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  4. Recombinant Human Transient receptor potential cation channel subfamily M member 1 (TRPM1), partial

Recombinant Human Transient receptor potential cation channel subfamily M member 1 (TRPM1), partial

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  • 中文名稱：
    人TRPM1重組蛋白
  • 貨號(hào)：
    CSB-EP773791HU
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    TRPM1
  • Uniprot No.：
  • 別名：
    (Long transient receptor potential channel 1)(LTrpC1)(Melastatin-1)
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長(zhǎng)度：
    Partial
  • 來(lái)源：
    E.coli
  • 分子量：
    61.5 kDa
  • 表達(dá)區(qū)域：
    1127-1603aa
  • 氨基酸序列
    RCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVENMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLRQSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGTSTSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQLTVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEWQCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPDQTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Forms nonselective divalent cation-conducting channels which mediate the influx of Na(2+), Ca(2+), Mg(2+), Mn(2+), Ba(2+), and Ni(2+) into the cytoplasm, leading to membrane depolarization. Impermeable to zinc ions. In addition, forms heteromultimeric ion channels with TRPM3 which are permeable for calcium and zinc ions. Essential for the depolarizing photoresponse of retinal ON bipolar cells. It is part of the GRM6 signaling cascade. May play a role in metastasis suppression. May act as a spontaneously active, calcium-permeable plasma membrane channel.
  • 基因功能參考文獻(xiàn)：
    1. We have confirmed the TRPM1 36,445 bp deletion is a founder mutation in the Ashkenazi-Jewish (AJ) population with a carrier rate of 1 in 50. We have also confirmed the 35,741 bp deletion in the CACNA2D4 gene is a founder mutation in the AJ population with a carrier rate of 1 in 56. PMID: 28726569
    2. These results show that joint tests of main effects and gene-gene interaction reveal associations at some novel loci that were missed when considering main effects alone. PMID: 28813576
    3. Loss of TRPM1 mRNA expression appears to be a crucial event in the progression of melanoma to a more malignant, metastatic phenotype. PMID: 27987328
    4. This study reveals the structural underpinnings of TRPML1's regulation, assembly and pathogenesis. PMID: 28112729
    5. Affected family members harboured the homozygous 1-bp deletion c.2394delC in exon 18 of the TRPM1 gene, whereas their unaffected parents were heterozygous carriers. PMID: 27084085
    6. genotype-phenotype correlations showed that this eye phenotype was secondary to homozygous deletion of TRPM1, the gene responsible for autosomal recessive congenital stationary night blindness. The main differential diagnosis is ceroid lipofuscinosis. PMID: 24668847
    7. these data indicate that purified TRPM1 is mostly dimeric. The three-dimensional structure of TRPM1 dimers is characterized by a small putative transmembrane domain and a larger domain with a hollow cavity. PMID: 25112866
    8. visual deficits in melanoma associated retinopathy are caused by the uptake of TRPM1 autoantibodies into ON-bipolar cells PMID: 23936334
    9. This is the first reported case of a melanoma-associated retinopathy diagnosed utilizing the innovative approach of testing for serum TRPM1 autoantibodies. PMID: 24053997
    10. These data suggest differences in coupling of TRPM1 function to mGluR6 signaling explain different cellular responses to glutamate in the retina and the skin. PMID: 23452348
    11. We found 13 different mutations in the TRPM1 gene in congenital stationary night blindness. PMID: 23714322
    12. The results expand the mutation spectrum of NYX, CACNA1F and GRM6. They also suggest that NYX mutations are a common cause of congenital stationary night blindness (CSNB),No variations were found in TRPM1. PMID: 22735794
    13. present investigation found no evidence for an association between sequence variation at the TRPM1 gene and albumin-to-creatinine ratio in Mexican Americans, although it appears to have modest influence on T2DM risk factors PMID: 21439949
    14. In the human retina TRPM1 is expressed on ON-bipolar cell dendrites that invaginate photoreceptor terminals and is also expressed on the synaptic ribbons of a subclass of rods, suggesting a dual function for TRPM1 in the ON-pathway. PMID: 21896854
    15. A proteomic search for proteins associated with nyctalopin in the retina identifies TRPM1 as the binding partner. PMID: 21832182
    16. This study reveals TRPM1 to be one of the autoantigens targeted by autoantibodies in at least some patients with cancer-associated retinopathy or melanoma-associated retinopathy associated with retinal ON bipolar cell dysfunction. PMID: 21611200
    17. Data demonstrate that TRPM1 proteins are bona fide ion-conducting plasma membrane channels. PMID: 21278253
    18. summarize and discuss our present knowledge of TRPM1: its discovery, expression, regulation, and proposed functions in skin and eye PMID: 21290293
    19. This review covers the significant discoveries related to the physiological function and regulatory mechanism of the TRPM1 channel in retinal ON bipolar cells and the association of human TRPM1 mutations. PMID: 20846719
    20. TRPM1 is mutated in patients with autosomal-recessive congenital stationary night blindness. PMID: 20346272
    21. Human TRPM1 mutations are associated with the complete form of congenital stationary night blindness in Japanese patients. PMID: 20300565
    22. studies identify MITF as a major transcriptional regulator of TRPM1 and suggest that its prognostic value may be linked to MITF-mediated regulation of cellular differentiation PMID: 14744763
    23. These findings suggest that the pattern of TRPM1 mRNA expression may be helpful in the differentiation of Spitz nevi and nodular melanomas. PMID: 19396153
    24. Data suggest that TRPM1 is an ion channel whose function is critical to normal melanocyte pigmentation and is thus a potential target for pigmentation disorders. PMID: 19436059
    25. The regulation of TRPM1 by ultraviolet and its role in melanogenesis in epidermal melanocytes are reported. PMID: 19587221
    26. Recessive mutations of TRPM1 abrogate ON bipolar cell function and cause complete congenital stationary night blindness. PMID: 19878917
    27. [REVIEW] TRPM1 and TRPM2, are localized in intracellular compartments and are involved in melanin synthesis and oxidative stress-induced cell death PMID: 19887679
    28. Mutations in TRPM1 are a common cause of complete congenital stationary night blindness. PMID: 19896109
    29. TRPM1 is mutated in patients with autosomal-recessive complete congenital stationary night blindness. PMID: 19896113

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  • 相關(guān)疾?。?/div>
    Night blindness, congenital stationary, 1C (CSNB1C)
  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell projection, axon.
  • 蛋白家族：
    Transient receptor (TC 1.A.4) family, LTrpC subfamily, TRPM1 sub-subfamily
  • 組織特異性：
    Expressed in the retina where it localizes to the outer plexiform layer. Specifically, it is expressed in retinal bipolar cells (BPCs) of the ON subtype. Highly expressed in benign melanocytic nevi and diffusely expressed in various in situ melanomas, but
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 7146

    OMIM: 603576

    KEGG: hsa:4308

    STRING: 9606.ENSP00000380897

    UniGene: Hs.155942