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  4. Recombinant Human Speckle targeted PIP5K1A-regulated poly (A) polymerase (TUT1), partial

Recombinant Human Speckle targeted PIP5K1A-regulated poly (A) polymerase (TUT1), partial

  • 中文名稱：
    人TUT1重組蛋白
  • 貨號：
    CSB-YP884477HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人TUT1重組蛋白
  • 貨號：
    CSB-EP884477HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人TUT1重組蛋白
  • 貨號：
    CSB-EP884477HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人TUT1重組蛋白
  • 貨號：
    CSB-BP884477HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人TUT1重組蛋白
  • 貨號：
    CSB-MP884477HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TUT1
  • Uniprot No.：
  • 別名：
    alpha regulated poly(A) polymerase ; nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I; nuclear speckle targeted PIPK1A regulated poly(A) polymerase ; PAP associated domain containing 2 ; PAPD2; poly(A) polymerase associated domain containing 2 ; RBM21; RNA binding motif protein 21 ; RNA-binding motif protein 21; RNA-binding protein 21; Speckle targeted PIP5K1A-regulated poly(A) polymerase; Star-PAP; STARPAP; STPAP_HUMAN; terminal uridylyl transferase 1; U6 snRNA specific ; TUT1; TUTase; U6 snRNA specific terminal uridylyltransferase 1; U6 snRNA-specific terminal uridylyltransferase 1; U6 TUTase; U6-TUTase; URLC6
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
  • 基因功能參考文獻：
    1. Crystallographic and biochemical studies of TUT1 revealed the molecular mechanism underlying the specific oligo-uridylylation of the 3'-end of U6 snRNA by TUT1. PMID: 28589955
    2. Star-PAP possesses tumor-suppressing activity. PMID: 28151486
    3. Star-PAP-specific polyadenylation sites usage regulates the expression of the eukaryotic translation initiation factor EIF4A1, the tumor suppressor gene PTEN and the long non-coding RNA NEAT1. PMID: 28911096
    4. Star-PAP recognises a unique nucleotide motif on its target mRNA.CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding poly A polymerase. PMID: 26496945
    5. Nucleotidyl transferase TUT1 inhibits lipogenesis in osteosarcoma cells through regulation of microRNA-24 and microRNA-29a. PMID: 25142229
    6. Star-PAP controls E6 mRNA polyadenylation and expression and modulates wild-type p53 levels. PMID: 23416977
    7. The human TUT1 nucleotidyl transferase is a global regulator of microRNA abundance. PMID: 23874977
    8. Star-PAP and its regulatory molecules form a signaling nexus at the 3'-end of target mRNAs to control the expression of select group of genes including the ones involved in stress responses. (Review) PMID: 23306079
    9. PIPKIalpha, PI4,5P(2), and PKCdelta regulate Star-PAP control of BIK expression and induction of apoptosis. PMID: 22244330
    10. The data support a model where Star-PAP binds to the pre-mRNA, recruits the CPSF complex to the 3'-end of pre-mRNA and then defines cleavage by CPSF 73 and subsequent polyadenylation of its target mRNAs. PMID: 21102410
    11. PIPKIalpha co-localizes at nuclear speckles and interacts with a newly identified non-canonical poly(A) polymerase, Star-PAP; the activity of Star-PAP can be specifically regulated by PtdIns4,5P2 PMID: 18288197
    12. These data indicate that CKIalpha, PIPKIalpha, and Star-PAP function together to modulate the production of specific Star-PAP messages. PMID: 18305108

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  • 亞細胞定位：
    Nucleus, nucleolus. Nucleus speckle.
  • 蛋白家族：
    DNA polymerase type-B-like family
  • 組織特異性：
    Widely expressed.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 26184

    OMIM: 610641

    KEGG: hsa:64852

    STRING: 9606.ENSP00000308000

    UniGene: Hs.728983