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  4. Recombinant Human Taste receptor type 1 member 3 (TAS1R3), partial

Recombinant Human Taste receptor type 1 member 3 (TAS1R3), partial

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  • 中文名稱：
    人TAS1R3重組蛋白
  • 貨號(hào)：
    CSB-EP752534HU
  • 規(guī)格：
    ￥2328
  • 促銷：
    現(xiàn)貨重組蛋白特價(jià)促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    68.4 kDa
  • 表達(dá)區(qū)域：
    21-570aa
  • 氨基酸序列
    APLCLSQQLRMKGDYVLGGLFPLGEAEEAGLRSRTRPSSPVCTRFSSNGLLWALAMKMAVEEINNKSDLLPGLRLGYDLFDTCSEPVVAMKPSLMFLAKAGSRDIAAYCNYTQYQPRVLAVIGPHSSELAMVTGKFFSFFLMPQVSYGASMELLSARETFPSFFRTVPSDRVQLTAAAELLQEFGWNWVAALGSDDEYGRQGLSIFSALAAARGICIAHEGLVPLPRADDSRLGKVQDVLHQVNQSSVQVVLLFASVHAAHALFNYSISSRLSPKVWVASEAWLTSDLVMGLPGMAQMGTVLGFLQRGAQLHEFPQYVKTHLALATDPAFCSALGEREQGLEEDVVGQRCPQCDCITLQNVSAGLNHHQTFSVYAAVYSVAQALHNTLQCNASGCPAQDPVKPWQLLENMYNLTFHVGGLPLRFDSSGNVDMEYDLKLWVWQGSVPRLHDVGRFNGSLRTERLKIRWHTSDNQKPVSRCSRQCQEGQVRRVKGFHSCCYDCVDCEAGSYRQNPDDIACTFCGQDEWSPERSTRCFRRRSRFLAWGEPAVL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). TAS1R2/TAS1R3 recognizes diverse natural and synthetic sweeteners. TAS1R3 is essential for the recognition and response to the disaccharide trehalose. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
  • 基因功能參考文獻(xiàn)：
    1. Regarding "consumption of carbohydrates (% energy) and higher amount of sweet foods, respectively...no associations were found for the TAS1R3 alleles." PMID: 29110749
    2. TAS1R3 gene rs307355 polymorphism found independent risk factor for dental caries experience by logistic regression & increased risk of caries. Moderate caries (4-7 caries) found to be associated w/TAS1R3 rs307355 heterozygous genotype PMID: 25924601
    3. T1R3 gene expression in the tongue is suppressed by chemotherapy. PMID: 26422579
    4. The transcripts of TAS1R3 and UCN2 in peripheral blood cells may be considered potential biomarkers of consumption of sugary and fatty food, respectively, to complement data of food-intake questionnaires. PMID: 26168276
    5. Five amino acid residues in cysteine-rich domain of human T1R3 were involved in the response for sweet-tasting protein, thaumatin PMID: 23370115
    6. T1R3 is a receptor responsible for the detection of calcium by taste. PMID: 22773945
    7. Overexpressed the human N-terminal domain of T1R3 in E. coli and found that the refolded protein behaves as a dimer; showed that hT1R3-NTD is functional and capable of binding sucralose with an affinity in the millimolar range. PMID: 22450161
    8. amino acid substitutions (F749S and R757C), located in the transmembrane domain of T1R3, severely impair receptor functions in vitro PMID: 21422378
    9. The sweet taste receptors (alpha-gustducin and T1R3) are involved in glucose-stimulated secretion of GLP-1 and PYY. PMID: 21324568
    10. Allelic polymorphism within the TAS1R3 promoter is associated with human taste sensitivity to sucrose. PMID: 19559618
    11. The mechanism of interaction of the sweet protein monellin with T1R2-T1R3 receptor. PMID: 12706725
    12. cysteine-rich region of T1R3 has a role in determining responses to intensely sweet proteins PMID: 15299024
    13. An immunohistochemistry study to determine whether and where T1R3 may be localized in the liver and pancreas. PMID: 15585941
    14. transmembrane domains of human T1R3 bind with lactisole to inhibit sweet taste PMID: 15668251
    15. Although T1R2-T1R3 is known to have multiple potential ligand-binding sites to receive a wide variety of sweeteners, the present study is apparently the first to identify the ATD of hT1R3 as a new sweetener-binding region. PMID: 17499612
    16. These results show that T1R2 and T1R3 receptors, in addition to their role in taste perception, may also have a role in intrahepatic cholangiocytes. PMID: 17928076
    17. Expressed in the sweet/umami gustatory pathways in taste receptor cells and trigeminal neural pathways in transgenic mice. PMID: 18539481
    18. The heteromeric G-protein-coupled receptor T1R2:T1R3 responds to a vast array of chemically diverse natural and artificial sweeteners. PMID: 19083128
    19. Variations in perception of umami taste correlated with variations in the human TAS1R3 gene. PMID: 19587085

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 3 family, TAS1R subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 15661

    OMIM: 605865

    KEGG: hsa:83756

    STRING: 9606.ENSP00000344411

    UniGene: Hs.74375