rho guanine nucleotide exchange factor 7 (ARHGEF7), partial

Recombinant Human Rho guanine nucleotide exchange factor 7 (ARHGEF7), partial

  • 中文名稱：
    Recombinant Human Rho guanine nucleotide exchange factor 7(ARHGEF7) ,partial
  • 貨號(hào)：
    CSB-EP618876HU
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    ARHG7; ARHG7_HUMAN; ARHGEF 7; Arhgef7; Beta pix; Beta-Pix; betaPix; betaPix-b; betaPix-c; Cool; COOL-1; COOL1; DKFZp686C12170; DKFZp761K1021; KIAA0142; KIAA0412; mKIAA0142; Nbla10314; P50; P50BP; p85; P85COOL1; P85SPR; PAK interacting exchange factor beta; PAK-interacting exchange factor beta; PAK3; Pak3bp; PIX; PIXB; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor (GEF) 7b; Rho guanine nucleotide exchange factor (GEF7); Rho guanine nucleotide exchange factor 7; SH3 domain containing proline rich protein
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    54.8kDa
  • 表達(dá)區(qū)域：
    1-250aa
  • 氨基酸序列
    MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKVYPEPRSESECLSNIREFLRGCGASLRLELLFPPSQPPQHLVTTILLSASTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal GST-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
  • 基因功能參考文獻(xiàn)：
    1. Traction forces generated by beta-PIX knockdown cells are increased relative to those in control cells, a result consistent with an unexpected enhancement in the migration of single beta-PIX knockdown cells and monolayers of such cells. Authors propose that targeting beta-PIX might be a means of promoting epithelialization of wounds in vivo. PMID: 28596238
    2. S340E mutation enhances Nox1 activation (Kaito et al., 2014), the present study suggests that betaPix can also play an inhibitory role in O2(-) production, depending on the sites of phosphorylation. PMID: 29242061
    3. Data suggest that Scribble PDZ-domain-1 and PDZ-domain-3 are major domains that interact with beta-PIX and exhibit distinct binding hierarchy in interactions between individual Scribble PDZ domains and beta-PIX. (Scribble = scribbled planar cell polarity protein; beta-PIX = Rho guanine nucleotide exchange factor 7) PMID: 29061852
    4. each mutation in LRRK2 and ARHGEF7 demonstrated its unique functional property, which may contribute to the pathogenesis of Parkinson disease PMID: 27423549
    5. we discuss recent findings in key physiological systems that exemplify current understanding of the function of this important regulatory complex. Further, we draw attention to gaps in crucial information that remain to be filled to allow a better understanding of the many roles of the GIT-PIX complex in health and disease PMID: 27182061
    6. Data show association of G protein-coupled receptor kinase-interacting protein 1 (GIT1), p21-activated kinase interacting exchange factor (betaPIX), and p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1) with centrosomes. PMID: 27012601
    7. Data show the role of Rho guanine nucleotide exchange factor 7 (beta-PIX) to the regulation of high mobility of lung adenocarcinoma cell line H1299 via regulation of focal adhesions dynamics, changes in actin cytoskeleton organization and cell polarity. PMID: 25683605
    8. Phosphorylation of LRRK2 by casein kinase 1alpha regulates trans-Golgi clustering via differential interaction with ARHGEF7. PMID: 25500533
    9. GIT1/betaPIX/Rac1/PAK pathway plays a crucial role in regulating GABA(A)R synaptic stability and hence inhibitory synaptic transmission with important implications for inhibitory plasticity and information processing in the brain. PMID: 25284783
    10. Conversely, increased expression of betaPIX in breast cancer cell lines re-couples the Hippo kinase cassette to Yap/Taz, promoting localization of Yap/Taz to the cytoplasm and inhibiting cell migration and proliferation. PMID: 25425573
    11. Data indicate that suppression of c-Cbl protein by rho guanine nucleotide exchange factor 7 (Cool-1) may be critical for generation of at least a subset of glioblastoma (GBM). PMID: 24458840
    12. The interaction of betaPix with srGAP1 is critical for maintaining suppressive crosstalk between Cdc42 and RhoA during 3D collagen migration. PMID: 25150978
    13. BetaPix phosphorylation at Ser-340 upregulates Nox1 through Rac activation. PMID: 24792722
    14. It has identified and characterized a novel interaction between CaM and beta-p21-activated kinase interacting exchange factor (beta-PIX), a putative guanine exchange factor implicated in cell signaling. PMID: 22588125
    15. Inhibition of glycogen synthase kinase (GSK)-3beta increases the migration capacity of mesenchymal stromal cells during ex vivo expansion. PMID: 23288365
    16. beta1Pix functions as a transcriptional regulator of beta-catenin signaling through direct interaction with beta-catenin, an action that may be functionally relevant to colon cancer biology. PMID: 24129564
    17. BETA-PIX is a novel downstream signalling mediator during invadopodia formation. PMID: 23740575
    18. In vivo migratory capacity of mesenchymal stromal cells depends upon the expression level of beta-PIX. PMID: 22087847
    19. These defects could be rescued by depletion of ARHGEF7 and p21-activated kinase, Rac1-specific effector proteins required for cell adhesion. PMID: 22945935
    20. a model by which SNX27 regulates trafficking of beta-Pix to focal adhesions and thereby influences cell motility. PMID: 21926430
    21. Results describe the role of beta-Pix in the negative regulation of focal adhesion maturation and the promotion of lamellipodial protrusion and focal adhesion turnover to drive cell migration. PMID: 21423176
    22. Downstream effects of phosphorylation of ARHGEF7 through LRRK2 could be (i) a feedback control mechanism for LRRK2 activity as well as (ii) an impact of LRRK2 on actin cytoskeleton regulation PMID: 21048939
    23. betaPix up-regulates NHE3 membrane expression and activity by Shank2-mediated protein-protein interaction and by activating Rho GTPases in the apical regions of epithelial cells PMID: 20080968
    24. MYO18A is a novel binding partner of the PAK2/betaPIX/GIT1 complex and suggest that MYO18A may play an important role in regulating epithelial cell migration via affecting multiple cell machineries. PMID: 19923322
    25. bFGF- and NGF-induced phosphorylation of p85 betaPIX mediates Rac1 activation, which in turn regulates cytoskeletal reorganization at growth cones, but not translocation of the PIX complex. PMID: 14557270
    26. PIX protein is tightly associated with the GIT family ARF GTPase-activating proteins as a multimeric nexus capable of linking together important signaling molecules. PMID: 15212761
    27. PAK1 recruitment to the T cell-antigen-presenting cell interface required interaction with PIX. PMID: 15864311
    28. These results suggest that the formation of the complex consisting of Nox1, betaPix, and NoxO1 is likely to be a critical step in EGF-induced ROS generation. PMID: 16329988
    29. the SH3 domain of betaPix specifically interacts with a proline-arginine motif (PxxxPR) present within the ubiquitin ligase Cbl and Pak1 kinase. Cdc42/betaPix complex blocks Cbl's ability to downregulate EGFR. PMID: 16407834
    30. Rac1-beta-Pix interaction is required for Rac1 activation by beta-Pix as well as for Rac1-mediated spreading PMID: 16492808
    31. These data strongly suggest that, in addition to the known SAP-interacting kinase Fyn, PIX may be another key player in SAP-mediated T cell activation. PMID: 16983070
    32. Tiam1 and betaPIX mediate OxPAPC-induced Rac activation, cytoskeletal remodeling, and barrier protective response in pulmonary endothelium PMID: 17219408
    33. The association of PLCgamma1 with complexes containing GIT1 and beta-Pix is essential for its role in integrin-mediated cell spreading and motility. As a component of this complex, PLCgamma1 is also involved in the activation of Cdc42 and Rac1. PMID: 17562871
    34. PKA-dependent phosphorylation modulates PIXB activity through 14-3-3-beta binding. PMID: 18160719
    35. mutant betaPIX lacking guanine nucleotide exchange factor activity inhibited lamellipodium formation PMID: 18325335
    36. These results identify p66Shc and FOXO3a as novel partners of beta(1)Pix and represent the first direct evidence of beta(1)Pix in cell proliferation via Erk/p66Shc-dependent and Akt-independent mechanisms. PMID: 18385518
    37. Data show that the Rac1 guanine exchange factor- beta-Pix, localizes to focal contacts in human primary Schwannoma cells. PMID: 18445079
    38. Beta-PIX regulates nitric oxide synthase type 2 (NOD2) trafficking and NOD2-dependent signal transduction in primary human monocytes and cell line THP-1. PMID: 18684957
    39. betaPIX and GIT1 regulate the hepatocyte growth factor-induced and Rac1-dependent membrane transport of WAVE2 and consequently, lamellipodia formation. PMID: 19303398

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  • 亞細(xì)胞定位：
    Cell junction, focal adhesion. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection, lamellipodium. Note=Detected at cell adhesions. A small proportion is detected at focal adhesions.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 15607

    OMIM: 605477

    KEGG: hsa:8874

    STRING: 9606.ENSP00000364893

    UniGene: Hs.508738