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  4. Recombinant Human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform (PPP2R2B)

Recombinant Human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform (PPP2R2B)

Promotion
  • 中文名稱：
    人PPP2R2B重組蛋白
  • 貨號：
    CSB-EP018565HU
  • 規(guī)格：
    ￥1836
  • 促銷：
    現(xiàn)貨重組蛋白特價促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    56.7 kDa
  • 表達區(qū)域：
    1-443aa
  • 氨基酸序列
    MEEDIDTRKINNSFLRDHSYATEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQDKVN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.
  • 基因功能參考文獻：
    1. Dephosphorylation and total levels of beta-catenin were dependent on PR55beta in an adenoid cystic carcinoma tumor cell line. PMID: 29275362
    2. New pathogenic threshold of PPP2R2B CAG-43 to be pathogenic for SCA12 diagnosis. PMID: 27864267
    3. CAG triplet repeat expansion has been found in untranslated region of SCA12 gene in Indian spinocerebellar ataxia 12 patients. PMID: 27350687
    4. Thus, Fcp1 coordinates Cdk1 and Gwl inactivation to derepress PP2A-B55, generating a dephosphorylation switch that drives mitosis progression. PMID: 26653855
    5. investigated CAG copies within PPP2R2B in 29 patients with spinocerebellar ataxia who are excluded from the most common subtypes; 3 patients carried 53, 46 and 54 CAG repeats respectively, while the other 26 cases harbored CAG repeats less than 30 PMID: 25634432
    6. IER5 functions as a positive feedback regulator of HSF1 and that this process involves PP2A/B55 and HSF1 dephosphorylation. PMID: 25816751
    7. Bcl-2 phosphorylation at Ser70 by selective tyrosine nitration of PP2A-B56delta stabilizes its antiapoptotic activity PMID: 25082878
    8. Inactivation of protein-phosphatase 2A causing hyperphosphorylation of paraprotein targets in monoclonal gammopathy of undetermined significance, multiple myeloma and Waldenstrom's macroglobulinemia is due to an exchange of its regulatory subunits PMID: 24676687
    9. We demonstrate that PP2A/B55 is required for Gwl dephosphorylation at the essential Cdk site Thr194. Ensa/ARPP19 dephosphorylation is mediated by the RNA PMID: 24391510
    10. PP2A-B55beta promotes cyclin E1 overexpression by antagonizing its degradation and its inhibition could represent a therapeutic mechanism for abrogating cyclin E1 function in cancers PMID: 24509904
    11. Bod1 regulates protein phosphatase 2A at mitotic kinetochores. PMID: 24157919
    12. Differential autophagic neuronal cell death under stress is determined by the cytoplasmic and mitochondrial-specific PPP2R2B isoforms. PMID: 23381641
    13. Single nucleotide polymorphisms in PPP2R2B is associated with drug response in breast cancer. PMID: 23034890
    14. Data indicate that PPP2R2B gene product PP2A/Bbeta2 activates the mitochondrial fission enzyme dynamin-related protein 1 (Drp1) by dephosphorylating Ser656. PMID: 22583914
    15. This study suggested that PPP2R2B CAG expansion mutation might lead increase induction of Spinocerebellar ataxia type 12. PMID: 21827912
    16. defective production of PP2A Bbeta upon IL-2 deprivation results in apoptosis resistance and longer survival of autoreactive T cells, in a subset of SLE patients PMID: 21746932
    17. oxidative stress induced by mitochondrial dysfunction causes elevated expression of ppp2r2b and plays a causal role in SCA12; and reduction of ROS is a potential therapeutic intervention for this neuropathy PMID: 21471219
    18. results indicate that the A variant of the rs319217 SNP of the PPP2R2B gene is a marker of better prognosis in breast cancer PMID: 20669227
    19. CAG repeat lengths in the PPP2R2B gene may be potential genetic markers for Alzheimer disease susceptibility in the Japanese population. PMID: 21029765
    20. PPP2R2B, encoding the B55beta regulatory subunit of the PP2A complex, is epigenetically inactivated by DNA hypermethylation in colorectal cancer. PMID: 21075311
    21. Autosomal dominant cerebellar ataxia mapping to 5q31-q33.1 has no CAG repeat expansion or other mutations of the PPP2R2B gene. PMID: 20937954
    22. Quantitative methylation analysis identified ABCB1, FOXC1, PPP2R2B and PTEN as novel genes to be methylated in ductal carcinoma in situ. PMID: 20056007
    23. The CAG repeat in SCA12 functions as a cis element to up-regulate PPP2R2B expression. PMID: 20533062
    24. We investigated methylation patterns in the promoter regions of ABCB1, ATM, BRCA1, CDH3, CDKN2A, CXCR4, ESR1, FBXW7, FOXC1, GSTP1, IGF2, HMLH1, PPP2R2B, and PTEN75 in well-described pre-treatment samples from locally advanced breast cancer PMID: 20338046
    25. Cells with ectopically expressed PPP2R2B were shown predisposed to autophagy and oxidative stress induced cell death. PMID: 20017961
    26. a novel ATM-dependent mechanism is regulating association of B55 subunits with nuclear PP2A in response to IR PMID: 11723136
    27. Cyclin G recruits PP2A in order to modulate the phosphorylation of Mdm2 and thereby to regulate both Mdm2 and p53. PMID: 11983168
    28. Phosphorylation of bestrophin is regulated by PP2A. PMID: 12058047
    29. SCA12 triplet expansion rate in Italian ataxia patients PMID: 12140678
    30. Analysis of CTA/CTG and CAG polymorphism in a Polish control group. PMID: 14960773
    31. phospholipase D suppresses protein phosphatase 2A and is involved in the mTOR survival pathway in the transformation of human cells PMID: 16109716
    32. PP2A plays a positive rather than a negative role in the regulation of IKKbeta PMID: 16126728
    33. analysis of a founder for SCA12 in the Indian population PMID: 16138911
    34. We therefore propose that PP2A plays a crucial role in the maintenance of cell-cell adhesion through recruitment of IQGAP1 to the Rac-bound E-cadherin-catenins complex. PMID: 16245300
    35. The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein. PMID: 16286244
    36. PP2A-mediated dephosphorylation of HSP27 and tau correlated with PP2A-induced preservation of endothelial cell cytoskeleton PMID: 16475161
    37. Cyclin G2 may modulate the cell cycle and cellular division processes through modulation of PP2A and centrosomal associated activities. PMID: 17123511
    38. The small t antigen (ST) of DNA tumor virus SV40 inhibits PP2A phosphatase activity through its N-terminal J domain. PMID: 17529992
    39. results suggest that the PPP2R2B gene CAG repeat polymorphism may be functional and may, in part, play a role in conferring susceptibility to Alzheimer disease and essential tremor in Taiwan PMID: 18484086
    40. Adenovirus E4orf4 protein downregulates MYC expression through interaction with the PP2A-B55 subunit PMID: 18653458
    41. Results suggest that Parkinsonism (PD) and multiple system atrophy (MSA) are not associated with spinocerebellar ataxia 12 (SCA12) and it is not necessary to screen SCA12 in PD and MSA patients. PMID: 18973067
    42. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826

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  • 相關疾?。?/div>
    Spinocerebellar ataxia 12 (SCA12)
  • 亞細胞定位：
    [Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Membrane.; [Isoform 2]: Cytoplasm. Mitochondrion. Mitochondrion outer membrane.
  • 蛋白家族：
    Phosphatase 2A regulatory subunit B family
  • 組織特異性：
    Brain.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9305

    OMIM: 604325

    KEGG: hsa:5521

    STRING: 9606.ENSP00000336591

    UniGene: Hs.627618