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  4. Recombinant Human Transcription factor AP-2-alpha (TFAP2A)

Recombinant Human Transcription factor AP-2-alpha (TFAP2A)

  • 中文名稱：
    人TFAP2A重組蛋白
  • 貨號(hào)：
    CSB-YP023414HU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    人TFAP2A重組蛋白
  • 貨號(hào)：
    CSB-EP023414HU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    人TFAP2A重組蛋白
  • 貨號(hào)：
    CSB-EP023414HU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人TFAP2A重組蛋白
  • 貨號(hào)：
    CSB-BP023414HU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人TFAP2A重組蛋白
  • 貨號(hào)：
    CSB-MP023414HU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TFAP2A
  • Uniprot No.：
  • 別名：
    Activating enhancer binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP 2 transcription factor; AP 2alpha; AP-2; AP-2 transcription factor; AP2; AP2 Transcription Factor; AP2-alpha; AP2A_HUMAN; AP2TF; BOFS; FLJ51761; TFAP 2; TFAP 2A; TFAP2; TFAP2A; Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha; Transcription factor AP2 alpha
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-437
  • 氨基酸序列
    MLWKLTDNIK YEDCEDRHDG TSNGTARLPQ LGTVGQSPYT SAPPLSHTPN ADFQPPYFPP PYQPIYPQSQ DPYSHVNDPY SLNPLHAQPQ PQHPGWPGQR QSQESGLLHT HRGLPHQLSG LDPRRDYRRH EDLLHGPHAL SSGLGDLSIH SLPHAIEEVP HVEDPGINIP DQTVIKKGPV SLSKSNSNAV SAIPINKDNL FGGVVNPNEV FCSVPGRLSL LSSTSKYKVT VAEVQRRLSP PECLNASLLG GVLRRAKSKN GGRSLREKLD KIGLNLPAGR RKAANVTLLT SLVEGEAVHL ARDFGYVCET EFPAKAVAEF LNRQHSDPNE QVTRKNMLLA TKQICKEFTD LLAQDRSPLG NSRPNPILEP GIQSCLTHFN LISHGFGSPA VCAAVTALQN YLTEALKAMD KMYLSNNPNS HTDNNAKSSD KEEKHRK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
  • 基因功能參考文獻(xiàn)：
    1. The data suggest that IRF6, TFAP2A, and GRHL3, among others, are shared in neural tube and orofacial development. PMID: 27933721
    2. Results show that gout-associated increased NRBP1 expression is regulated through methylation-dependent TFAP2A binding to the B1 region, which might be involved in the pathogenesis of gout. PMID: 28932319
    3. our results indicate that AP-2alpha can reverse the Multidrug resistance (MDR) of gastric cancer cells, which may be realized by inhibiting the Notch signaling pathway. PMID: 28439677
    4. High-confidence AP2alpha-binding peaks were detected in the regulatory regions of many target genes involved in the development of facial tissues including MSX1, IRF6, TBX22, and MAFB. In addition, we uncovered multiple single-nucleotide polymorphisms (SNPs) disrupting a conserved AP2alpha consensus sequence. PMID: 24641171
    5. We identified two SLN genes (PIGR and TFAP2A) that provided high prognostic accuracy in SLN-positive melanoma patients (AUC = 0.864). These two SLN genes, along with clinicopathological features, can differentiate the high- and low-risk groups in node-positive melanoma patients PMID: 27663566
    6. We identified miR-1254 as a negative regulator inhibiting HO-1 translation by directly targeting HO-1 3'UTR via its seed region, and suppressing HO-1 transcription via non-seed region-dependent inhibition of transcriptional factor AP-2 alpha (TFAP2A), a transcriptional activator of HO-1. PMID: 28749936
    7. dimerization-defective mutant of Nef failed to interact with either CD4 or AP-2 in the BiFC assay, indicating that Nef quaternary structure is required for CD4 and AP-2 recruitment as well as CD4 down-regulation PMID: 28031466
    8. Data show that TFAP2A binds many of the same regulatory elements as MITF in melanocytes. PMID: 28249010
    9. the atrial fibrillation (AF)-associated SNP rs2595104 altered PITX2c expression via interaction with TFAP2a; such a pathway could ultimately contribute to AF susceptibility at the PITX2 locus associated with AF PMID: 27866707
    10. AP-2a is an important transcription factor of DEK expression, which is correlated with the methylation level of the DEK core promoter in hepatocellular carcinoma . PMID: 27499261
    11. AP-2alpha expression has a role in human hepatocellular cancer by regulating signaling to affect cell growth and migration PMID: 26780928
    12. role in the expression of Latent membrane protein 1 PMID: 26819314
    13. Hepatitis B virus X protein is able to elevate the expression of SPHK1 in hepatoma cells by upregulating transcription factor AP2 alpha. PMID: 26073327
    14. Results indicate that AP-2alpha activates COX-2 expression to promote NPC growth. PMID: 25669978
    15. The AP-2alpha transcription factor may play an important role in suppressing glioma progression. PMID: 25674231
    16. TFAP2A might play a role in the development of Ovarian Cancer, and may be a therapeutic target in OC. PMID: 25953442
    17. INHA gene expression is upregulated by cAMP via CRE in human trophoblasts, and TFAP2 regulates this expression by interacting with CRE. PMID: 25358080
    18. Human melanomas display higher than normal CpG DNA methylation at the TFAP2A promoter.Aberrant CpG DNA methylation as an epigenetic mark associated with TFAP2A silencing in human melanoma. PMID: 25625848
    19. ETS1 induction of syncytiotrophoblast marker genes likely results in part from transactivation by activator protein-2alpha. PMID: 25651508
    20. Direct sequencing of the coding region of the TFAP2A gene revealed missense mutations in four branchio-oculo-facial syndrome patients; one patient was observed to have a previously described mutation (p.Arg251Gly), while three patients from two families were found to have novel mutations: p.Arg213Ser and p.Val210Asp. PMID: 25590586
    21. AP-2alpha continues to be required after lens vesicle separation to maintain a normal lens epithelial cell phenotype and overall lens integrity and to ensure correct fiber cell differentiation. PMID: 24753151
    22. Cigarette smoke condensate decreased AP-2alpha expression by suppressing its transcription in human lung cancer cell lines, particularly in p53-deficient NCI-H1299 cells. PMID: 25050743
    23. AP2-mediated receptor internalization can be dissociated from AP2-mediated chemotaxis PMID: 24450359
    24. During pregnancy, regulation of hCG beta subunit genes expression correlates with both methylation of their promoters and TFAP2A expression level. PMID: 25063728
    25. TFAP2A regulates nasopharyngeal carcinoma growth and survival through the modulation of the HIF-1alpha-mediated VEGF/PEDF signaling pathway. PMID: 24335623
    26. These data define a mechanism through which AP-2alpha acetylation and increased promoter access induce the expression of the TLR2 gene. PMID: 23680675
    27. Hoxa7, Hoxa9 and Hox cofactor Meis1 were identified as AP-2alpha target genes, which are involved in myeloid leukemogenesis. PMID: 23660297
    28. The present study examined the expression of AP-2alpha, TIMP-2, MMP-2, MMP-9, and E-cadherin in severe preeclamptic placentas and normal placentas. PMID: 23660954
    29. Result suggests that the individual TFAP2A Branchio-Oculo-Facial Syndrome mutations can generate null alleles. These mutations combined with a role for AP-2alpha in epigenetic events, may influence the resultant pathology and the phenotypic variability. PMID: 23578821
    30. TFAP2C repressed CD44 expression in basal-derived breast cancer. PMID: 23764310
    31. Molecular modeling of the HIV-1 Nef C-terminal flexible loop in complex with AP2 suggests that M168L170 occupies a pocket in the AP2 sigma2 subunit. PMID: 23151229
    32. ESDN and AP-2g expression is lower in thick melanomas, it is associated with unfavourable histo-pathological parameters (increased vascularity, vascular invasion and mitoses) and correlates with a shorter DFS like for AP-2a. PMID: 23036739
    33. results indicate that Kctd15 acts in the embryo at least in part by specifically binding to the activation domain of AP-2alpha, thereby blocking the function of this critical factor in the neural crest induction hierarchy. PMID: 23382213
    34. Evidence of association was obtained with both single marker and haplotype analyses, thus providing a support for TFAP2A in non-syndromic cleft lip with or without cleft palate aetiology PMID: 21781438
    35. The different pattern of activator protein-2alpha expression in mild and severe preeclampsia clearly suggests that these conditions may have 2 independent pathogenic mechanisms. PMID: 22575257
    36. A mutation in the TFAP2A gene associated with Branchio-Oculo-Facial syndrome (heterozygous H384Y in exon 7) was found in both the proband and her mother. PMID: 22191992
    37. a robust transcriptional CTNNAL1 up-regulation occurs during acute ozone-induced stress and is mediated at least in part by ozone-induced recruitments of LEF-1 and AP-2alpha to the human CTNNAL1 promoter. PMID: 22359570
    38. fcho1 morphant phenotype is distinct from severe embryonic defects apparent when AP-2 is depleted PMID: 22484487
    39. findings suggest that TFAP2A isoforms may be differentially regulated during breast tumourigenesis and this, coupled with differences in their transcriptional activity, may impact on tumour responses to tamoxifen therapy PMID: 21375726
    40. AP2 regulates the inhibin alpha subunit gene expression during trophoblast differentiation and may be a key regulator of syncytialization PMID: 21659426
    41. Data suggest that AP-2alpha plays an important role in tumor progression and that reduced AP-2alpha expression independently predicts an unfavorable prognosis in gastric adenocarcinoma patients. PMID: 21966377
    42. AP-2alpha regulation of CHRNA7 mRNA expression in multiple tissues during development PMID: 21979958
    43. Bcl-2 gene transcription is regulated by Ap2alpha-Rb complex binding to the Bcl-2 promoter. PMID: 21850486
    44. findings provide a better understanding of the epigenetic mechanisms that are involved in the loss of AP-2alpha protein in prostate cancer cells which lead to decreased cellular zinc uptake-a sine qua non of prostate cancer development PMID: 21940908
    45. Patients with Branchio-Oculo-Facial Syndrome (BOFS) and predominant ocular phenotypes can be diagnosed by upper lip examination. TFAP2A analysis provides diagnostic confirmation and improves genetic counselling. PMID: 21728810
    46. Data demonstrate a cross-talk between IGF-1R and AT-1R in AT-II and IGF-1-induced Cx43 expression in SV SMCs involving Erk 1/2 and downstream activation of the AP-1 transcription factor. PMID: 20731749
    47. A protein-binding microarrays-based study of human healthy and breast tumor tissue extracts allowed the identification of previously unknown AP2alpha target genes. PMID: 21876733
    48. These data suggest a novel cellular function of CK-2 as a transcriptional co-activator of AP-2alpha. PMID: 21777522
    49. Multiple isoforms of AP-2alpha are highly expressed in pancreatic cancer cell lines including a new isoform, AP-2alpha variant 6, which seems to be pancreatic cancer specific and is deprived of transcriptional activity. PMID: 21654541
    50. this study provides further evidence regarding the role of IRF6 variations in non-syndromic cleft lip with or without cleft palate (NSCLP) development and finds no significant association between TFAP2A and NSCLP in this northern Chinese population. PMID: 21683068

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  • 相關(guān)疾?。?/div>
    Branchiooculofacial syndrome (BOFS)
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    AP-2 family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 11742

    OMIM: 107580

    KEGG: hsa:7020

    STRING: 9606.ENSP00000368924

    UniGene: Hs.519880