Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Human Short transient receptor potential channel 6 (TRPC6), partial

Recombinant Human Short transient receptor potential channel 6 (TRPC6), partial

Promotion
  • 中文名稱：
    人TRPC6重組蛋白
  • 貨號：
    CSB-MP1339HU
  • 規(guī)格：
    ￥3960
  • 促銷：
    現(xiàn)貨重組蛋白特價促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 來源：
    Mammalian cell
  • 分子量：
    34.7 kDa
  • 表達區(qū)域：
    543-592aa
  • 氨基酸序列
    ARFMAFWHASKAQSIIDANDTLKDLTKVTLGDNVKYYNLARIKWDPSDPQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    C-terminal hFc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • 基因功能參考文獻：
    1. TRPC6 was upregulated in Diabetic nephropathy and could promote cell proliferation and inflammation by inhibiting the NFAT signaling pathway in tubular epithelial cells. PMID: 29288897
    2. Changes in podocyte TRPC channels evoked by plasma and sera from patients with recurrent FSGS and by putative glomerular permeability factors. PMID: 28629718
    3. Confirmed serine 14 as a target of MAPKs and proline-directed kinases like cyclin-dependent kinase 5 (Cdk5) in cell-based as well as in vitro kinase assays and quantitative phosphoproteomic analysis of TRPC6. Phosphorylation of TRPC6 at serine 14 enhances channel conductance by boosting membrane expression of TRPC6, whereas protein stability and multimerization of TRPC6 are not altered. PMID: 28877958
    4. Reduction of TRPC6 activity, using either TRPC6 siRNA or a TRPC6 blocker, led to inhibition of hypoxia-induced autophagy, while enhancement of TRPC6 activity with a TRPC6 activator resulted in increased hypoxia-induced autophagy. PMID: 30078002
    5. axonal colocalization of TRPV4 and TRPC6 was found in the digital Meissner corpuscles PMID: 27874267
    6. Data suggest that TRPC6-mediated elevation of intracellular Ca2+ stimulates non-small cell lung cancer proliferation by promoting cell cycle progression. PMID: 28030826
    7. potential implications of transient receptor potential (TRP) channels in the pathogenesis of intestinal fibrosis, since they are known to act as cellular stress sensors/transducers affecting intracellular Ca(2+) homeostasis/dynamics, and are involved in a broad spectrum of cell pathophysiology including inflammation and tissue remodeling. PMID: 27818466
    8. Studies provide evidence that the TRPC6-mediated signaling pathway in kidney cells is under control of reactive oxygen species under both physiological and pathological conditions. [review] PMID: 26937558
    9. Functional interaction of upregulated CaSR and upregulated TRPC6 in pulmonary artery smooth muscle cells from idiopathic pulmonary arterial hypertension patients may play an important role in the development and progression of sustained pulmonary vasoconstriction and pulmonary vascular remodeling. PMID: 26968768
    10. Our comprehensive analysis of human disease-causing TRPC6 mutations reveals loss of TRPC6 function as an additional concept of hereditary focal segmental glomerulosclerosis and provides molecular insights into the mechanism responsible for the loss-of-function phenotype of TRPC6 G757D in humans PMID: 26892346
    11. study demonstrated that the various mechanisms regulating MDR in HCC cells are calcium dependent through the TRPC6/calcium/STAT3 pathway. We propose that targeting TRPC6 in HCC may be a novel antineoplastic strategy, especially combined with chemotherapy PMID: 27011063
    12. n response to stretching (20%), ATP was released only from the foremost cells at the wound edge; it then diffused to the cells behind the wound edge and activated the P2Y receptors, which caused propagating Ca(2+) waves via TRPC6 PMID: 28210627
    13. Data suggest that targeted manipulation of protein kinase C isoforms PKCalpha, PKCbeta, and PKCeta might be beneficial in certain proteinuric kidney diseases with altered transient receptor potential cation channel subfamily C member 6 protein (TRPC6) functions. PMID: 26404773
    14. Insulin increases the expression of TRPC6 channels in podocytes by activation of the calcineurin-dependent pathway. PMID: 26849622
    15. This study described the expression and functional relevance of TRPC6 in the pathophysiology of HK-2 cell following ischemia reperfusion. PMID: 26913924
    16. Genetic Interactions Between TRPC6 and NPHS1 Variants Affect Posttransplant Risk of Recurrent Focal Segmental Glomerulosclerosis. PMID: 26147534
    17. These findings suggest that lysoPC induces CaM phosphorylation at Tyr(99) by a Src family kinase and that phosphorylated CaM activates PI3K to produce PIP3, which promotes TRPC6 translocation to the cell membrane. PMID: 26858457
    18. This study demonstrated that TRPC6 reduction or haploinsufficiency leads to altered neuronal development, morphology and function. PMID: 25385366
    19. TRPC6 specifically interacts with APP leading to inhibition of its cleavage by gamma-secretase and reduction in Abeta production. PMID: 26581893
    20. results suggest that TRPC6 regulates metabolism to affect HIF-1alpha stability and consequent glucose metabolism in human glioma cells under hypoxia PMID: 26187851
    21. the transient receptor potential canonical-6 (TRPC6) calcium-permeable channel in the alveolar macrophages also functions to shunt the transmembrane potential generated by proton pumping. PMID: 26604306
    22. TRPC6 genetic variants are promising candidate predictors of nervous system involvement in systemic lupus erythematosus PMID: 26531690
    23. Selectively activating endothelial TRPC6 rescues transendothelial migration PMID: 26392222
    24. TRPC6 plays a prominent role in thrombin-evoked delta-granule platelet exocytosis and calcium mobilization. PMID: 26386308
    25. This study seems to suggest that c.1-361A > T, c.1-254C > G and c.1-218C > T polymorphisms in TRPC6 gene and c.1166A > C polymorphism in AGTR1 could have a role in the development of this disease. PMID: 25603901
    26. distribution and activity of TRPC6 can be regulated by cardiotonic steroids like ouabain and the naturally occurring peptide Abeta(1-40) which underlines the pathophysiological significance of these processes. PMID: 26348127
    27. mutation at N157T can lead to alteration in glycation whereas mutation at A404V was present at a ligand binding site PMID: 26127002
    28. these findings provide strong evidence for a role of immunophilins, including FKBP25 and FKBP38, in NCCE mediated by TRPC6. PMID: 26239116
    29. TRPC6 polymorphisms do not affect susceptibility to, or clinical outcomes of idiopathic membranous nephropathy. PMID: 25019165
    30. Exogenous H2O2 does not induce oxidative stress due to rapid degradation to produce O2 in the podocytes, but the oxygenated podocytes become sensitive to acute ethanol challenge and undergo apoptosis via a TRPC6-dependent elevation of intracellular Ca2+. PMID: 25601712
    31. Renal ischemia-reperfusion injury induced podocyte effacement and the upregulation of TRPC6 mRNA and protein expression. In in vitro experiments, oxygen glucose deprivation (OGD) treatment enhanced the expression of TRPC6 and TRPC6-dependent Ca2+ influx. PMID: 25896763
    32. a new pathway for TRPC6 activation by Phospholipase C epsilon PMID: 25521631
    33. TRPC6 activation and inactivation are regulated by PI(4,5)P2 hydrolysis. PMID: 24470487
    34. Studied the expression of TRPC6 among prostate cancer cells; experimental results showed that the overexpression of TRPC6 could promote the invasion ability of PC3 prostate cancer cells. PMID: 24418082
    35. High glucose modifies TRPC6 channels and ROS production via SDC-4 in human podocytes. PMID: 24942878
    36. the TRPC6 activation mainly occurs at lipid rafts, which is regulated by the mechanical cues of surrounding materials. PMID: 24397990
    37. -254C>G, a SNP underlying enhanced TRPC6 transcription and expression, may be correlated with the development of steroid resistance in Chinese children with idiopathic nephrotic syndrome. PMID: 23999069
    38. TRPC6 mechanical activation and activation evoked by DAG/ATP occur through distinct biophysical mechanisms, and provide support for the hypothesis that protein complexes containing wild-type TRPC6 subunits can be intrinsically mechanosensitive. PMID: 24598806
    39. High expression of TRPC6 mRNA was associated with the higher pT status. PMID: 23686700
    40. Expression of TRPC6 is markedly increased in renal cell carcinoma specimens and plays an important role in tumor cell proliferation. PMID: 23700295
    41. The triple mutation Arg852/Lys859/Arg860 exhibited significant disruption of the binding of S100A1 to TRPC6 implicating their involvement in the binding site. PMID: 23671622
    42. TRPC6 gain-of-function mutation is associated with late-onset focal segmental glomerulosclerosis. PMID: 23291369
    43. Increasing expression levels of the transient receptor potential cation channel 6 gene in the blood accompanies chronic elevation of intraocular pressure in primary open-angle glaucoma and may serve as a genetic biomarker PMID: 23566105
    44. Mutations of TRPC6 and ACTN4 occur in only a minor portion of Chinese familial familial focal segmental glomerulosclerosis patients. PMID: 23689571
    45. A novel frame shift mutation in TRPC6, D873fsX878 was found in a family with podocytopathy. PMID: 23663351
    46. ANP attenuates the inflammatory actions of histamine via endothelial GC-A/cGMP/cGKI signaling and inhibitory phosphorylation of TRPC6 channels. PMID: 23814119
    47. Data indicate that expression of mutant TRPC6 induces ERK1/2 activation via both cell-autonomous and non-cell-autonomous mechanisms. PMID: 23645677
    48. data show that TRPC6 is likely to be a target for 11q21-22.2 amplification that confers enhanced invasive behavior to head and neck squamous cell carcinomas cells. PMID: 23497198
    49. this paper defines a specific role of TRPC6 channels in CXCR2-induced intermediary chemotaxis. In particular, TRPC6-mediated supply of calcium appears to be critical for activation of downstream signaling components. PMID: 23636057
    50. an important role of NF-kappaB in a negative regulation of TRPC6 expression at the gene transcription level in kidney cells. PMID: 23525112

    顯示更多

    收起更多

  • 相關疾?。?/div>
    Focal segmental glomerulosclerosis 2 (FSGS2)
  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Transient receptor (TC 1.A.4) family, STrpC subfamily, TRPC6 sub-subfamily
  • 組織特異性：
    Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12338

    OMIM: 603652

    KEGG: hsa:7225

    STRING: 9606.ENSP00000340913

    UniGene: Hs.159003