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  4. Recombinant Human Ribonuclease T2 (RNASET2)

Recombinant Human Ribonuclease T2 (RNASET2)

  • 中文名稱：
    人RNASET2重組蛋白
  • 貨號：
    CSB-YP019810HU
  • 規(guī)格：
    ￥2208
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    RNASET2
  • Uniprot No.：
  • 別名：
    Ribonuclease 6
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Yeast
  • 分子量：
    30.9 kDa
  • 表達區(qū)域：
    25-256aa
  • 氨基酸序列
    DKRLRDNHEWKKLIMVQHWPETVCEKIQNDCRDPPDYWTIHGLWPDKSEGCNRSWPFNLEEIKDLLPEMRAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQVDALNSQKKYFGRSLELYRELDLNSVLLKLGIKPSINYYQVADFKDALARVYGVIPKIQCLPPSQDEEVQTIGQIELCLTKQDQQLQNCTEPGEQPSPKQEVWLANGAAESRGLRVCEDGPVFYPPPKKTKH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    C-terminal 6xHis-Myc-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    25-35 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Ribonuclease that plays an essential role in innate immune response by recognizing and degrading RNAs from microbial pathogens that are subsequently sensed by TLR8. Cleaves preferentially single-stranded RNA molecules between purine and uridine residues, which critically contributes to the supply of catabolic uridine and the generation of purine-2',3'-cyclophosphate-terminated oligoribonucleotides. In turn, RNase T2 degradation products promote the RNA-dependent activation of TLR8. Plays also a key role in degradation of mitochondrial RNA and processing of non-coding RNA imported from the cytosol into mitochondria. Participates as well in degradation of mitochondrion-associated cytosolic rRNAs.
  • 基因功能參考文獻：
    1. High RNASET2 expression is associated with reduced sperm motility. PMID: 29581387
    2. Studied association of and RNASET2, GPR174, and PTPN22 gene polymorphisms and liver damage(LD) due to Graves' disease (GD) hyperthyroidism. Found GPR174 rs3827440, PTPN22 rs3789604, and RNASET2 rs9355610 were significantly associated with altered GD-derived LD risk. PMID: 28568286
    3. RNASET2 is the enzyme that degrades the RNAs PMID: 28730546
    4. RNASET2, an IBD susceptibility gene, is a component of TL1A-mediated pathways regulating cytokine production. PMID: 28400196
    5. inhibits melanocyte outgrowth through interacting with shootin1; this effect may be associated with vitiligo pathogenesis PMID: 26293343
    6. Biological features allow to put forward the hypothesis that the RNASET2 protein can act as a molecular barrier for limiting the damages and tissue remodeling events occurring during the earlier step of cell transformation. PMID: 25797262
    7. RNASET2 may contribute to the development of vitiligo by inhibiting TNF Receptor-Associated Factor 2 expression and lead directly to apoptosis of melanocytes PMID: 26067323
    8. describe a multi-step strategy that allows production of highly pure, catalytically competent recombinant RNASET2 in both wild-type and mutant forms PMID: 25663099
    9. RNASET2 has antitumorigenic and antiangiogenic activities; a truncated version of human RNASET2, starting at E50 (trT2-50) and devoid of ribonuclease activity, has actin binding and anticancer-related biological activities PMID: 25426551
    10. RNASET2 tag SNP but not CCR6 polymorphisms is associated with autoimmune thyroid diseases in the Chinese Han population PMID: 25928629
    11. Results show that downregulation of RNASET2 and GGNBP2 in drug-resistant ovarian cancer tissues/cells contributes to the regulation of drug resistance in ovarian cancer. PMID: 24842157
    12. RNASET2 contributes to vitiligo pathogenesis by inhibiting TRAF2 expression. PMID: 24457966
    13. Genotypes of single nucleotide polymorphisms (SNPs) in RNASET2 gene were determined. PMID: 24327149
    14. Ribonuclease T2, an ancient and phylogenetically conserved RNase, has a role in development of ovarian neoplasms PMID: 23630276
    15. Higher expression of RNASET2 in the semen of asthenozoospermia individuals may contribute to sperm motility impairment. PMID: 23258633
    16. RNASET2--an autoantigen in anaplastic large cell lymphoma identified by protein array analysis. PMID: 22732457
    17. The catalytic features of RNase T2 in presence of bivalent cations were analyzed and the structural consequences of known clinical mutations were investigated. PMID: 22735700
    18. a possible involvement of RNASET2 in P-body formation in mammalian cells. PMID: 22188480
    19. Tax represses expression of RNase T2. PMID: 21994792
    20. Molecular signature induced by RNASET2, a tumor antagonizing gene, in ovarian cancer cells. PMID: 21646684
    21. The expression of the human tumor suppressor protein RNASET2 was studied in baculovirus-insect cell and Pichia pastoris heterologous systems. PMID: 21446958
    22. Familial cystic leukoencephalopathy arising in RNASET2-deficient humans is a manifestation of an lysosomal storage disorders in which rRNA is the best candidate for the noxious storage material. PMID: 21199949
    23. RNaseT2 is a cell growth regulator and it does not induce senescence in SV40 immortalized cell lines. PMID: 19382914
    24. RNASET2 found to significantly decrease the metastatic potential of ovarian cancer cell line in vivo; tumor suppression by RNASET2 is suggested to not be mediated by its ribonuclease activity PMID: 15809705
    25. The results presented herein represent a further advancement toward the molecular understanding of the tumour suppressive properties of the human RNASET2 protein. PMID: 16620762
    26. Loss of RNASET2 is associated with melanoma PMID: 18543608
    27. Study shows that loss-of-function mutations in the gene encoding the RNASET2 glycoprotein lead to cystic leukoencephalopathy, an autosomal recessive disorder with an indistinguishable clinical and neuroradiological phenotype. PMID: 19525954

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  • 相關疾?。?/div>
    Leukoencephalopathy, cystic, without megalencephaly (LCWM)
  • 亞細胞定位：
    Secreted. Lysosome lumen. Endoplasmic reticulum lumen. Mitochondrion intermembrane space.
  • 蛋白家族：
    RNase T2 family
  • 組織特異性：
    Ubiquitous. Higher expression levels observed in the temporal lobe and fetal brain.
  • 數據庫鏈接：

    HGNC: 21686

    OMIM: 612944

    KEGG: hsa:8635

    STRING: 9606.ENSP00000422846

    UniGene: Hs.529989