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Recombinant Human Single-stranded DNA cytosine deaminase (AICDA)

  • 中文名稱：
    Recombinant Human Single-stranded DNA cytosine deaminase(AICDA),Yeast
  • 貨號：
    CSB-YP001487HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human Single-stranded DNA cytosine deaminase(AICDA),Yeast
  • 貨號：
    CSB-EP001487HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Human Single-stranded DNA cytosine deaminase(AICDA),Yeast
  • 貨號：
    CSB-EP001487HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Human Single-stranded DNA cytosine deaminase(AICDA),Yeast
  • 貨號：
    CSB-MP001487HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Activation induced cytidine deaminase; Activation induced deaminase; Activation-induced cytidine deaminase; AICDA; AICDA_HUMAN; AID; ARP 2; ARP2; CDA 2; CDA2; Cytidine aminohydrolase; HIGM2; Integrated into Burkitt's lymphoma cell line Ramos
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-198
  • 氨基酸序列
    MDSLLMNRRK FLYQFKNVRW AKGRRETYLC YVVKRRDSAT SFSLDFGYLR NKNGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT FVENHERTFK AWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

Can you please provide the following information regarding your product AICDA recombinant protein (CSB-MP001487HU):
What mammalian cell line is used for mammalian expression system?
Please let me know if you have any additional questions.

A:
Very nice to receive your inquiry.
Recombinant Human Single-stranded DNA cytosine deaminase(AICDA)
CSB-MP001487HU >> Mammalian cell
Expression Region: 1-198aa; Full length.
Tag information: Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL


Generally, we will use HEK 293 for the mammalian cell expression system. The cell has high growth rate, it's easy to be transfected, and has high expression of foreign protein.

靶點詳情

  • 功能：
    Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation (SHM), gene conversion, and class-switch recombination (CSR) in B-lymphocytes by deaminating C to U during transcription of Ig-variable (V) and Ig-switch (S) region DNA. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.
  • 基因功能參考文獻：
    1. this study reports the clinical and AID genetic features of six Iranian hyper IgM syndrome patients PMID: 30081731
    2. induction of AID expression would result in chromosomal translocations in the process of differentiation from B cell derived induced pluripotent stem cells. PMID: 28490810
    3. The distribution of AID and A3s in the epithelial cells as well as germinal centres. PMID: 29343743
    4. patients with AS expressed significantly higher levels of sv2 than HC. TNFi treatment restored the gene expression of the AID variants (FL, sv1, and sv2) in patients with AS. Therefore pre-existing TNFalpha-induced AID expression in B cells may play a role in the pathogenesis of AS. PMID: 28959900
    5. we reported high AID, low miR-181b and high miR-155 expression in de novo adult B-ALL patients. Univariate high AID or low miR-181b expression was an unfavorable prognostic factor. High AID with low miR-181b or with low miR-155 expression is better in predicting unfavorable OS than univariate factor. High AID with low miR-181b and low miR-155 expression confers worst prognosis. PMID: 28140712
    6. AID expression was increased in chronic lymphocytic leukemia patients with del17p or del11q who have poor prognosis. PMID: 28388279
    7. Results indicate activation-induced cytidine deaminase (AICDA) as a driver of epigenetic heterogeneity in B-cell lymphomas with potential significance for other tumors with aberrant expression of cytidine deaminases. PMID: 29335468
    8. The binding and catalytic behavior of purified AID was tested on DNA/RNA hybrid substrates bearing either random sequences or GC-rich sequences simulating Ig S regions. AID exhibited a higher affinity for binding DNA/RNA hybrid substrates made of S region sequences, than any other DNA substrates. In the absence of any other cellular processes or factors, AID itself favors binding and mutating S-region DNA/RNA hybrids. PMID: 29161581
    9. Expression of human AID or increased oxidative stress induces DNA breaks within human chromosomal translocation fragile zones. PMID: 29220655
    10. Data implicate intrinsic preference of AID for structured substrates and uncover the importance of G4 recognition and oligomerization of AID in class switch recombination. PMID: 28757211
    11. the His130Pro mutation allows the enzyme to retain its mutagenic activity and would prevent the interaction of AID with specific cofactors required for class switch recombination but not for somatic hypermutation. Thus, there would be no contradiction between maintaining the catalytic and mutagenic activity of AID and the presence of function defects at the genomic level. PMID: 27716525
    12. The authors found that the viral epsilon RNA and C-terminus of AID are required for AID-mediated hepatitis B virus RNA reduction. PMID: 28779685
    13. silencing of AID in human bone marrow cells skews differentiation toward myelomonocytic lineage. However, in contrast to Tet2 loss, Aid loss does not contribute to enhanced HSC self-renewal or cooperate with Flt3-ITD to induce myeloid transformation. Genome-wide transcription and differential methylation analysis uncover the critical role of Aid as a key epigenetic regulator PMID: 28077417
    14. AID protein is expressed in a large proportion of Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia cases at levels detectable by immunohistochemistry. PMID: 26980048
    15. These data, showing the direct targeting and induction of functional AID by EBNA3C, suggest a novel role for EBV in the etiology of B cell cancers, including endemic Burkitt lymphoma PMID: 27217538
    16. These findings indicated that TNF-alpha-induced AID expression is involved with class switch recombination in cancer. PMID: 25849121
    17. Finnish founder allele causing HIGM2 identified. PMID: 27142677
    18. Structural analysis of AID protein required in immunoglobulin diversification has been reported. PMID: 27258794
    19. this study reports a case of growth hormone deficiency with an autosomal recessive Hyper-immunoglobulin M syndrome by phenotype and genotype, with a novel mutation in AICDA that has not been reported formerly PMID: 27789066
    20. We also report that AID activity results in epigenetic, genetic and genomic damage in fallopian tube epithelial cells PMID: 26936395
    21. AICDA/APOBEC family of cytidine deaminases is significant in innate immunity, as it restricts numerous viruses, including HBV, through hypermutationdependent and independent mechanisms. (Review) PMID: 26398702
    22. DNA methylation dynamics of germinal center B cells are mediated by AID. PMID: 26365193
    23. Mutations in activation-induced cytidine deaminase is associated with indolent chronic lymphocytic leukaemia. PMID: 26638776
    24. Data suggest novel mechanism in innate immunity allows cytokine TGF-beta to restrict viral circular DNA in hepatocyte nuclei via innate immunity; AID deaminates circular DNA of hepatitis B virus leading to DNA degradation; mechanism depends on UNG. PMID: 26867650
    25. The high levels of memory and activated B cells and follicular helper T cells were positively associated with the progression of immunoglobulin A nephropathy. This may be mediated by the overexpression of AID, which is potentially regulated by IL21. PMID: 26166388
    26. identify AID derivatives that accelerate somatic hypermutation with minimal impact on viability, which will be useful tools for engineering genes and proteins by iterative mutagenesis and selection PMID: 26355458
    27. High expression of activation-induced cytidine deaminase is associated with diffuse large B cell lymphoma. PMID: 26077666
    28. AID mutations leading to AID deficiency are the most frequent underlying molecular basis of hyper IgM syndrome in consanguineous Tunisian patients. PMID: 26545377
    29. Studies indicate that gene conversion mediated by activation-induced cytidine deaminase (AID) has been found to contribute to generation of the primary antibody repertoire. PMID: 26537386
    30. aberrant expression of AID may reflect continuous B cell activation and sustained survival signals in HCV-related CV patients. PMID: 26219420
    31. Surges in ERK activity induced by extracellular cues enhance Arp2/3-mediated actin polymerization to generate protrusion power phases sufficient to promote sustained cell motility. PMID: 25990957
    32. Data show that microRNAs miR-155 and miR-16 downregulate activation-induced cytidine deaminase (AID) and transcription factor E47 in B Cells through binding of the 3'-untranslated regions. PMID: 26223652
    33. Data indicate that both the amount and intensity of the activation-induced cytidine deaminase (AID) protein expression increased with the progression from precancerous to cancerous lesions in pancreatic ductal adenocarcinoma (PDAC) tissues. PMID: 26113087
    34. B cells hijack to supply ssDNA substrates for AID to mutate at immunoglobulin V regions during somatic hypermutation. PMID: 24923561
    35. HSP90 inhibitors indirectly target AID in vivo PMID: 25912253
    36. findings suggest that AID has a role in the development of oral epithelial dysplasia and promotes progression of oral cancer PMID: 24351917
    37. hnRNP K and hnRNP L may serve as A1CF-like cofactors in AID-mediated class switch recombination and somatic hypermutation PMID: 25902538
    38. Activation-induced cytidine deaminase (AID)-induced mutagenic U:G mismatches in DNA may be a fundamental and common cause of mutations in B-cell malignancies. PMID: 25486549
    39. In yeast, AID and the catalytic domain of APOBEC3G preferentially mutate transcriptionally active genes within narrow regions, 110 base pairs in width, fixed at RNA polymerase initiation sites. PMID: 25237741
    40. There was no significant association between the previously reported genetic variant of AICDA gene and the development of Common variable immunodeficiency or selective IgA deficiency PMID: 23731676
    41. demonstrates that intensity of malaria transmission correlates with AID expression levels in the presence of Epstein-Barr virus PMID: 25099163
    42. Study reports that the majority of detectable AID off-target activity in a variety of mouse and human lymphoid or nonlymphoid cell types occurs within focal regions of overlapping sense/antisense transcription within intragenic super enhancers. PMID: 25483776
    43. AID activity and its relationship to chromosome folding and the B cell regulome was studied; find that AID-mediated lesions occur predominantly within B cell super-enhancers and regulatory clusters; further show that the structural and transcriptional features of these domains help explain AID tumorigenic activity in the B cell compartment of mice and humans. PMID: 25483777
    44. data demonstrate that AID is active in CLL in vivo and thus, AID likely contributes to clonal evolution of CLL. PMID: 25179679
    45. analysis of a possible association between AID expression and BRAF mutation in melanoma PMID: 24684646
    46. Results show that both AID mRNA levels and DNA-cytosine deamination activities follow the same general pattern of increase and decline after stimulation in the two genetic backgrounds. PMID: 25154417
    47. DNA repair pathways which are in vitro activated by AID-induced lesions are reminiscent of those found during AID-induced in vivo immunoglobulin diversification. PMID: 24349193
    48. Results show that activation induced cytidine deaminase (AID) expression may be associated with deletions in patients with chronic lymphocytic leukemia (CLL). PMID: 23662991
    49. AID lacking the C terminus is impaired in its ability to recruit nonhomologous end joining repair factors, resulting in accumulation of double-strand breaks that undergo aberrant resection. PMID: 24973444
    50. findings reveal additional functions for AID in innate immune defense against KSHV with implications for a broader involvement in innate immunity to other pathogens. PMID: 24244169

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  • 相關(guān)疾?。?/div>
    Immunodeficiency with hyper-IgM 2 (HIGM2)
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Cytidine and deoxycytidylate deaminase family
  • 組織特異性：
    Strongly expressed in lymph nodes and tonsils.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 13203

    OMIM: 605257

    KEGG: hsa:57379

    STRING: 9606.ENSP00000229335

    UniGene: Hs.149342