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Recombinant Human Thymidylate synthase (TYMS)

  • 中文名稱：
    人TYMS重組蛋白
  • 貨號：
    CSB-YP025393HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人TYMS重組蛋白
  • 貨號：
    CSB-EP025393HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人TYMS重組蛋白
  • 貨號：
    CSB-EP025393HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人TYMS重組蛋白
  • 貨號：
    CSB-BP025393HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人TYMS重組蛋白
  • 貨號：
    CSB-MP025393HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    d TMP synthase; EC 2.1.1.45; HsT422; MGC88736; OTTHUMP00000162195; Thymidylate synthase; Thymidylate synthetase; TMS; TS; TSase; Tyms; TYMS protein; Tyms thymidylate synthetase; TYSY_HUMAN
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    2-313
  • 氨基酸序列
    PVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGM QARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSL GFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCA WNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHIT GLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGY NPHPTIKMEMAV
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
  • 基因功能參考文獻(xiàn)：
    1. The nasopharyngeal carcinoma patients with positive LRP and TS expression may be less sensitive to chemotherapy with DDP + 5-FU. PMID: 15952572
    2. high level of expression associated with poor prognosis in p-stage I adenocarcinoma of the lung PMID: 11804689
    3. Length polymorphism of thymidylate synthase regulatory region in Chinese populations and evolution of the novel alleles PMID: 11989786
    4. Mutants of TS that confer resistance to MTX as well as 5-FU by random as well as site-directed mutagenesis. Review. PMID: 12084458
    5. Thymidylate synthase as a translational regulator of cellular gene expression. Review. PMID: 12084459
    6. rTS gene expression likely plays a role in down-regulating TS through a natural RNA-based antisense mechanism. Review. PMID: 12084460
    7. Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Review. PMID: 12084461
    8. Structure-based studies on species-specific inhibition of thymidylate synthase. Review. PMID: 12084462
    9. mutations in TS result in conformational changes that confer 5-fluordeoxyuridine resistance PMID: 12147691
    10. TYMS and MTHFR compete for limiting supplies of folate required for the remethylation of homocysteine. PMID: 12215845
    11. the identified highly conserved protein domains, which occur at the homodimeric interface of thymidylate synthase(TS), represent potential participating sites for binding of TS protein to its mRNA. PMID: 12220503
    12. Enhanced expression mediates resistance of uterine cervical cancer cells to radiation PMID: 12239455
    13. cytomegalovirus infection induces cellular thymidylate synthase gene expression in quiescent human embryonic lung fibroblasts PMID: 12466474
    14. Gene expression is a predictor for the efficacy of fluoropyrimidine-based chemotherapy for metastatic colorectal cancer. PMID: 12576451
    15. Expression predicts the reponse of metastatic colorectal cancer to raltitrexel. PMID: 12576452
    16. Associations between polymorphisms in the thymidylate synthase and serine hydroxymethyltransferase genes and susceptibility to malignant lymphoma. PMID: 12604405
    17. polymorphism and overexpression of Thymidylate synthase mrna is associated with non-small-cell lung cancer PMID: 12640689
    18. Results suggest that the genotyping for thymidylate synthase and methylenetetrahydrofolate reductase polymorphisms may be a useful indicator in determining the appropriate dose of methotrexate in patients with rheumatoid arthritis. PMID: 12684695
    19. Overexpression of thymidylate synthase mediates desensitization for 5-fluorouracil of cervical cancer cells PMID: 12845668
    20. Results describe the kinetic parameters of human recombinant thymidylate synthase (hrTS) with its natural substrate, dUMP, and E-5-(2-bromovinyl)-2(')-deoxyuridine monophosphate (BVdUMP). PMID: 12859954
    21. Findings identify Cys-180 as a critical residue for the in vitro and in vivo translational regulatory effects of human thymidylate synthase. PMID: 12907731
    22. TS and DPD quantitation may be helpful to evaluate prognosis of patients receiving adjuvant 5-FU and that patients with high TS and low DPD may benefit from adjuvant 5-FU chemotherapy in colorectal cancer. PMID: 14519634
    23. High TS expression is a marker of poor prognosis in resected pancreatic cancer. Patients with high intratumoral TS expression benefit from adjuvant therapy. PMID: 14519641
    24. These results indicate that postoperative chemotherapy with 5-FU could be effective for patients with the G-phenotype tumor, since the incidence of intratumoral expression of TS, the target enzyme of 5-FU, is significantly low in G-phenotype tumors PMID: 14576935
    25. There is prognostic significance to TS mRNA expression in breast neoplasms. PMID: 14702180
    26. thymidylate synthase loss of heterozygosity has a role in progression of colorectal cancer PMID: 14760062
    27. Molecular features of thymidylate synthase that control its degradation are examined; the carboxyl-terminal conformational shift is not required for ligand-mediated stabilization; the amino-terminus governs TS stability and its response to ligands. PMID: 14967037
    28. TYMS gene amplification in 23% of 31 5-FU-treated cancers, whereas no amplification was observed in metastases of patients that had not been treated with 5-FU. PMID: 14970324
    29. Over expression of Thymidylate synthase exhibits oncogene-like activity and suggest a link between DNA synthesis and the induction of a neoplastic phenotype PMID: 15093541
    30. 6 bp/1494 polymorphism varies greatly within different ethnic populations and is in linkage disequilibrium with the thymidylate synthase 5' tandem repeat enhancer polymorphism PMID: 15115918
    31. Polymorphism of TYMS partially modifies the risk of esophageal and stomach cancer in patients with smoking habit. PMID: 15244514
    32. Thymidylate synthase inhibition triggers glucose-dependent apoptosis in p53-negative leukemic cells PMID: 15251465
    33. The combination of low expression of TS and induction of p16(INK4a) after chemotherapy can be important indicators of the sensitivity to 5-FU-based chemotherapy in colorectal cancers. PMID: 15260847
    34. Thymidylate synthase polymorphisms are associated with esophageal squamous cell carcinoma and gastric cardiac adenocarcinoma PMID: 15284183
    35. TS in malignant tissue was statistically higher than in normal stromal tissue in both differentiated and undifferentiated types (p < 0.0001). PMID: 15316940
    36. Polymorphisms in the TS gene may contribute to gastric cancer susceptibility. PMID: 15386366
    37. Polymorphisms of reduced folate carrier,aminoimidazole carboxamide ribonucleotide transformylase,and thymidylate synthase genes contribute to the therapeutic response in rheumatoid arthritis patients to methotrexate. PMID: 15457444
    38. The common MTHFR C677T and TS enhancer region polymorphisms were not risk factors for breast cancer in this patient cohort nor were they associated with phenotypic features or with prognosis. PMID: 15510613
    39. Thymidylate synthase polymorphism may have a role in colorectal cancer PMID: 15571262
    40. there is a high level of homology in polymorphic status of thymidylate synthase between normal and malignant colonic tissues PMID: 15571263
    41. thymidylate synthase is inhibited by trifluorothymidine in human tumor cells PMID: 15571283
    42. Thymidylate synthase 5'- and 3'-untranslated region polymorphisms are associated with risk and progression of squamous cell carcinoma of the head and neck PMID: 15585623
    43. Our findings suggest that the TYMS 3'UTR del/del genotype is a significant determinant of elevated RBC folate concentration in a non-smoking population PMID: 15682292
    44. Investigators found a relationship between the TSmRNA expression, evaluated by real-time PCR, and with the TS level determined by immunohistochemical assay in colorectal cancer. PMID: 15736425
    45. High TS1 gene expression is associated with chemotherapy resistance in esophageal cancer PMID: 15788669
    46. Important role for folate deficiency and impaired TS activity in the etiology of esophagel and gastric cancer. PMID: 15817609
    47. Polymorphisms in the 5'- and 3'-untranslated regions are a risk factor for gastric cancer in China. PMID: 15930032
    48. TS polymorphism may be a genetic determinant of plasma homocysteine level in Korean patients with recurrent spontaneous abortion. PMID: 15985285
    49. thymidylate synthase and dihydropyrimidine dehydrogenase are involved in resistance to 5-fluorouracil in human lung cancer cells PMID: 15993511
    50. thymidylate synthase and orotate phosphoribosyl transferase, but not dihydropyrimidine dehydrogenase, are more highly expressed in prostate cancer than in benign prostatic hyperplasia PMID: 15999119

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
  • 蛋白家族：
    Thymidylate synthase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12441

    OMIM: 188350

    KEGG: hsa:7298

    STRING: 9606.ENSP00000315644

    UniGene: Hs.369762